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The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model.
Oxid Med Cell Longev. 2016; 2016:1594616.OM

Abstract

Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts from Cichorium intybus L. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids) and total antioxidant activity of Red Chicory of Treviso and Variegated Chicory of Castelfranco were evaluated. The bioactivity of polyphenol-rich extracts from chicories was studied in in vitro Caco-2 cell monolayers model. Morphological characteristics changes to test the antioxidant and/or prooxidant effect were verified by histological analysis and observed by Electronic Scansion Microscopy (SEM). On Caco-2 cell model, the polyphenols fractions from chicories have indicated a moderate antioxidant behavior until 17 μM concentration, while 70 μM and 34 μM exert cytotoxic effects for Treviso's and Castelfranco's Chicory, respectively, highlighted by TEER decreasing, increased permeability, and alteration of epithelium. Our findings support the beneficial effects of these products in counteracting the oxidative stress and cellular damage, induced in vitro on Caco-2 cell model, through interaction with the mucopolysaccharide complexes in the glycocalyx, maintaining in vivo a healthy and effective intestinal barrier.

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Authors+Show Affiliations

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Lombardi-Boccia G

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.

MeSH

AntioxidantsCaco-2 CellsChicoryElectric ImpedanceFlavonoidsGlycocalyxGlycosaminoglycansHumansMicroscopy, Electron, ScanningOxidation-ReductionOxidative StressPermeabilityPhytochemicalsPlant ExtractsPolyphenolsReactive Oxygen SpeciesTight Junctions

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26843906

Citation

Azzini, Elena, et al. "The Potential Health Benefits of Polyphenol-Rich Extracts From Cichorium Intybus L. Studied On Caco-2 Cells Model." Oxidative Medicine and Cellular Longevity, vol. 2016, 2016, p. 1594616.

Azzini E, Maiani G, Garaguso I, et al. The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model. Oxid Med Cell Longev. 2016;2016:1594616.

Azzini, E., Maiani, G., Garaguso, I., Polito, A., Foddai, M. S., Venneria, E., Durazzo, A., Intorre, F., Palomba, L., Rauseo, M. L., Lombardi-Boccia, G., & Nobili, F. (2016). The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model. Oxidative Medicine and Cellular Longevity, 2016, 1594616. https://doi.org/10.1155/2016/1594616

Azzini E, et al. The Potential Health Benefits of Polyphenol-Rich Extracts From Cichorium Intybus L. Studied On Caco-2 Cells Model. Oxid Med Cell Longev. 2016;2016:1594616. PubMed PMID: 26843906.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model. AU - Azzini,Elena, AU - Maiani,Giuseppe, AU - Garaguso,Ivana, AU - Polito,Angela, AU - Foddai,Maria S, AU - Venneria,Eugenia, AU - Durazzo,Alessandra, AU - Intorre,Federica, AU - Palomba,Lara, AU - Rauseo,Maria L, AU - Lombardi-Boccia,Ginevra, AU - Nobili,Fabio, Y1 - 2015/12/29/ PY - 2015/09/07/received PY - 2015/10/28/revised PY - 2015/10/28/accepted PY - 2016/2/5/entrez PY - 2016/2/5/pubmed PY - 2016/12/15/medline SP - 1594616 EP - 1594616 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2016 N2 - Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts from Cichorium intybus L. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids) and total antioxidant activity of Red Chicory of Treviso and Variegated Chicory of Castelfranco were evaluated. The bioactivity of polyphenol-rich extracts from chicories was studied in in vitro Caco-2 cell monolayers model. Morphological characteristics changes to test the antioxidant and/or prooxidant effect were verified by histological analysis and observed by Electronic Scansion Microscopy (SEM). On Caco-2 cell model, the polyphenols fractions from chicories have indicated a moderate antioxidant behavior until 17 μM concentration, while 70 μM and 34 μM exert cytotoxic effects for Treviso's and Castelfranco's Chicory, respectively, highlighted by TEER decreasing, increased permeability, and alteration of epithelium. Our findings support the beneficial effects of these products in counteracting the oxidative stress and cellular damage, induced in vitro on Caco-2 cell model, through interaction with the mucopolysaccharide complexes in the glycocalyx, maintaining in vivo a healthy and effective intestinal barrier. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/26843906/The_Potential_Health_Benefits_of_Polyphenol_Rich_Extracts_from_Cichorium_intybus_L__Studied_on_Caco_2_Cells_Model_ DB - PRIME DP - Unbound Medicine ER -