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Lophirones B and C prevent aflatoxin B1-induced oxidative stress and DNA fragmentation in rat hepatocytes.

Abstract

Context Despite the reported anticarcinogenic activity of lophirones B and C, no scientific information exists for its activity in rat hepatocytes. Objective Effect of lophirones B and C on aflatoxin B1 (AFB1)-induced oxidative stress, and DNA fragmentation in rat hepatocytes was investigated. Materials and methods Wistar rat hepatocytes were incubated with lophirones B and C (1 mg/mL) or sylimarin (1 mg/mL) in the presence or absence of AFB1. For an in vivo study, rats were orally administered with lophirones B and C, and/or AFB1 (20 μg/d) for 9 weeks. Results Lophirones B and C lowered AFB1-mediated increase in nitric oxide, superoxide anion radicals, caspase-3 and fragmented DNA. Lophirones B and C attenuated AFB1-mediated decrease in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and reduced glutathione. Also, lophirones B and C attenuated AFB1-mediated increase in conjugated dienes, lipid hydroperoxides and malondialdehyde in rat hepatocytes. Furthermore, AFB1-mediated alterations in alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin, total bilirubin and globulin in rat serum were significantly annulled in lophirones B and C-treated rats. Conclusion This study revealed that lophirones B and C prevented AFB1-induced oxidative damage in rat hepatocytes.

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  • Authors+Show Affiliations

    ,

    a Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences , Al-Hikmah University , Ilorin , Nigeria ;

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    b Phytomedicine, Toxicology and Reproductive Research Laboratory, Department of Biochemistry , University of Ilorin , Ilorin , Nigeria.

    b Phytomedicine, Toxicology and Reproductive Research Laboratory, Department of Biochemistry , University of Ilorin , Ilorin , Nigeria.

    Source

    Pharmaceutical biology 54:10 2016 Oct pg 1962-70

    MeSH

    Administration, Oral
    Aflatoxin B1
    Animals
    Antioxidants
    Biomarkers
    Chalcones
    Chemical and Drug Induced Liver Injury
    Cytoprotection
    DNA Fragmentation
    Drug Administration Schedule
    Hepatocytes
    Lipid Peroxidation
    Liver Function Tests
    Male
    Oxidative Stress
    Rats
    Rats, Wistar
    Silymarin
    Time Factors

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    26841338