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Echium oil increased the expression of a Δ4 Fads2 fatty acyl desaturase and the deposition of n-3 long-chain polyunsaturated fatty acid in comparison with linseed oil in striped snakehead (Channa striata) muscle.
Fish Physiol Biochem 2016; 42(4):1107-22FP

Abstract

Despite the potential of vegetable oils as aquafeed ingredients, a major drawback associated with their utilization is the inferior level of beneficial n-3 long-chain polyunsaturated fatty acids (LC-PUFA). Echium oil (EO), which is rich in stearidonic acid (SDA, 18:4n-3), could potentially improve the deposition of n-3 LC-PUFA as the biosynthesis of LC-PUFA is enhanced through bypassing the rate-limiting ∆6 desaturation step. We report for the first time an attempt to investigate whether the presence of a desaturase (Fads2) capable of ∆4 desaturation activities and an elongase (Elovl5) will leverage the provision of dietary SDA to produce a higher rate of LC-PUFA bioconversion. Experimental diets were designed containing fish oil (FO), EO or linseed oil (LO) (100FO, 100EO, 100LO), and diets which comprised equal mixtures of the designated oils (50EOFO and 50EOLO) were evaluated in a 12-week feeding trial involving striped snakeheads (Channa striata). There was no significant difference in growth and feed conversion efficiency. The hepatic fatty acid composition and higher expression of fads2 and elovl5 genes in fish fed EO-based diets indicate the utilization of dietary SDA for LC-PUFA biosynthesis. Collectively, this resulted in a higher deposition of muscle eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) compared to LO-based diets. Dietary EO improved the ratio of n-3 LC-PUFA to n-6 LC-PUFA in fish muscle, which is desirable for human populations with excessive consumption of n-6 PUFA. This study validates the contribution of SDA in improving the content of n-3 LC-PUFA and the ratio of EPA to arachidonic acid (ARA, 20:4n-6) in a freshwater carnivorous species.

Authors+Show Affiliations

Centre for Marine and Coastal Studies, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.Centre for Marine and Coastal Studies, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia. alex@usm.my. School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia. alex@usm.my.School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26842427

Citation

Jaya-Ram, Annette, et al. "Echium Oil Increased the Expression of a Δ4 Fads2 Fatty Acyl Desaturase and the Deposition of N-3 Long-chain Polyunsaturated Fatty Acid in Comparison With Linseed Oil in Striped Snakehead (Channa Striata) Muscle." Fish Physiology and Biochemistry, vol. 42, no. 4, 2016, pp. 1107-22.
Jaya-Ram A, Shu-Chien AC, Kuah MK. Echium oil increased the expression of a Δ4 Fads2 fatty acyl desaturase and the deposition of n-3 long-chain polyunsaturated fatty acid in comparison with linseed oil in striped snakehead (Channa striata) muscle. Fish Physiol Biochem. 2016;42(4):1107-22.
Jaya-Ram, A., Shu-Chien, A. C., & Kuah, M. K. (2016). Echium oil increased the expression of a Δ4 Fads2 fatty acyl desaturase and the deposition of n-3 long-chain polyunsaturated fatty acid in comparison with linseed oil in striped snakehead (Channa striata) muscle. Fish Physiology and Biochemistry, 42(4), pp. 1107-22. doi:10.1007/s10695-016-0201-y.
Jaya-Ram A, Shu-Chien AC, Kuah MK. Echium Oil Increased the Expression of a Δ4 Fads2 Fatty Acyl Desaturase and the Deposition of N-3 Long-chain Polyunsaturated Fatty Acid in Comparison With Linseed Oil in Striped Snakehead (Channa Striata) Muscle. Fish Physiol Biochem. 2016;42(4):1107-22. PubMed PMID: 26842427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Echium oil increased the expression of a Δ4 Fads2 fatty acyl desaturase and the deposition of n-3 long-chain polyunsaturated fatty acid in comparison with linseed oil in striped snakehead (Channa striata) muscle. AU - Jaya-Ram,Annette, AU - Shu-Chien,Alexander Chong, AU - Kuah,Meng-Kiat, Y1 - 2016/02/02/ PY - 2015/09/17/received PY - 2016/01/14/accepted PY - 2016/2/5/entrez PY - 2016/2/5/pubmed PY - 2017/3/3/medline KW - Channa striata KW - Echium oil KW - LC-PUFA KW - Nutritional regulation KW - Stearidonic acid KW - ∆4 Desaturase SP - 1107 EP - 22 JF - Fish physiology and biochemistry JO - Fish Physiol. Biochem. VL - 42 IS - 4 N2 - Despite the potential of vegetable oils as aquafeed ingredients, a major drawback associated with their utilization is the inferior level of beneficial n-3 long-chain polyunsaturated fatty acids (LC-PUFA). Echium oil (EO), which is rich in stearidonic acid (SDA, 18:4n-3), could potentially improve the deposition of n-3 LC-PUFA as the biosynthesis of LC-PUFA is enhanced through bypassing the rate-limiting ∆6 desaturation step. We report for the first time an attempt to investigate whether the presence of a desaturase (Fads2) capable of ∆4 desaturation activities and an elongase (Elovl5) will leverage the provision of dietary SDA to produce a higher rate of LC-PUFA bioconversion. Experimental diets were designed containing fish oil (FO), EO or linseed oil (LO) (100FO, 100EO, 100LO), and diets which comprised equal mixtures of the designated oils (50EOFO and 50EOLO) were evaluated in a 12-week feeding trial involving striped snakeheads (Channa striata). There was no significant difference in growth and feed conversion efficiency. The hepatic fatty acid composition and higher expression of fads2 and elovl5 genes in fish fed EO-based diets indicate the utilization of dietary SDA for LC-PUFA biosynthesis. Collectively, this resulted in a higher deposition of muscle eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) compared to LO-based diets. Dietary EO improved the ratio of n-3 LC-PUFA to n-6 LC-PUFA in fish muscle, which is desirable for human populations with excessive consumption of n-6 PUFA. This study validates the contribution of SDA in improving the content of n-3 LC-PUFA and the ratio of EPA to arachidonic acid (ARA, 20:4n-6) in a freshwater carnivorous species. SN - 1573-5168 UR - https://www.unboundmedicine.com/medline/citation/26842427/Echium_oil_increased_the_expression_of_a_Δ4_Fads2_fatty_acyl_desaturase_and_the_deposition_of_n_3_long_chain_polyunsaturated_fatty_acid_in_comparison_with_linseed_oil_in_striped_snakehead__Channa_striata__muscle_ L2 - https://dx.doi.org/10.1007/s10695-016-0201-y DB - PRIME DP - Unbound Medicine ER -