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A "Turn-on-off-on" fluorescence switch based on quantum dots and gold nanoparticles for discriminative detection of ovotransferrin.
Talanta. 2016 Apr 01; 150:407-14.T

Abstract

A novel strategy for ovotransferrin (OVT) detection by tracing the "on-off-on" fluorescence signals of quantum dots (QDs) and gold nanoparticles (AuNPs) utilizing fluorescence measurements were established in this article. The immune interaction between QDs-OVT and anti-OVT-AuNPs leads to drastic quenching (turning off) of the donor by a fluorescence resonance energy transfer (FRET) process. After the addition of free OVT, anti-OVT-AuNPs peel off from the QDs-OVT surface and bind to free OVT due to competitive immunoreactions, resulting in the restoration of the fluorescence intensity of QDs (turning on). Consequently, this process can be utilized for the selective detection of OVT via optical responses. Under optimal conditions, the fluorescence intensity of the system increased linearly with increasing concentrations of OVT from 0.05 to 3.8 μg/mL. The limit of detection of OVT was 23.55 ng/mL. This method was applied to the analysis of OVT in egg powder samples. Furthermore, the strategy provided in this work could be conveniently followed to establish similar immunosensors for the rapid detection of other proteins with corresponding antibodies.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang Q
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Jin Y
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Fu X
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Ma M
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Cai Z
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China. Electronic address: caizhaoxia@mail.hzau.edu.cn.

MeSH

AnimalsConalbuminFluorescence Resonance Energy TransferGoldHydrogen-Ion ConcentrationKineticsLimit of DetectionMetal NanoparticlesQuantum DotsTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26838424

Citation

Wang, Qi, et al. "A "Turn-on-off-on" Fluorescence Switch Based On Quantum Dots and Gold Nanoparticles for Discriminative Detection of Ovotransferrin." Talanta, vol. 150, 2016, pp. 407-14.
Wang Q, Jin Y, Fu X, et al. A "Turn-on-off-on" fluorescence switch based on quantum dots and gold nanoparticles for discriminative detection of ovotransferrin. Talanta. 2016;150:407-14.
Wang, Q., Jin, Y., Fu, X., Ma, M., & Cai, Z. (2016). A "Turn-on-off-on" fluorescence switch based on quantum dots and gold nanoparticles for discriminative detection of ovotransferrin. Talanta, 150, 407-14. https://doi.org/10.1016/j.talanta.2015.12.068
Wang Q, et al. A "Turn-on-off-on" Fluorescence Switch Based On Quantum Dots and Gold Nanoparticles for Discriminative Detection of Ovotransferrin. Talanta. 2016 Apr 1;150:407-14. PubMed PMID: 26838424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A "Turn-on-off-on" fluorescence switch based on quantum dots and gold nanoparticles for discriminative detection of ovotransferrin. AU - Wang,Qi, AU - Jin,Yongguo, AU - Fu,Xing, AU - Ma,Meihu, AU - Cai,Zhaoxia, Y1 - 2015/12/25/ PY - 2015/10/29/received PY - 2015/12/21/revised PY - 2015/12/24/accepted PY - 2016/2/4/entrez PY - 2016/2/4/pubmed PY - 2016/12/15/medline KW - Anti-ovotransferrin KW - Fluorescence resonance energy transfer KW - Ovotransferrin KW - “Turn-on-off-on” fluorescence switch SP - 407 EP - 14 JF - Talanta JO - Talanta VL - 150 N2 - A novel strategy for ovotransferrin (OVT) detection by tracing the "on-off-on" fluorescence signals of quantum dots (QDs) and gold nanoparticles (AuNPs) utilizing fluorescence measurements were established in this article. The immune interaction between QDs-OVT and anti-OVT-AuNPs leads to drastic quenching (turning off) of the donor by a fluorescence resonance energy transfer (FRET) process. After the addition of free OVT, anti-OVT-AuNPs peel off from the QDs-OVT surface and bind to free OVT due to competitive immunoreactions, resulting in the restoration of the fluorescence intensity of QDs (turning on). Consequently, this process can be utilized for the selective detection of OVT via optical responses. Under optimal conditions, the fluorescence intensity of the system increased linearly with increasing concentrations of OVT from 0.05 to 3.8 μg/mL. The limit of detection of OVT was 23.55 ng/mL. This method was applied to the analysis of OVT in egg powder samples. Furthermore, the strategy provided in this work could be conveniently followed to establish similar immunosensors for the rapid detection of other proteins with corresponding antibodies. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/26838424/A_"Turn_on_off_on"_fluorescence_switch_based_on_quantum_dots_and_gold_nanoparticles_for_discriminative_detection_of_ovotransferrin_ DB - PRIME DP - Unbound Medicine ER -