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Respiratory capacities of mitochondria of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 grown under glucose limitation.
Antonie Van Leeuwenhoek. 1989 Oct; 56(3):211-20.AV

Abstract

A comparative study was made of the in vitro respiratory capacity of mitochondria isolated from Saccharomyces cerevisiae and Candida utilis grown in glucose-limited chemostat cultures. An electron-microscopic analysis of whole cells revealed that the volume density of mitochondria was the same in both yeasts. Mitochondria from both organisms exhibited respiratory control with NADH, pyruvate + malate, 2-oxoglutarate + acetate or malate, and ethanol. The rate of oxidation of these compounds by isolated mitochondria was the same in both yeasts. The rate of oxidation of NADPH by mitochondria from S. cerevisiae was 10 times lower than by those from C. utilis. However, this low rate probably has no influence on the overall in vivo respiratory capacity of S. cerevisiae. The results are discussed in relation to the differences in metabolic behaviour between S. cerevisiae and C. utilis upon transition of cultures from glucose limitation to glucose excess. It is concluded that the occurrence of alcoholic fermentation in S. cerevisiae under these conditions does not result from a bottleneck in the respiratory capacity of the mitochondria.

Authors+Show Affiliations

Van Urk H
Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.
Bruinenberg PM
No affiliation info available
Veenhuis M
No affiliation info available
Scheffers WA
No affiliation info available
Van Dijken JP
No affiliation info available

MeSH

CandidaGlucoseMitochondriaOxidation-ReductionOxidoreductasesOxygenPolarographySaccharomyces cerevisiaeSubstrate Specificity

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2686549

Citation

Van Urk, H, et al. "Respiratory Capacities of Mitochondria of Saccharomyces Cerevisiae CBS 8066 and Candida Utilis CBS 621 Grown Under Glucose Limitation." Antonie Van Leeuwenhoek, vol. 56, no. 3, 1989, pp. 211-20.
Van Urk H, Bruinenberg PM, Veenhuis M, et al. Respiratory capacities of mitochondria of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 grown under glucose limitation. Antonie Van Leeuwenhoek. 1989;56(3):211-20.
Van Urk, H., Bruinenberg, P. M., Veenhuis, M., Scheffers, W. A., & Van Dijken, J. P. (1989). Respiratory capacities of mitochondria of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 grown under glucose limitation. Antonie Van Leeuwenhoek, 56(3), 211-20.
Van Urk H, et al. Respiratory Capacities of Mitochondria of Saccharomyces Cerevisiae CBS 8066 and Candida Utilis CBS 621 Grown Under Glucose Limitation. Antonie Van Leeuwenhoek. 1989;56(3):211-20. PubMed PMID: 2686549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Respiratory capacities of mitochondria of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 grown under glucose limitation. AU - Van Urk,H, AU - Bruinenberg,P M, AU - Veenhuis,M, AU - Scheffers,W A, AU - Van Dijken,J P, PY - 1989/10/1/pubmed PY - 1989/10/1/medline PY - 1989/10/1/entrez SP - 211 EP - 20 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 56 IS - 3 N2 - A comparative study was made of the in vitro respiratory capacity of mitochondria isolated from Saccharomyces cerevisiae and Candida utilis grown in glucose-limited chemostat cultures. An electron-microscopic analysis of whole cells revealed that the volume density of mitochondria was the same in both yeasts. Mitochondria from both organisms exhibited respiratory control with NADH, pyruvate + malate, 2-oxoglutarate + acetate or malate, and ethanol. The rate of oxidation of these compounds by isolated mitochondria was the same in both yeasts. The rate of oxidation of NADPH by mitochondria from S. cerevisiae was 10 times lower than by those from C. utilis. However, this low rate probably has no influence on the overall in vivo respiratory capacity of S. cerevisiae. The results are discussed in relation to the differences in metabolic behaviour between S. cerevisiae and C. utilis upon transition of cultures from glucose limitation to glucose excess. It is concluded that the occurrence of alcoholic fermentation in S. cerevisiae under these conditions does not result from a bottleneck in the respiratory capacity of the mitochondria. SN - 0003-6072 UR - https://www.unboundmedicine.com/medline/citation/2686549/Respiratory_capacities_of_mitochondria_of_Saccharomyces_cerevisiae_CBS_8066_and_Candida_utilis_CBS_621_grown_under_glucose_limitation_ DB - PRIME DP - Unbound Medicine ER -