Tags

Type your tag names separated by a space and hit enter

Expression profile of hydrogen sulfide and its synthases correlates with tumor stage and grade in urothelial cell carcinoma of bladder.
Urol Oncol. 2016 Apr; 34(4):166.e15-20.UO

Abstract

BACKGROUND

Hydrogen sulfide (H2S) is a newly discovered gas transmitter. It is synthesized by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MPST). Endogenous hydrogen sulfide has never been studied in bladder cancer.

PURPOSE

We evaluated H2S production and its synthases expression levels in transitional cell carcinoma (urothelial cell carcinoma of bladder [UCB]) of human bladder tissue and cell lines.

MATERIALS AND METHODS

Immunostaining was performed in urothelial cell lines and bladder specimens from 94 patients with UCB of different stages/grades. The expression levels/activities of CBS, CSE, and MPST of specimens and cell lines were analyzed by image semiquantity assay, western blot, and a sulfur-sensitive electrode. We tried to find the correlation between hydrogen sulfide and its synthases with tumor stage in UCB. All experiments were repeated at least 3 times.

RESULTS

Immunoreactivity for CBS, CSE, and MPST was detected in malignant uroepithelium and muscular layer of all tissues examined and cultured cells. The expression levels of CBS, CSE, and MPST were associated with UCB stage/grade. Muscle-invasive bladder cancer samples showed the highest production of H2S (52.6±2.91 nmol/[mg·min]) among all tested samples and EJ cells (transitional cell carcinoma, grade IIIshowed the highest production of H2S among all tested cell lines (53.3±7.02nmol/[mg·min]).

CONCLUSIONS

Protein levels and catalytic activities of CBS, CSE, and MPST increased with the increase of malignant degrees in human bladder tissues and human UCB cell lines. Our findings may promote the application of these novel enzymes to UCB diagnosis or treatment.

Authors+Show Affiliations

Department of Urology, Tianjin First Central Hospital, Tianjin, P.R. China.Department of orthopedics, Xiangyang Central Hospital, Xiangyang, P.R. China.Hedong Center for Disease Control and Prevention, Tianjin, P.R. China.Department of Urology, Tianjin First Central Hospital, Tianjin, P.R. China.Department of Urology, First Hospital of Shanxi Medical University, Taiyuan, P.R. China.Department of Urology, The People׳s Hospital of Nanpi Country, Nanpi, P.R. China.Department of Urology, Tianjin First Central Hospital, Tianjin, P.R. China.Department of Urology, Tianjin First Central Hospital, Tianjin, P.R. China.Department of Urology, Tianjin First Central Hospital, Tianjin, P.R. China.Department of Urology, Tianjin First Central Hospital, Tianjin, P.R. China.Department of Urology, Peking University First Hospital, The Institute of Urology, Peking University, Beijing, P.R. China.Department of Urology, Tianjin First Central Hospital, Tianjin, P.R. China. Electronic address: ghduro@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26847849

Citation

Gai, Jun-Wei, et al. "Expression Profile of Hydrogen Sulfide and Its Synthases Correlates With Tumor Stage and Grade in Urothelial Cell Carcinoma of Bladder." Urologic Oncology, vol. 34, no. 4, 2016, pp. 166.e15-20.
Gai JW, Qin W, Liu M, et al. Expression profile of hydrogen sulfide and its synthases correlates with tumor stage and grade in urothelial cell carcinoma of bladder. Urol Oncol. 2016;34(4):166.e15-20.
Gai, J. W., Qin, W., Liu, M., Wang, H. F., Zhang, M., Li, M., Zhou, W. H., Ma, Q. T., Liu, G. M., Song, W. H., Jin, J., & Ma, H. S. (2016). Expression profile of hydrogen sulfide and its synthases correlates with tumor stage and grade in urothelial cell carcinoma of bladder. Urologic Oncology, 34(4), e15-20. https://doi.org/10.1016/j.urolonc.2015.06.020
Gai JW, et al. Expression Profile of Hydrogen Sulfide and Its Synthases Correlates With Tumor Stage and Grade in Urothelial Cell Carcinoma of Bladder. Urol Oncol. 2016;34(4):166.e15-20. PubMed PMID: 26847849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profile of hydrogen sulfide and its synthases correlates with tumor stage and grade in urothelial cell carcinoma of bladder. AU - Gai,Jun-Wei, AU - Qin,Wei, AU - Liu,Miao, AU - Wang,Hai-Feng, AU - Zhang,Min, AU - Li,Meng, AU - Zhou,Wen-Hui, AU - Ma,Qin-Tong, AU - Liu,Guang-Ming, AU - Song,Wen-Hui, AU - Jin,Jie, AU - Ma,Hong-Shun, Y1 - 2016/02/01/ PY - 2015/01/17/received PY - 2015/06/27/revised PY - 2015/06/29/accepted PY - 2016/2/6/entrez PY - 2016/2/6/pubmed PY - 2017/1/19/medline KW - 3-Mercaptopyruvate sulfurtransferase KW - Cystathionine β-synthase KW - Cystathionine γ-lyase KW - Hydrogen sulfide KW - Urothelial cell carcinoma of bladder SP - 166.e15 EP - 20 JF - Urologic oncology JO - Urol Oncol VL - 34 IS - 4 N2 - BACKGROUND: Hydrogen sulfide (H2S) is a newly discovered gas transmitter. It is synthesized by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MPST). Endogenous hydrogen sulfide has never been studied in bladder cancer. PURPOSE: We evaluated H2S production and its synthases expression levels in transitional cell carcinoma (urothelial cell carcinoma of bladder [UCB]) of human bladder tissue and cell lines. MATERIALS AND METHODS: Immunostaining was performed in urothelial cell lines and bladder specimens from 94 patients with UCB of different stages/grades. The expression levels/activities of CBS, CSE, and MPST of specimens and cell lines were analyzed by image semiquantity assay, western blot, and a sulfur-sensitive electrode. We tried to find the correlation between hydrogen sulfide and its synthases with tumor stage in UCB. All experiments were repeated at least 3 times. RESULTS: Immunoreactivity for CBS, CSE, and MPST was detected in malignant uroepithelium and muscular layer of all tissues examined and cultured cells. The expression levels of CBS, CSE, and MPST were associated with UCB stage/grade. Muscle-invasive bladder cancer samples showed the highest production of H2S (52.6±2.91 nmol/[mg·min]) among all tested samples and EJ cells (transitional cell carcinoma, grade IIIshowed the highest production of H2S among all tested cell lines (53.3±7.02nmol/[mg·min]). CONCLUSIONS: Protein levels and catalytic activities of CBS, CSE, and MPST increased with the increase of malignant degrees in human bladder tissues and human UCB cell lines. Our findings may promote the application of these novel enzymes to UCB diagnosis or treatment. SN - 1873-2496 UR - https://www.unboundmedicine.com/medline/citation/26847849/Expression_profile_of_hydrogen_sulfide_and_its_synthases_correlates_with_tumor_stage_and_grade_in_urothelial_cell_carcinoma_of_bladder_ DB - PRIME DP - Unbound Medicine ER -