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Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study.
Pharm Biol. 2016 Sep; 54(9):1671-9.PB

Abstract

Context Ethnopharmacological studies have demonstrated that plants of the Combretum genus presented antidiabetic activity, including Combretum lanceolatum Pohl ex Eichler (Combretaceae). Objective This study investigated the hepatic mechanisms of action of C. lanceolatum flowers ethanol extract (ClEtOH) related to its antihyperglycaemic effect in streptozotocin-diabetic rats. Materials and methods Male Wistar rats were divided into normal (N) and diabetic control (DC) rats treated with vehicle (water); diabetic rats treated with 500 mg/kg metformin (DMet) or 500 mg/kg ClEtOH (DT500). After 21 d of treatment, hepatic glucose and urea production were investigated through in situ perfused liver with l-glutamine. Changes in the phosphoenolpyruvate carboxykinase (PEPCK) levels and in the activation of adenosine monophosphate-activated protein kinase (AMPK) and insulin-signalling intermediates were also investigated. Results Similar to DMet, DT500 rats showed a reduction in the rates of hepatic production of glucose (46%) and urea (22%) in comparison with DC. This reduction was accompanied by a reduction in the PEPCK levels in liver of DT500 (28%) and DMet (43%) when compared with DC. AMPK phosphorylation levels were higher in the liver of DT500 (17%) and DMet (16%) rats. The basal AKT phosphorylation levels were increased in liver of DT500 rats, without differences in the insulin-stimulated AKT phosphorylation and in the insulin receptor levels between DC and DT500 rats. Discussion and conclusion The antidiabetic activity of ClEtOH can be attributed, at least in part, to inhibition of hepatic gluconeogenesis, probably due to the activation of both AMPK and AKT effectors and reduction in the PEPCK levels.

Authors+Show Affiliations

a Department of Chemistry , Federal University of Mato Grosso , Cuiabá , Mato Grosso , Brazil ;a Department of Chemistry , Federal University of Mato Grosso , Cuiabá , Mato Grosso , Brazil ;a Department of Chemistry , Federal University of Mato Grosso , Cuiabá , Mato Grosso , Brazil ;a Department of Chemistry , Federal University of Mato Grosso , Cuiabá , Mato Grosso , Brazil ;a Department of Chemistry , Federal University of Mato Grosso , Cuiabá , Mato Grosso , Brazil ;a Department of Chemistry , Federal University of Mato Grosso , Cuiabá , Mato Grosso , Brazil ;a Department of Chemistry , Federal University of Mato Grosso , Cuiabá , Mato Grosso , Brazil ;b Department of Physiological Sciences , State University of Londrina , Londrina , Parana , Brazil ;c Department of Clinical Analysis, School of Pharmaceutical Sciences , São Paulo State University, UNESP , Araraquara , São Paulo , Brazil.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

26864726

Citation

Siqueira, Juliany Torres, et al. "Combretum Lanceolatum Flowers Ethanol Extract Inhibits Hepatic Gluconeogenesis: an in Vivo Mechanism Study." Pharmaceutical Biology, vol. 54, no. 9, 2016, pp. 1671-9.
Siqueira JT, Batistela E, Pereira MP, et al. Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study. Pharm Biol. 2016;54(9):1671-9.
Siqueira, J. T., Batistela, E., Pereira, M. P., da Silva, V. C., de Sousa Junior, P. T., Andrade, C. M., Kawashita, N. H., Bertolini, G. L., & Baviera, A. M. (2016). Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study. Pharmaceutical Biology, 54(9), 1671-9. https://doi.org/10.3109/13880209.2015.1120321
Siqueira JT, et al. Combretum Lanceolatum Flowers Ethanol Extract Inhibits Hepatic Gluconeogenesis: an in Vivo Mechanism Study. Pharm Biol. 2016;54(9):1671-9. PubMed PMID: 26864726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study. AU - Siqueira,Juliany Torres, AU - Batistela,Emanuele, AU - Pereira,Mayara Peron, AU - da Silva,Virginia Claudia, AU - de Sousa Junior,Paulo Teixeira, AU - Andrade,Claudia Marlise Balbinotti, AU - Kawashita,Nair Honda, AU - Bertolini,Gisele Lopes, AU - Baviera,Amanda Martins, Y1 - 2016/02/10/ PY - 2016/2/12/entrez PY - 2016/2/13/pubmed PY - 2017/2/14/medline KW - Antidiabetic activity KW - glucose hepatic production KW - phosphoenolpyruvate carboxykinase SP - 1671 EP - 9 JF - Pharmaceutical biology JO - Pharm Biol VL - 54 IS - 9 N2 - Context Ethnopharmacological studies have demonstrated that plants of the Combretum genus presented antidiabetic activity, including Combretum lanceolatum Pohl ex Eichler (Combretaceae). Objective This study investigated the hepatic mechanisms of action of C. lanceolatum flowers ethanol extract (ClEtOH) related to its antihyperglycaemic effect in streptozotocin-diabetic rats. Materials and methods Male Wistar rats were divided into normal (N) and diabetic control (DC) rats treated with vehicle (water); diabetic rats treated with 500 mg/kg metformin (DMet) or 500 mg/kg ClEtOH (DT500). After 21 d of treatment, hepatic glucose and urea production were investigated through in situ perfused liver with l-glutamine. Changes in the phosphoenolpyruvate carboxykinase (PEPCK) levels and in the activation of adenosine monophosphate-activated protein kinase (AMPK) and insulin-signalling intermediates were also investigated. Results Similar to DMet, DT500 rats showed a reduction in the rates of hepatic production of glucose (46%) and urea (22%) in comparison with DC. This reduction was accompanied by a reduction in the PEPCK levels in liver of DT500 (28%) and DMet (43%) when compared with DC. AMPK phosphorylation levels were higher in the liver of DT500 (17%) and DMet (16%) rats. The basal AKT phosphorylation levels were increased in liver of DT500 rats, without differences in the insulin-stimulated AKT phosphorylation and in the insulin receptor levels between DC and DT500 rats. Discussion and conclusion The antidiabetic activity of ClEtOH can be attributed, at least in part, to inhibition of hepatic gluconeogenesis, probably due to the activation of both AMPK and AKT effectors and reduction in the PEPCK levels. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/26864726/Combretum_lanceolatum_flowers_ethanol_extract_inhibits_hepatic_gluconeogenesis:_an_in_vivo_mechanism_study_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2015.1120321 DB - PRIME DP - Unbound Medicine ER -