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Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism.

Abstract

The essential trace element selenium (Se) is required for thyroid hormone synthesis and metabolism. Selenoproteins contain selenocysteine and are responsible for biological functions of selenium. Glutathione peroxidase (GPx) is one of the major selenoproteins which protects the thyroid cells from oxidative damage. Selenoprotein P (SePP) is considered as the plasma selenium transporter to tissues. The aim of this study was to evaluate serum Se and SePP levels, and GPx activity in erythrocytes of children and adolescents with treated Hashimoto's thyroiditis, hypothyroidism, and normal subjects. Blood samples were collected from 32 patients with Hashimoto's thyroiditis, 20 with hypothyroidism, and 25 matched normal subjects. All the patients were under treatment with levothyroxine and at the time of analysis all of the thyroid function tests were normal. GPx enzyme activity was measured by spectrophotometry at 340 nm. Serum selenium levels were measured by high-resolution continuum source graphite furnace atomic absorption. SePP, TPOAb (anti-thyroid peroxidase antibody), and TgAb (anti-thyroglobulin antibody) were determined by ELISA kits. T4, T3, T3 uptake and TSH were also measured. Neither GPx activity nor SePP levels were significantly different in patients with Hashimoto's thyroiditis or hypothyroidism compared to normal subjects. Although GPx and SePP were both lower in patients with hypothyroidism compared to those with Hashimoto's thyroiditis and normal subjects but the difference was not significant. Serum Se levels also did not differ significantly in patients and normal subjects. We did not find any correlation between GPx or SePP with TPOAb or TgAb but SePP was significantly correlated with Se. Results show that in patients with Hashimoto's thyroiditis or hypothyroidism who have been under treatment with levothyroxine and have normal thyroid function tests, the GPx, SePP and Se levels are not significantly different.

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  • Authors+Show Affiliations

    ,

    Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Metabolic Disorders Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: nourbakhsh.m@iums.ac.ir.

    ,

    Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

    ,

    Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

    ,

    Department of Biochemistry, School of Medicine, Yazd University of Medical Sciences, Yazd, Iran.

    ,

    H.Aliasghar Hospital, Iran University of Medical Sciences, Tehran, Iran.

    ,

    Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

    ,

    Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

    ,

    Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

    Metabolic Disorders Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran; H.Aliasghar Hospital, Iran University of Medical Sciences, Tehran, Iran.

    Source

    MeSH

    Adolescent
    Child
    Female
    Glutathione Peroxidase
    Hashimoto Disease
    Humans
    Hypothyroidism
    Male
    Selenium
    Selenoprotein P
    Thyroid Gland

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    26854239

    Citation

    Nourbakhsh, Mitra, et al. "Selenium and Its Relationship With Selenoprotein P and Glutathione Peroxidase in Children and Adolescents With Hashimoto's Thyroiditis and Hypothyroidism." Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS), vol. 34, 2016, pp. 10-4.
    Nourbakhsh M, Ahmadpour F, Chahardoli B, et al. Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism. J Trace Elem Med Biol. 2016;34:10-4.
    Nourbakhsh, M., Ahmadpour, F., Chahardoli, B., Malekpour-Dehkordi, Z., Nourbakhsh, M., Hosseini-Fard, S. R., ... Razzaghy-Azar, M. (2016). Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism. Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS), 34, pp. 10-4. doi:10.1016/j.jtemb.2015.10.003.
    Nourbakhsh M, et al. Selenium and Its Relationship With Selenoprotein P and Glutathione Peroxidase in Children and Adolescents With Hashimoto's Thyroiditis and Hypothyroidism. J Trace Elem Med Biol. 2016;34:10-4. PubMed PMID: 26854239.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism. AU - Nourbakhsh,Mitra, AU - Ahmadpour,Fatemeh, AU - Chahardoli,Behnam, AU - Malekpour-Dehkordi,Zahra, AU - Nourbakhsh,Mona, AU - Hosseini-Fard,Seyed Reza, AU - Doustimotlagh,Amirhossein, AU - Golestani,Abolfazl, AU - Razzaghy-Azar,Maryam, Y1 - 2015/11/18/ PY - 2015/05/17/received PY - 2015/10/07/revised PY - 2015/10/22/accepted PY - 2016/2/9/entrez PY - 2016/2/9/pubmed PY - 2016/12/15/medline KW - Glutathione peroxidase KW - Hashimoto’s thyroiditis KW - Hypothyroidism KW - Selenium KW - Selenoprotein P KW - Thyroid SP - 10 EP - 4 JF - Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) JO - J Trace Elem Med Biol VL - 34 N2 - The essential trace element selenium (Se) is required for thyroid hormone synthesis and metabolism. Selenoproteins contain selenocysteine and are responsible for biological functions of selenium. Glutathione peroxidase (GPx) is one of the major selenoproteins which protects the thyroid cells from oxidative damage. Selenoprotein P (SePP) is considered as the plasma selenium transporter to tissues. The aim of this study was to evaluate serum Se and SePP levels, and GPx activity in erythrocytes of children and adolescents with treated Hashimoto's thyroiditis, hypothyroidism, and normal subjects. Blood samples were collected from 32 patients with Hashimoto's thyroiditis, 20 with hypothyroidism, and 25 matched normal subjects. All the patients were under treatment with levothyroxine and at the time of analysis all of the thyroid function tests were normal. GPx enzyme activity was measured by spectrophotometry at 340 nm. Serum selenium levels were measured by high-resolution continuum source graphite furnace atomic absorption. SePP, TPOAb (anti-thyroid peroxidase antibody), and TgAb (anti-thyroglobulin antibody) were determined by ELISA kits. T4, T3, T3 uptake and TSH were also measured. Neither GPx activity nor SePP levels were significantly different in patients with Hashimoto's thyroiditis or hypothyroidism compared to normal subjects. Although GPx and SePP were both lower in patients with hypothyroidism compared to those with Hashimoto's thyroiditis and normal subjects but the difference was not significant. Serum Se levels also did not differ significantly in patients and normal subjects. We did not find any correlation between GPx or SePP with TPOAb or TgAb but SePP was significantly correlated with Se. Results show that in patients with Hashimoto's thyroiditis or hypothyroidism who have been under treatment with levothyroxine and have normal thyroid function tests, the GPx, SePP and Se levels are not significantly different. SN - 1878-3252 UR - https://www.unboundmedicine.com/medline/citation/26854239/Selenium_and_its_relationship_with_selenoprotein_P_and_glutathione_peroxidase_in_children_and_adolescents_with_Hashimoto's_thyroiditis_and_hypothyroidism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0946-672X(15)30038-9 DB - PRIME DP - Unbound Medicine ER -