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RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking.
Appl Microbiol Biotechnol. 2016 Jun; 100(11):5017-27.AM

Abstract

Glycerol is a key yeast metabolite in winemaking because it contributes to improve the organoleptic properties of wine. It is also a cellular protective molecule that enhances the tolerance of yeasts to osmotic stress and promotes longevity. Thus, its production increases by genetic manipulation, which is of biotechnological and basic interest. Glycerol is produced by diverting glycolytic glyceraldehyde-3-phosphate through the action of glycerol-3-phosphate dehydrogenase (coded by genes GPD1 and GPD2). Here, we demonstrate that RNA-binding protein Pub1p regulates glycerol production by controlling Gpd1p activity. Its deletion does not alter GPD1 mRNA levels, but protein levels and enzymatic activity increase, which explains the higher intracellular glycerol concentration and greater tolerance to osmotic stress of the pub1∆ mutant. PUB1 deletion also enhances the activity of nicotinamidase, a longevity-promoting enzyme. Both enzymatic activities are partially located in peroxisomes, and we detected peroxisome formation during wine fermentation. The role of Pub1p in life span control depends on nutrient conditions and is related with the TOR pathway, and a major connection between RNA metabolism and the nutrient signaling response is established.

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Authors+Show Affiliations

Orozco H
Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain. helena.orozco@uv.es. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain. helena.orozco@uv.es.
Sepúlveda A
Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain.
Picazo C
Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain.
Matallana E
Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain.
Aranda A
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain.

MeSH

FermentationFood HandlingFruit and Vegetable JuicesGene Expression Regulation, FungalGlycerolGlycerol-3-Phosphate Dehydrogenase (NAD+)Glycerolphosphate DehydrogenaseMembrane ProteinsMembrane Transport ProteinsOsmotic PressurePhosphatidylinositol 3-KinasesPoly(A)-Binding ProteinsProtein Serine-Threonine KinasesRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsStress, PhysiologicalVitisWine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26846624

Citation

Orozco, Helena, et al. "RNA Binding Protein Pub1p Regulates Glycerol Production and Stress Tolerance By Controlling Gpd1p Activity During Winemaking." Applied Microbiology and Biotechnology, vol. 100, no. 11, 2016, pp. 5017-27.
Orozco H, Sepúlveda A, Picazo C, et al. RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking. Appl Microbiol Biotechnol. 2016;100(11):5017-27.
Orozco, H., Sepúlveda, A., Picazo, C., Matallana, E., & Aranda, A. (2016). RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking. Applied Microbiology and Biotechnology, 100(11), 5017-27. https://doi.org/10.1007/s00253-016-7340-z
Orozco H, et al. RNA Binding Protein Pub1p Regulates Glycerol Production and Stress Tolerance By Controlling Gpd1p Activity During Winemaking. Appl Microbiol Biotechnol. 2016;100(11):5017-27. PubMed PMID: 26846624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking. AU - Orozco,Helena, AU - Sepúlveda,Ana, AU - Picazo,Cecilia, AU - Matallana,Emilia, AU - Aranda,Agustín, Y1 - 2016/02/04/ PY - 2015/09/17/received PY - 2016/01/17/accepted PY - 2016/01/14/revised PY - 2016/2/6/entrez PY - 2016/2/6/pubmed PY - 2017/2/9/medline KW - Glycerol KW - Gpd1p KW - Peroxisomes KW - Pub1p KW - Saccharomyces cerevisiae KW - TOR1/Sch9 nutrient signaling pathway KW - Winemaking SP - 5017 EP - 27 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 100 IS - 11 N2 - Glycerol is a key yeast metabolite in winemaking because it contributes to improve the organoleptic properties of wine. It is also a cellular protective molecule that enhances the tolerance of yeasts to osmotic stress and promotes longevity. Thus, its production increases by genetic manipulation, which is of biotechnological and basic interest. Glycerol is produced by diverting glycolytic glyceraldehyde-3-phosphate through the action of glycerol-3-phosphate dehydrogenase (coded by genes GPD1 and GPD2). Here, we demonstrate that RNA-binding protein Pub1p regulates glycerol production by controlling Gpd1p activity. Its deletion does not alter GPD1 mRNA levels, but protein levels and enzymatic activity increase, which explains the higher intracellular glycerol concentration and greater tolerance to osmotic stress of the pub1∆ mutant. PUB1 deletion also enhances the activity of nicotinamidase, a longevity-promoting enzyme. Both enzymatic activities are partially located in peroxisomes, and we detected peroxisome formation during wine fermentation. The role of Pub1p in life span control depends on nutrient conditions and is related with the TOR pathway, and a major connection between RNA metabolism and the nutrient signaling response is established. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/26846624/RNA_binding_protein_Pub1p_regulates_glycerol_production_and_stress_tolerance_by_controlling_Gpd1p_activity_during_winemaking_ DB - PRIME DP - Unbound Medicine ER -