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Aedes aegypti anti-salivary gland antibody concentration and dengue virus exposure history in healthy individuals living in an endemic area in Colombia.
Biomedica. 2015 Oct-Dec; 35(4):572-81.B

Abstract

INTRODUCTION

Mosquito salivary proteins are able to induce an antibody response that reflects the level of human-vector contact. IgG antibodies against dengue virus (DENV-IgG) are indicators of previous exposure. The risk of DENV transmission is not only associated to mosquito or dengue factors, but also to socioeconomic factors that may play an important role in the disease epidemiology.

OBJECTIVE

To determine the effect of the presence of Aedes aegypti mosquitos in different stages in households and the history of dengue exposure on vector-human contact determined by the level of anti-salivary protein antibodies in people living in a Colombian endemic area.

MATERIALS AND METHODS

A pilot study of 58 households and 55 human subjects was conducted in Norte de Santander, Colombia. A questionnaire for socioeconomic factors was administered and houses were examined for the presence of Ae. aegypti specimens in the aquatic stages. The level of DENV-IgG antibodies (DENV-IgG), in addition to IgG and IgM anti- Ae. aegypti salivary gland extract (SGE) antibodies (SGE-IgG, SGE-IgM) were evaluated by ELISA using blood collected in filter paper.

RESULTS

We found a significant higher level of SGE-IgG antibodies in subjects living in houses with Ae. aegypti in aquatic stages. We also found a higher concentration of SGE-IgG antibodies in people exposed to DENV, a positive correlation between IgM-SGE and IgG-DENV and a negative correlation with IgG-SGE.

CONCLUSION

Anti-salivary proteins antibodies are consistent with the presence of Ae. aegypti aquatic stages inside houses and DENV-IgG antibodies concentrations.

Authors+Show Affiliations

Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.Hospital Local Los Patios, Norte de Santander, Colombia.Pathobiological Sciences, Vector-borne Disease Laboratories, School of Veterinary Medicine, Louisiana State University.Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.Pathobiological Sciences, Vector-borne Disease Laboratories, School of Veterinary Medicine, Louisiana State University.Pathobiological Sciences, Vector-borne Disease Laboratories, School of Veterinary Medicine, Louisiana State University.Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA.Department of Pathology, Microbiology and Immunology, University of South Carolina, Columbia, SC, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26844447

Citation

Londoño-Rentería, Berlín, et al. "Aedes Aegypti Anti-salivary Gland Antibody Concentration and Dengue Virus Exposure History in Healthy Individuals Living in an Endemic Area in Colombia." Biomedica : Revista Del Instituto Nacional De Salud, vol. 35, no. 4, 2015, pp. 572-81.
Londoño-Rentería B, Cárdenas JC, Giovanni JE, et al. Aedes aegypti anti-salivary gland antibody concentration and dengue virus exposure history in healthy individuals living in an endemic area in Colombia. Biomedica. 2015;35(4):572-81.
Londoño-Rentería, B., Cárdenas, J. C., Giovanni, J. E., Cárdenas, L., Villamizar, P., Rolón, J., Chisenhall, D. M., Christofferson, R. C., Carvajal, D. J., Pérez, O. G., Wesson, D. M., & Mores, C. N. (2015). Aedes aegypti anti-salivary gland antibody concentration and dengue virus exposure history in healthy individuals living in an endemic area in Colombia. Biomedica : Revista Del Instituto Nacional De Salud, 35(4), 572-81. https://doi.org/10.7705/biomedica.v35i4.2530
Londoño-Rentería B, et al. Aedes Aegypti Anti-salivary Gland Antibody Concentration and Dengue Virus Exposure History in Healthy Individuals Living in an Endemic Area in Colombia. Biomedica. 2015 Oct-Dec;35(4):572-81. PubMed PMID: 26844447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aedes aegypti anti-salivary gland antibody concentration and dengue virus exposure history in healthy individuals living in an endemic area in Colombia. AU - Londoño-Rentería,Berlín, AU - Cárdenas,Jenny C, AU - Giovanni,Jennifer E, AU - Cárdenas,Lucio, AU - Villamizar,Paloma, AU - Rolón,Jennifer, AU - Chisenhall,Daniel M, AU - Christofferson,Rebecca C, AU - Carvajal,Daisy J, AU - Pérez,Omar G, AU - Wesson,Dawn M, AU - Mores,Christopher N, PY - 2014/09/22/received PY - 2015/07/06/accepted PY - 2016/2/5/entrez PY - 2016/2/6/pubmed PY - 2016/12/15/medline SP - 572 EP - 81 JF - Biomedica : revista del Instituto Nacional de Salud JO - Biomedica VL - 35 IS - 4 N2 - INTRODUCTION: Mosquito salivary proteins are able to induce an antibody response that reflects the level of human-vector contact. IgG antibodies against dengue virus (DENV-IgG) are indicators of previous exposure. The risk of DENV transmission is not only associated to mosquito or dengue factors, but also to socioeconomic factors that may play an important role in the disease epidemiology. OBJECTIVE: To determine the effect of the presence of Aedes aegypti mosquitos in different stages in households and the history of dengue exposure on vector-human contact determined by the level of anti-salivary protein antibodies in people living in a Colombian endemic area. MATERIALS AND METHODS: A pilot study of 58 households and 55 human subjects was conducted in Norte de Santander, Colombia. A questionnaire for socioeconomic factors was administered and houses were examined for the presence of Ae. aegypti specimens in the aquatic stages. The level of DENV-IgG antibodies (DENV-IgG), in addition to IgG and IgM anti- Ae. aegypti salivary gland extract (SGE) antibodies (SGE-IgG, SGE-IgM) were evaluated by ELISA using blood collected in filter paper. RESULTS: We found a significant higher level of SGE-IgG antibodies in subjects living in houses with Ae. aegypti in aquatic stages. We also found a higher concentration of SGE-IgG antibodies in people exposed to DENV, a positive correlation between IgM-SGE and IgG-DENV and a negative correlation with IgG-SGE. CONCLUSION: Anti-salivary proteins antibodies are consistent with the presence of Ae. aegypti aquatic stages inside houses and DENV-IgG antibodies concentrations. SN - 2590-7379 UR - https://www.unboundmedicine.com/medline/citation/26844447/Aedes_aegypti_anti_salivary_gland_antibody_concentration_and_dengue_virus_exposure_history_in_healthy_individuals_living_in_an_endemic_area_in_Colombia_ L2 - https://www.revistabiomedica.org/index.php/biomedica/article/view/2530 DB - PRIME DP - Unbound Medicine ER -