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Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil.
J Appl Microbiol. 2016 May; 120(5):1250-63.JA

Abstract

AIMS

In the present work the acidophilic actinobacteria strain was used as a novel reducing agent for the cheap, green and single-step synthesis of nanostructure silver particles. Structural, morphological and optical properties of the synthesized nanoparticles have been characterized by spectroscopy, dynamic light scattering and electron microscopy approach. The antimicrobial activity of silver nanoparticles against clinical strains such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis and Salmonella infantis alone and in combination with antibiotics were studied.

METHODS AND RESULTS

The crystalline and stable biosynthesized silver nanoparticles ranged in size from 4 to 45 nm and were mostly spherical in shape being characterized evolving several analytical techniques. The bioAgNPs inhibited growth of most bacterial strains. The highest antimicrobial activity was observed against Ps. aeruginosa (10 mm), followed by Staph. aureus, B. subtilis and Pr. mirabilis (all 8 mm). The lower activity was noticed for E. coli and Kl. pneumoniae (6 and 2 mm, respectively). Moreover, the synergistic effect of bio(AgNPs) with various commercially available antibiotics was also evaluated. The most significant results were observed for bio(AgNPs) combined with tetracycline, kanamycin, ampicillin and neomycin, followed by streptomycin and gentamycin against E. coli, Salm. infantis and Kl. pneumoniae. The most resistant bacteria to commercial antibiotics was Pr. mirabilis.

CONCLUSION

The Streptacidiphilus sp. strain CGG11n isolated from acidic soil can be used to efficiently synthesize the bioactive nanoparticles using inexpensive substances in an eco-friendly and nontoxic manner. The present work provides helpful insight into the development of new antimicrobial agents with the synergistic enhancement of the antibacterial mechanism against pathogenic micro-organisms.

SIGNIFICANCE AND IMPACT OF THE STUDY

The synthesized silver bionanoparticles from Streptacidiphilus sp. strain CGG11n possess potent inhibitory effect that offers valuable contribution to pharmaceutical associations.

Authors+Show Affiliations

Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26864807

Citation

Railean-Plugaru, V, et al. "Study of Silver Nanoparticles Synthesized By Acidophilic Strain of Actinobacteria Isolated From the of Picea Sitchensis Forest Soil." Journal of Applied Microbiology, vol. 120, no. 5, 2016, pp. 1250-63.
Railean-Plugaru V, Pomastowski P, Wypij M, et al. Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil. J Appl Microbiol. 2016;120(5):1250-63.
Railean-Plugaru, V., Pomastowski, P., Wypij, M., Szultka-Mlynska, M., Rafinska, K., Golinska, P., Dahm, H., & Buszewski, B. (2016). Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil. Journal of Applied Microbiology, 120(5), 1250-63. https://doi.org/10.1111/jam.13093
Railean-Plugaru V, et al. Study of Silver Nanoparticles Synthesized By Acidophilic Strain of Actinobacteria Isolated From the of Picea Sitchensis Forest Soil. J Appl Microbiol. 2016;120(5):1250-63. PubMed PMID: 26864807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil. AU - Railean-Plugaru,V, AU - Pomastowski,P, AU - Wypij,M, AU - Szultka-Mlynska,M, AU - Rafinska,K, AU - Golinska,P, AU - Dahm,H, AU - Buszewski,B, Y1 - 2016/04/04/ PY - 2015/07/27/received PY - 2015/10/23/revised PY - 2016/02/01/accepted PY - 2016/2/12/entrez PY - 2016/2/13/pubmed PY - 2017/1/10/medline KW - Actinomycetes KW - antibacterial activity KW - antibiotics KW - bioproducts KW - soil SP - 1250 EP - 63 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 120 IS - 5 N2 - AIMS: In the present work the acidophilic actinobacteria strain was used as a novel reducing agent for the cheap, green and single-step synthesis of nanostructure silver particles. Structural, morphological and optical properties of the synthesized nanoparticles have been characterized by spectroscopy, dynamic light scattering and electron microscopy approach. The antimicrobial activity of silver nanoparticles against clinical strains such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis and Salmonella infantis alone and in combination with antibiotics were studied. METHODS AND RESULTS: The crystalline and stable biosynthesized silver nanoparticles ranged in size from 4 to 45 nm and were mostly spherical in shape being characterized evolving several analytical techniques. The bioAgNPs inhibited growth of most bacterial strains. The highest antimicrobial activity was observed against Ps. aeruginosa (10 mm), followed by Staph. aureus, B. subtilis and Pr. mirabilis (all 8 mm). The lower activity was noticed for E. coli and Kl. pneumoniae (6 and 2 mm, respectively). Moreover, the synergistic effect of bio(AgNPs) with various commercially available antibiotics was also evaluated. The most significant results were observed for bio(AgNPs) combined with tetracycline, kanamycin, ampicillin and neomycin, followed by streptomycin and gentamycin against E. coli, Salm. infantis and Kl. pneumoniae. The most resistant bacteria to commercial antibiotics was Pr. mirabilis. CONCLUSION: The Streptacidiphilus sp. strain CGG11n isolated from acidic soil can be used to efficiently synthesize the bioactive nanoparticles using inexpensive substances in an eco-friendly and nontoxic manner. The present work provides helpful insight into the development of new antimicrobial agents with the synergistic enhancement of the antibacterial mechanism against pathogenic micro-organisms. SIGNIFICANCE AND IMPACT OF THE STUDY: The synthesized silver bionanoparticles from Streptacidiphilus sp. strain CGG11n possess potent inhibitory effect that offers valuable contribution to pharmaceutical associations. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/26864807/Study_of_silver_nanoparticles_synthesized_by_acidophilic_strain_of_Actinobacteria_isolated_from_the_of_Picea_sitchensis_forest_soil_ DB - PRIME DP - Unbound Medicine ER -