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Detection and characterization of diverse alpha- and betacoronaviruses from bats in China.
Virol Sin. 2016 Feb; 31(1):69-77.VS

Abstract

Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses (CoVs) were detected in 50 of 951 (positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase (RdRP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus. Among the 50 positive specimens, thirteen gave rise to CoV full-length RdRP gene amplification for further sequence comparison, of which three divergent sequences (two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses (JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus (JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed.

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Authors+Show Affiliations

Xu L
Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China.
Zhang F
Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China.
Yang W
Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
Jiang T
School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China.
Lu G
School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China.
He B
Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China.
Li X
Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China.
Hu T
Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China.
Chen G
Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China.
Feng Y
Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
Zhang Y
Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
Fan Q
Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China.
Feng J
School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China.
Zhang H
Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
Tu C
Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China. changchun_tu@hotmail.com. Jiangsu Co-innovation Center for Prevention and Control of Important, Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. changchun_tu@hotmail.com.

MeSH

AnimalsBase SequenceChinaChiropteraCoronavirusCoronavirus InfectionsEvolution, MolecularGenetic VariationGenome, ViralPhylogenyRNA-Dependent RNA PolymeraseReal-Time Polymerase Chain ReactionSequence Analysis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26847648

Citation

Xu, Lin, et al. "Detection and Characterization of Diverse Alpha- and Betacoronaviruses From Bats in China." Virologica Sinica, vol. 31, no. 1, 2016, pp. 69-77.
Xu L, Zhang F, Yang W, et al. Detection and characterization of diverse alpha- and betacoronaviruses from bats in China. Virol Sin. 2016;31(1):69-77.
Xu, L., Zhang, F., Yang, W., Jiang, T., Lu, G., He, B., Li, X., Hu, T., Chen, G., Feng, Y., Zhang, Y., Fan, Q., Feng, J., Zhang, H., & Tu, C. (2016). Detection and characterization of diverse alpha- and betacoronaviruses from bats in China. Virologica Sinica, 31(1), 69-77. https://doi.org/10.1007/s12250-016-3727-3
Xu L, et al. Detection and Characterization of Diverse Alpha- and Betacoronaviruses From Bats in China. Virol Sin. 2016;31(1):69-77. PubMed PMID: 26847648.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and characterization of diverse alpha- and betacoronaviruses from bats in China. AU - Xu,Lin, AU - Zhang,Fuqiang, AU - Yang,Weihong, AU - Jiang,Tinglei, AU - Lu,Guanjun, AU - He,Biao, AU - Li,Xingyu, AU - Hu,Tingsong, AU - Chen,Gang, AU - Feng,Yun, AU - Zhang,Yuzhen, AU - Fan,Quanshui, AU - Feng,Jiang, AU - Zhang,Hailin, AU - Tu,Changchun, Y1 - 2016/02/02/ PY - 2016/01/15/received PY - 2016/01/21/accepted PY - 2016/2/6/entrez PY - 2016/2/6/pubmed PY - 2016/12/15/medline KW - Alphacoronavirus KW - Betacoronavirus KW - bats KW - diversity SP - 69 EP - 77 JF - Virologica Sinica JO - Virol Sin VL - 31 IS - 1 N2 - Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses (CoVs) were detected in 50 of 951 (positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase (RdRP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus. Among the 50 positive specimens, thirteen gave rise to CoV full-length RdRP gene amplification for further sequence comparison, of which three divergent sequences (two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses (JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus (JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed. SN - 1995-820X UR - https://www.unboundmedicine.com/medline/citation/26847648/Detection_and_characterization_of_diverse_alpha__and_betacoronaviruses_from_bats_in_China_ DB - PRIME DP - Unbound Medicine ER -