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Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.).
Genome. 2016 Mar; 59(3):159-65.G

Abstract

PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye Kustro, Imperial, and King II. A total of 427 and 387 markers were, respectively, located on individual chromosomes and chromosome arms of Kustro by using a set of wheat-rye monosomic addition lines and 13 monotelosomic addition lines, which were derived from T. aestivum L. 'Mianyang11' × S. cereale L. 'Kustro'. In addition, two sets of wheat-rye disomic addition lines, which were derived from T. aestivum L. var. Chinese Spring × S. cereale L. var. Imperial and T. aestivum L. 'Holdfast' × S. cereale L. var. King II, were used to test the chromosomal specificity of the 427 markers. The chromosomal locations of 281 markers were consistent among the three sets of wheat-rye addition lines. The markers developed in this study can be used to identify a given segment of rye chromosomes in wheat background and accelerate the utilization of elite genes on rye chromosomes in wheat breeding programs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Qiu L
a Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
Tang ZX
a Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China. b Institute of Ecological Agricultural, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
Li M
a Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
Fu SL
a Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.

MeSH

Chromosomes, PlantDNA, PlantGenetic MarkersPlant BreedingPolymerase Chain ReactionSecaleSequence Analysis, DNATriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26862664

Citation

Qiu, Ling, et al. "Development of New PCR-based Markers Specific for Chromosome Arms of Rye (Secale Cereale L.)." Genome, vol. 59, no. 3, 2016, pp. 159-65.
Qiu L, Tang ZX, Li M, et al. Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.). Genome. 2016;59(3):159-65.
Qiu, L., Tang, Z. X., Li, M., & Fu, S. L. (2016). Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.). Genome, 59(3), 159-65. https://doi.org/10.1139/gen-2015-0154
Qiu L, et al. Development of New PCR-based Markers Specific for Chromosome Arms of Rye (Secale Cereale L.). Genome. 2016;59(3):159-65. PubMed PMID: 26862664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.). AU - Qiu,Ling, AU - Tang,Zong-xiang, AU - Li,Meng, AU - Fu,Shu-lan, Y1 - 2016/02/10/ PY - 2016/2/11/entrez PY - 2016/2/11/pubmed PY - 2016/11/12/medline KW - PCR based KW - blé KW - chromosome-specific marker KW - marqueur PCR KW - marqueur spécifique d’un chromosome KW - rye KW - seigle KW - wheat SP - 159 EP - 65 JF - Genome JO - Genome VL - 59 IS - 3 N2 - PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye Kustro, Imperial, and King II. A total of 427 and 387 markers were, respectively, located on individual chromosomes and chromosome arms of Kustro by using a set of wheat-rye monosomic addition lines and 13 monotelosomic addition lines, which were derived from T. aestivum L. 'Mianyang11' × S. cereale L. 'Kustro'. In addition, two sets of wheat-rye disomic addition lines, which were derived from T. aestivum L. var. Chinese Spring × S. cereale L. var. Imperial and T. aestivum L. 'Holdfast' × S. cereale L. var. King II, were used to test the chromosomal specificity of the 427 markers. The chromosomal locations of 281 markers were consistent among the three sets of wheat-rye addition lines. The markers developed in this study can be used to identify a given segment of rye chromosomes in wheat background and accelerate the utilization of elite genes on rye chromosomes in wheat breeding programs. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/26862664/Development_of_new_PCR_based_markers_specific_for_chromosome_arms_of_rye__Secale_cereale_L___ DB - PRIME DP - Unbound Medicine ER -