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CTRP3 promotes energy production by inducing mitochondrial ROS and up-expression of PGC-1α in vascular smooth muscle cells.
Exp Cell Res. 2016 Feb 15; 341(2):177-86.EC

Abstract

C1q/tumor necrosis factor-related protein-3 (CTRP3) is an adipokine with modulation effects on metabolism and inflammation. Adenosine triphosphate (ATP) exerts multiple biological effects in vascular smooth muscle cells (VSMCs) and energy imbalance is involved in vascular diseases. This study aimed to explore the effect of CTRP3 on energy production and its underlying mechanism in VSMCs. Our results indicated that exogenous CTRP3 increased ATP synthesis and the protein expression of oxidative phosphorylation (OXPHOS)-related molecules, including peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α, sirtuin-3 (SIRT3), complex I, II, III, and V in cultured VSMCs. Depletion of endogenous CTRP3 by small interfering RNA (siRNA) reduced ATP synthesis and the expression of those molecules. PGC-1α knockdown abrogated CTRP3-induced ATP production and OXPHOS-related protein expression. Furthermore, CTRP3 increased mitochondrial reactive oxygen species (ROS) production and mitochondrial membrane potential level. Pretreatment with N-acetyl-L-cysteine, a reactive oxygen species scavenger, and cyanidem-chlorophenylhydrazone, an uncoupler of OXPHOS, suppressed CTRP3-induced ROS production, PGC-1α expression and ATP synthesis. In conclusion, CTRP3 modulates mitochondrial energy production through targets of ROS and PGC-1α in VSMCs.

Authors+Show Affiliations

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, PR China; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, PR China; Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, PR China.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, PR China; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, PR China; Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, PR China.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, PR China; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, PR China; Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, PR China.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, PR China; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, PR China; Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, PR China.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, PR China; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, PR China; Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, PR China.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, PR China; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, PR China; Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, PR China. Electronic address: lilyby@163.com.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, PR China; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, PR China; Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, PR China. Electronic address: pathophy@bjmu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26844631

Citation

Feng, Han, et al. "CTRP3 Promotes Energy Production By Inducing Mitochondrial ROS and Up-expression of PGC-1α in Vascular Smooth Muscle Cells." Experimental Cell Research, vol. 341, no. 2, 2016, pp. 177-86.
Feng H, Wang JY, Zheng M, et al. CTRP3 promotes energy production by inducing mitochondrial ROS and up-expression of PGC-1α in vascular smooth muscle cells. Exp Cell Res. 2016;341(2):177-86.
Feng, H., Wang, J. Y., Zheng, M., Zhang, C. L., An, Y. M., Li, L., & Wu, L. L. (2016). CTRP3 promotes energy production by inducing mitochondrial ROS and up-expression of PGC-1α in vascular smooth muscle cells. Experimental Cell Research, 341(2), 177-86. https://doi.org/10.1016/j.yexcr.2016.02.001
Feng H, et al. CTRP3 Promotes Energy Production By Inducing Mitochondrial ROS and Up-expression of PGC-1α in Vascular Smooth Muscle Cells. Exp Cell Res. 2016 Feb 15;341(2):177-86. PubMed PMID: 26844631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CTRP3 promotes energy production by inducing mitochondrial ROS and up-expression of PGC-1α in vascular smooth muscle cells. AU - Feng,Han, AU - Wang,Jin-Yu, AU - Zheng,Ming, AU - Zhang,Cheng-Lin, AU - An,Yuan-Ming, AU - Li,Li, AU - Wu,Li-Ling, Y1 - 2016/02/02/ PY - 2015/11/12/received PY - 2016/01/29/revised PY - 2016/02/01/accepted PY - 2016/2/5/entrez PY - 2016/2/5/pubmed PY - 2016/8/12/medline KW - CTRP3 KW - Energy metabolism KW - Mitochondrial reactive oxygen species KW - Oxidative phosphorylation KW - Peroxisome proliferator-activated receptor γ co-activator 1-α KW - Vascular smooth muscle cell SP - 177 EP - 86 JF - Experimental cell research JO - Exp. Cell Res. VL - 341 IS - 2 N2 - C1q/tumor necrosis factor-related protein-3 (CTRP3) is an adipokine with modulation effects on metabolism and inflammation. Adenosine triphosphate (ATP) exerts multiple biological effects in vascular smooth muscle cells (VSMCs) and energy imbalance is involved in vascular diseases. This study aimed to explore the effect of CTRP3 on energy production and its underlying mechanism in VSMCs. Our results indicated that exogenous CTRP3 increased ATP synthesis and the protein expression of oxidative phosphorylation (OXPHOS)-related molecules, including peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α, sirtuin-3 (SIRT3), complex I, II, III, and V in cultured VSMCs. Depletion of endogenous CTRP3 by small interfering RNA (siRNA) reduced ATP synthesis and the expression of those molecules. PGC-1α knockdown abrogated CTRP3-induced ATP production and OXPHOS-related protein expression. Furthermore, CTRP3 increased mitochondrial reactive oxygen species (ROS) production and mitochondrial membrane potential level. Pretreatment with N-acetyl-L-cysteine, a reactive oxygen species scavenger, and cyanidem-chlorophenylhydrazone, an uncoupler of OXPHOS, suppressed CTRP3-induced ROS production, PGC-1α expression and ATP synthesis. In conclusion, CTRP3 modulates mitochondrial energy production through targets of ROS and PGC-1α in VSMCs. SN - 1090-2422 UR - https://www.unboundmedicine.com/medline/citation/26844631/CTRP3_promotes_energy_production_by_inducing_mitochondrial_ROS_and_up_expression_of_PGC_1α_in_vascular_smooth_muscle_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(16)30023-4 DB - PRIME DP - Unbound Medicine ER -