Tags

Type your tag names separated by a space and hit enter

DNA vaccination with a gene encoding Toxoplasma gondii Rhoptry Protein 17 induces partial protective immunity against lethal challenge in mice.
Parasite. 2016; 23:4.P

Abstract

Toxoplasma gondii is an obligate intracellular apicomplexan parasite that affects humans and various vertebrate livestock and causes serious economic losses. To develop an effective vaccine against T. gondii infection, we constructed a DNA vaccine encoding the T. gondii rhoptry protein 17 (TgROP17) and evaluated its immune protective efficacy against acute T. gondii infection in mice. The DNA vaccine (p3×Flag-CMV-14-ROP17) was intramuscularly injected to BALB/c mice and the immune responses of the vaccinated mice were determined. Compared to control mice treated with empty vector or PBS, mice immunized with the ROP17 vaccine showed a relatively high level of specific anti-T. gondii antibodies, and a mixed IgG1/IgG2a response with predominance of IgG2a production. The immunized mice also displayed a specific lymphocyte proliferative response, a Th1-type cellular immune response with production of IFN-γ and interleukin-2, and increased number of CD8(+) T cells. Immunization with the ROP17 DNA significantly prolonged the survival time (15.6 ± 5.4 days, P < 0.05) of mice after challenge infection with the virulent T. gondii RH strain (Type I), compared with the control groups which died within 8 days. Therefore, our data suggest that DNA vaccination with TgROP17 triggers significant humoral and cellular responses and induces effective protection in mice against acute T. gondii infection, indicating that TgROP17 is a promising vaccine candidate against acute toxoplasmosis.

Authors+Show Affiliations

Research Institute of Medical Parasitology, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.Department of General Surgery, Xi'an Red Cross Hospital, Xi'an, Shanxi 710000, PR China.Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92-019, Auckland 1142, New Zealand.Department of Physiology, Key Laboratory of Cellular Physiology Co-Constructed by Province and Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.Research Institute of Medical Parasitology, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26842927

Citation

Wang, Hai-Long, et al. "DNA Vaccination With a Gene Encoding Toxoplasma Gondii Rhoptry Protein 17 Induces Partial Protective Immunity Against Lethal Challenge in Mice." Parasite (Paris, France), vol. 23, 2016, p. 4.
Wang HL, Wang YJ, Pei YJ, et al. DNA vaccination with a gene encoding Toxoplasma gondii Rhoptry Protein 17 induces partial protective immunity against lethal challenge in mice. Parasite. 2016;23:4.
Wang, H. L., Wang, Y. J., Pei, Y. J., Bai, J. Z., Yin, L. T., Guo, R., & Yin, G. R. (2016). DNA vaccination with a gene encoding Toxoplasma gondii Rhoptry Protein 17 induces partial protective immunity against lethal challenge in mice. Parasite (Paris, France), 23, 4. https://doi.org/10.1051/parasite/2016004
Wang HL, et al. DNA Vaccination With a Gene Encoding Toxoplasma Gondii Rhoptry Protein 17 Induces Partial Protective Immunity Against Lethal Challenge in Mice. Parasite. 2016;23:4. PubMed PMID: 26842927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA vaccination with a gene encoding Toxoplasma gondii Rhoptry Protein 17 induces partial protective immunity against lethal challenge in mice. AU - Wang,Hai-Long, AU - Wang,Yu-Jing, AU - Pei,Yan-Jiang, AU - Bai,Ji-Zhong, AU - Yin,Li-Tian, AU - Guo,Rui, AU - Yin,Guo-Rong, Y1 - 2016/02/03/ PY - 2015/11/10/received PY - 2016/01/22/accepted PY - 2016/2/5/entrez PY - 2016/2/5/pubmed PY - 2016/10/25/medline SP - 4 EP - 4 JF - Parasite (Paris, France) JO - Parasite VL - 23 N2 - Toxoplasma gondii is an obligate intracellular apicomplexan parasite that affects humans and various vertebrate livestock and causes serious economic losses. To develop an effective vaccine against T. gondii infection, we constructed a DNA vaccine encoding the T. gondii rhoptry protein 17 (TgROP17) and evaluated its immune protective efficacy against acute T. gondii infection in mice. The DNA vaccine (p3×Flag-CMV-14-ROP17) was intramuscularly injected to BALB/c mice and the immune responses of the vaccinated mice were determined. Compared to control mice treated with empty vector or PBS, mice immunized with the ROP17 vaccine showed a relatively high level of specific anti-T. gondii antibodies, and a mixed IgG1/IgG2a response with predominance of IgG2a production. The immunized mice also displayed a specific lymphocyte proliferative response, a Th1-type cellular immune response with production of IFN-γ and interleukin-2, and increased number of CD8(+) T cells. Immunization with the ROP17 DNA significantly prolonged the survival time (15.6 ± 5.4 days, P < 0.05) of mice after challenge infection with the virulent T. gondii RH strain (Type I), compared with the control groups which died within 8 days. Therefore, our data suggest that DNA vaccination with TgROP17 triggers significant humoral and cellular responses and induces effective protection in mice against acute T. gondii infection, indicating that TgROP17 is a promising vaccine candidate against acute toxoplasmosis. SN - 1776-1042 UR - https://www.unboundmedicine.com/medline/citation/26842927/DNA_vaccination_with_a_gene_encoding_Toxoplasma_gondii_Rhoptry_Protein_17_induces_partial_protective_immunity_against_lethal_challenge_in_mice_ L2 - http://publications.edpsciences.org/10.1051/parasite/2016004 DB - PRIME DP - Unbound Medicine ER -