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Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects of Psidium guajava leaf extracts.
Pharm Biol. 2016 Oct; 54(10):1971-81.PB

Abstract

Context Psidium guajava L. (Myrtaceae) leaves are used in traditional medicines for the treatment of cancer, inflammation and other ailments. Objective The current study explores scientific validation for this traditional medication. Materials and methods We used ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryl hydrazil (DPPH) assays to estimate antioxidant activity of P. guajava leaf extracts (methanol, hexane and chloroform). Antitumour and in vivo cytotoxic activities were determined using potato disc assay (PDA) and brine shrimp lethality assay, respectively. Three human carcinoma cell lines (KBM5, SCC4 and U266) were incubated with different doses (10-100 μg/mL) of extracts and the anticancer activity was estimated by MTT assay. NF-κB suppressing activity was determined using electrophoretic mobility shift assay (EMSA). Chemical composition of the three extracts was identified by GC-MS. Total phenolic and flavonoid contents were measured by colorimetric assays. Results and discussions The order of antioxidant activity of three extracts was methanol > chloroform > hexane. The IC50 values ranged from 22.73 to 51.65 μg/mL for KBM5; 22.82 to 70.25 μg/mL for SCC4 and 20.97 to 89.55 μg/mL for U266 cells. The hexane extract exhibited potent antitumour (IC50 value = 65.02 μg/mL) and cytotoxic (LC50 value = 32.18 μg/mL) activities. This extract also completely inhibited the TNF-α induced NF-κB activation in KBM5 cells. GC-MS results showed that pyrogallol, palmitic acid and vitamin E were the major components of methanol, chloroform and hexane extracts. We observed significant (p < 0.05) difference in total phenolic and flavonoid contents of different solvent extracts. Conclusion The present study demonstrates that P. guajava leaf extracts play a substantial role against cancer and down-modulate inflammatory nuclear factor kB.

Authors+Show Affiliations

a Department of Chemistry , University of Agriculture , Faisalabad , Pakistan ;a Department of Chemistry , University of Agriculture , Faisalabad , Pakistan ; b Central Hi-Tech Laboratory , University of Agriculture , Faisalabad , Pakistan ;a Department of Chemistry , University of Agriculture , Faisalabad , Pakistan ;c Department of Biosciences , COMSATS Institute of Information Technology , Islamabad , Pakistan.a Department of Chemistry , University of Agriculture , Faisalabad , Pakistan ;a Department of Chemistry , University of Agriculture , Faisalabad , Pakistan ;

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26841303

Citation

Ashraf, Aisha, et al. "Chemical Composition, Antioxidant, Antitumor, Anticancer and Cytotoxic Effects of Psidium Guajava Leaf Extracts." Pharmaceutical Biology, vol. 54, no. 10, 2016, pp. 1971-81.
Ashraf A, Sarfraz RA, Rashid MA, et al. Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects of Psidium guajava leaf extracts. Pharm Biol. 2016;54(10):1971-81.
Ashraf, A., Sarfraz, R. A., Rashid, M. A., Mahmood, A., Shahid, M., & Noor, N. (2016). Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects of Psidium guajava leaf extracts. Pharmaceutical Biology, 54(10), 1971-81. https://doi.org/10.3109/13880209.2015.1137604
Ashraf A, et al. Chemical Composition, Antioxidant, Antitumor, Anticancer and Cytotoxic Effects of Psidium Guajava Leaf Extracts. Pharm Biol. 2016;54(10):1971-81. PubMed PMID: 26841303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects of Psidium guajava leaf extracts. AU - Ashraf,Aisha, AU - Sarfraz,Raja Adil, AU - Rashid,Muhammad Abid, AU - Mahmood,Adeel, AU - Shahid,Muhammad, AU - Noor,Nadia, Y1 - 2016/02/03/ PY - 2016/2/4/entrez PY - 2016/2/4/pubmed PY - 2017/2/9/medline KW - A. tunefaciens KW - cell lines KW - flavonoids KW - phenolics SP - 1971 EP - 81 JF - Pharmaceutical biology JO - Pharm Biol VL - 54 IS - 10 N2 - Context Psidium guajava L. (Myrtaceae) leaves are used in traditional medicines for the treatment of cancer, inflammation and other ailments. Objective The current study explores scientific validation for this traditional medication. Materials and methods We used ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryl hydrazil (DPPH) assays to estimate antioxidant activity of P. guajava leaf extracts (methanol, hexane and chloroform). Antitumour and in vivo cytotoxic activities were determined using potato disc assay (PDA) and brine shrimp lethality assay, respectively. Three human carcinoma cell lines (KBM5, SCC4 and U266) were incubated with different doses (10-100 μg/mL) of extracts and the anticancer activity was estimated by MTT assay. NF-κB suppressing activity was determined using electrophoretic mobility shift assay (EMSA). Chemical composition of the three extracts was identified by GC-MS. Total phenolic and flavonoid contents were measured by colorimetric assays. Results and discussions The order of antioxidant activity of three extracts was methanol > chloroform > hexane. The IC50 values ranged from 22.73 to 51.65 μg/mL for KBM5; 22.82 to 70.25 μg/mL for SCC4 and 20.97 to 89.55 μg/mL for U266 cells. The hexane extract exhibited potent antitumour (IC50 value = 65.02 μg/mL) and cytotoxic (LC50 value = 32.18 μg/mL) activities. This extract also completely inhibited the TNF-α induced NF-κB activation in KBM5 cells. GC-MS results showed that pyrogallol, palmitic acid and vitamin E were the major components of methanol, chloroform and hexane extracts. We observed significant (p < 0.05) difference in total phenolic and flavonoid contents of different solvent extracts. Conclusion The present study demonstrates that P. guajava leaf extracts play a substantial role against cancer and down-modulate inflammatory nuclear factor kB. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/26841303/Chemical_composition_antioxidant_antitumor_anticancer_and_cytotoxic_effects_of_Psidium_guajava_leaf_extracts_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2015.1137604 DB - PRIME DP - Unbound Medicine ER -