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[Integration of phage Mu into the RP4::D3112A-plasmid in Escherichia coli cells].
Genetika. 1989 Aug; 25(8):1384-90.G

Abstract

Hybrid plasmids obtained as a result of Mu phage insertions into the RP4::D3112 plasmid in Escherichia coli cells were studied. Stable maintenance of RP4::D3112 plasmid in E. coli cells was provided by using the D3112 phage genome with a point polar mutation in the A gene which prevented early genes' expression. The presence of D3112A- in the RP4 plasmid has been shown to have no effect on efficiency of phage Mu transposition into this plasmid. Moreover, RP4 and D3112 genomes were equivalent targets for Mu integration. The integration of transposable phage into genome of nonrelated phage can be used as one of the approaches to construct recombinant phage genomes in vivo in the absence of DNA homology.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

2684749

Citation

Kaplan, A M., et al. "[Integration of Phage Mu Into the RP4::D3112A-plasmid in Escherichia Coli Cells]." Genetika, vol. 25, no. 8, 1989, pp. 1384-90.
Kaplan AM, Akhverdian VZ, Krylov VN. [Integration of phage Mu into the RP4::D3112A-plasmid in Escherichia coli cells]. Genetika. 1989;25(8):1384-90.
Kaplan, A. M., Akhverdian, V. Z., & Krylov, V. N. (1989). [Integration of phage Mu into the RP4::D3112A-plasmid in Escherichia coli cells]. Genetika, 25(8), 1384-90.
Kaplan AM, Akhverdian VZ, Krylov VN. [Integration of Phage Mu Into the RP4::D3112A-plasmid in Escherichia Coli Cells]. Genetika. 1989;25(8):1384-90. PubMed PMID: 2684749.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Integration of phage Mu into the RP4::D3112A-plasmid in Escherichia coli cells]. AU - Kaplan,A M, AU - Akhverdian,V Z, AU - Krylov,V N, PY - 1989/8/1/pubmed PY - 1989/8/1/medline PY - 1989/8/1/entrez SP - 1384 EP - 90 JF - Genetika JO - Genetika VL - 25 IS - 8 N2 - Hybrid plasmids obtained as a result of Mu phage insertions into the RP4::D3112 plasmid in Escherichia coli cells were studied. Stable maintenance of RP4::D3112 plasmid in E. coli cells was provided by using the D3112 phage genome with a point polar mutation in the A gene which prevented early genes' expression. The presence of D3112A- in the RP4 plasmid has been shown to have no effect on efficiency of phage Mu transposition into this plasmid. Moreover, RP4 and D3112 genomes were equivalent targets for Mu integration. The integration of transposable phage into genome of nonrelated phage can be used as one of the approaches to construct recombinant phage genomes in vivo in the absence of DNA homology. SN - 0016-6758 UR - https://www.unboundmedicine.com/medline/citation/2684749/[Integration_of_phage_Mu_into_the_RP4::D3112A_plasmid_in_Escherichia_coli_cells]_ DB - PRIME DP - Unbound Medicine ER -