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Effect of dietary fat composition on rat colon plasma membranes and fecal lipids.
J Nutr. 1989 Oct; 119(10):1376-82.JN

Abstract

The present study was designed to examine the effect of dietary fat composition on the structure of colon mucosal plasma membranes and fecal lipids. Rats were fed a purified diet containing 14% of either highly saturated fat (beef fat or butterfat) or highly polyunsaturated oil (safflower) in addition to 2% corn oil for 4 wk. Colon mucosal membranes were prepared and examined for lipid composition and protein pattern. Saturated fatty acid feeding resulted in the loss of some protein bands from plasma membranes compared to feeding polyunsaturated fatty acids. Within the saturated fatty acid--rich fats, feeding beef fat caused a greater loss than did feeding butterfat. Dietary fat composition had no effect on membrane content of phospholipid and cholesterol. Saturated fatty acid feeding resulted in an increase in the percentage of 18:1 in plasma membrane lipids compared to feeding safflower oil. The observed changes in the structure of colon mucosal membrane of animals fed the saturated fats were associated with an increase in fecal free fatty acids. There was a 4-fold and 2-fold increase in fecal free fatty acids with feeding the beef fat and butterfat diets, respectively, compared to the safflower oil diet. Alterations in fecal bile acid and free fatty acid composition were also noticed with feeding saturated fatty acids. The results obtained suggest that feeding saturated fatty acids as the main source of fat in the diet could influence the structure of colon mucosa, and this could be mediated through fecal free fatty acids.

Authors+Show Affiliations

Nutrition Program, State University of New York, Buffalo 14214.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

2685200

Citation

Awad, A B., et al. "Effect of Dietary Fat Composition On Rat Colon Plasma Membranes and Fecal Lipids." The Journal of Nutrition, vol. 119, no. 10, 1989, pp. 1376-82.
Awad AB, Chattopadhyay JP, Danahy ME. Effect of dietary fat composition on rat colon plasma membranes and fecal lipids. J Nutr. 1989;119(10):1376-82.
Awad, A. B., Chattopadhyay, J. P., & Danahy, M. E. (1989). Effect of dietary fat composition on rat colon plasma membranes and fecal lipids. The Journal of Nutrition, 119(10), 1376-82.
Awad AB, Chattopadhyay JP, Danahy ME. Effect of Dietary Fat Composition On Rat Colon Plasma Membranes and Fecal Lipids. J Nutr. 1989;119(10):1376-82. PubMed PMID: 2685200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary fat composition on rat colon plasma membranes and fecal lipids. AU - Awad,A B, AU - Chattopadhyay,J P, AU - Danahy,M E, PY - 1989/10/1/pubmed PY - 1989/10/1/medline PY - 1989/10/1/entrez SP - 1376 EP - 82 JF - The Journal of nutrition JO - J. Nutr. VL - 119 IS - 10 N2 - The present study was designed to examine the effect of dietary fat composition on the structure of colon mucosal plasma membranes and fecal lipids. Rats were fed a purified diet containing 14% of either highly saturated fat (beef fat or butterfat) or highly polyunsaturated oil (safflower) in addition to 2% corn oil for 4 wk. Colon mucosal membranes were prepared and examined for lipid composition and protein pattern. Saturated fatty acid feeding resulted in the loss of some protein bands from plasma membranes compared to feeding polyunsaturated fatty acids. Within the saturated fatty acid--rich fats, feeding beef fat caused a greater loss than did feeding butterfat. Dietary fat composition had no effect on membrane content of phospholipid and cholesterol. Saturated fatty acid feeding resulted in an increase in the percentage of 18:1 in plasma membrane lipids compared to feeding safflower oil. The observed changes in the structure of colon mucosal membrane of animals fed the saturated fats were associated with an increase in fecal free fatty acids. There was a 4-fold and 2-fold increase in fecal free fatty acids with feeding the beef fat and butterfat diets, respectively, compared to the safflower oil diet. Alterations in fecal bile acid and free fatty acid composition were also noticed with feeding saturated fatty acids. The results obtained suggest that feeding saturated fatty acids as the main source of fat in the diet could influence the structure of colon mucosa, and this could be mediated through fecal free fatty acids. SN - 0022-3166 UR - https://www.unboundmedicine.com/medline/citation/2685200/Effect_of_dietary_fat_composition_on_rat_colon_plasma_membranes_and_fecal_lipids_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.1093/jn/119.10.1376 DB - PRIME DP - Unbound Medicine ER -