Tags

Type your tag names separated by a space and hit enter

High-density molecular characterization and association mapping in Ethiopian durum wheat landraces reveals high diversity and potential for wheat breeding.
Plant Biotechnol J. 2016 09; 14(9):1800-12.PB

Abstract

Durum wheat (Triticum turgidum subsp. durum) is a key crop worldwide, and yet, its improvement and adaptation to emerging environmental threats is made difficult by the limited amount of allelic variation included in its elite pool. New allelic diversity may provide novel loci to international crop breeding through quantitative trait loci (QTL) mapping in unexplored material. Here, we report the extensive molecular and phenotypic characterization of hundreds of Ethiopian durum wheat landraces and several Ethiopian improved lines. We test 81 587 markers scoring 30 155 single nucleotide polymorphisms and use them to survey the diversity, structure, and genome-specific variation in the panel. We show the uniqueness of Ethiopian germplasm using a siding collection of Mediterranean durum wheat accessions. We phenotype the Ethiopian panel for ten agronomic traits in two highly diversified Ethiopian environments for two consecutive years and use this information to conduct a genome-wide association study. We identify several loci underpinning agronomic traits of interest, both confirming loci already reported and describing new promising genomic regions. These loci may be efficiently targeted with molecular markers already available to conduct marker-assisted selection in Ethiopian and international wheat. We show that Ethiopian durum wheat represents an important and mostly unexplored source of durum wheat diversity. The panel analysed in this study allows the accumulation of QTL mapping experiments, providing the initial step for a quantitative, methodical exploitation of untapped diversity in producing a better wheat.

Authors+Show Affiliations

Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy. Department of Dryland Crop and Horticultural Sciences, Mekelle University, Mekelle, Ethiopia.Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy. Sirinka Agricultural Research Center, Sirinka, Woldia, Ethiopia.Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.Department of Agricultural Sciences, University of Bologna, Bologna, Italy.Bioversity International, C/O International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia.Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26853077

Citation

Mengistu, Dejene Kassahun, et al. "High-density Molecular Characterization and Association Mapping in Ethiopian Durum Wheat Landraces Reveals High Diversity and Potential for Wheat Breeding." Plant Biotechnology Journal, vol. 14, no. 9, 2016, pp. 1800-12.
Mengistu DK, Kidane YG, Catellani M, et al. High-density molecular characterization and association mapping in Ethiopian durum wheat landraces reveals high diversity and potential for wheat breeding. Plant Biotechnol J. 2016;14(9):1800-12.
Mengistu, D. K., Kidane, Y. G., Catellani, M., Frascaroli, E., Fadda, C., Pè, M. E., & Dell'Acqua, M. (2016). High-density molecular characterization and association mapping in Ethiopian durum wheat landraces reveals high diversity and potential for wheat breeding. Plant Biotechnology Journal, 14(9), 1800-12. https://doi.org/10.1111/pbi.12538
Mengistu DK, et al. High-density Molecular Characterization and Association Mapping in Ethiopian Durum Wheat Landraces Reveals High Diversity and Potential for Wheat Breeding. Plant Biotechnol J. 2016;14(9):1800-12. PubMed PMID: 26853077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-density molecular characterization and association mapping in Ethiopian durum wheat landraces reveals high diversity and potential for wheat breeding. AU - Mengistu,Dejene Kassahun, AU - Kidane,Yosef Gebrehawaryat, AU - Catellani,Marcello, AU - Frascaroli,Elisabetta, AU - Fadda,Carlo, AU - Pè,Mario Enrico, AU - Dell'Acqua,Matteo, Y1 - 2016/02/08/ PY - 2015/08/17/received PY - 2015/12/11/revised PY - 2016/01/06/accepted PY - 2016/2/9/entrez PY - 2016/2/9/pubmed PY - 2017/9/2/medline KW - Ethiopia KW - GWAS KW - Triticum turgidum subsp. durum KW - linkage disequilibrium KW - population genetics KW - quantitative trait loci SP - 1800 EP - 12 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 14 IS - 9 N2 - Durum wheat (Triticum turgidum subsp. durum) is a key crop worldwide, and yet, its improvement and adaptation to emerging environmental threats is made difficult by the limited amount of allelic variation included in its elite pool. New allelic diversity may provide novel loci to international crop breeding through quantitative trait loci (QTL) mapping in unexplored material. Here, we report the extensive molecular and phenotypic characterization of hundreds of Ethiopian durum wheat landraces and several Ethiopian improved lines. We test 81 587 markers scoring 30 155 single nucleotide polymorphisms and use them to survey the diversity, structure, and genome-specific variation in the panel. We show the uniqueness of Ethiopian germplasm using a siding collection of Mediterranean durum wheat accessions. We phenotype the Ethiopian panel for ten agronomic traits in two highly diversified Ethiopian environments for two consecutive years and use this information to conduct a genome-wide association study. We identify several loci underpinning agronomic traits of interest, both confirming loci already reported and describing new promising genomic regions. These loci may be efficiently targeted with molecular markers already available to conduct marker-assisted selection in Ethiopian and international wheat. We show that Ethiopian durum wheat represents an important and mostly unexplored source of durum wheat diversity. The panel analysed in this study allows the accumulation of QTL mapping experiments, providing the initial step for a quantitative, methodical exploitation of untapped diversity in producing a better wheat. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/26853077/High_density_molecular_characterization_and_association_mapping_in_Ethiopian_durum_wheat_landraces_reveals_high_diversity_and_potential_for_wheat_breeding_ L2 - https://doi.org/10.1111/pbi.12538 DB - PRIME DP - Unbound Medicine ER -