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Free fatty acids, not triglycerides, are associated with non-alcoholic liver injury progression in high fat diet induced obese rats.
Lipids Health Dis. 2016 Feb 11; 15:27.LH

Abstract

BACKGROUND

The incidence of non-alcoholic fatty liver disease (NAFLD), commonly associated with obesity and metabolic syndrome, is increasing worldwide. However, the specific mechanisms that mediate the progression from simple steatosis to non-alcoholic steatohepatitis remain largely unclear. This study aimed to investigate the time dependent changes of triglyceride (TG) and free fatty acid (FFA) levels in the blood and liver over 24 weeks in high-fat diet-induced obese rats with NAFLD and to clarify the role of high FFA levels in the progression of liver injury.

METHODS

Male Wistar rats were randomly divided into three groups (n = 30 per group): the Control group, fed standard chow; the High-fat diet (HFD) group, fed high-fat chow; and the Acipimox group, fed an HFD plus acipimox (100 mg/kg/d, ig) for 8, 16 and 24 weeks. After treatment, blood and liver samples were collected for biochemical analyses, western blotting analysis and a histopathological study.

RESULTS

The visceral fat/weight and liver/body weight ratios were higher in both the HFD and Acipimox groups than in the Control group. The TG and FFA concentrations in blood and liver were increased in the HFD group and associated with elevated serum alanine aminotransferase (ALT) and liver malondialdehyde (MDA) levels and macro/microvesicular steatosis on hepatic fragments. Although the TG levels in the liver were similar between the HFD and Acipimox groups (p > 0.05), the FFA concentrations in the blood and liver were much lower in the latter group (p < 0.05). The Acipimox group showed normal ALT and MDA levels as well as less severe hepatic histological changes than did the HFD group (NAFLD activity score: 2.14 ± 0.14, 2.43 ± 0.20 and 2.63 ± 0.26 at 8, 16 and 24 weeks, respectively; p < 0.05 versus the HFD group at 24 weeks). The diacylglycerol acyltransferase 2 (DGAT2) protein levels were similar between the HFD and Acipimox groups (p > 0.05), but the protein expression level of carnitine palmitoyltransferase 1a (CPT-1a) was higher in the Acipimox group.

CONCLUSIONS

Liver TG accumulation does not cause cellular injury in the liver; rather, FFAs or their metabolites are responsible for liver injury via increased oxidative stress. It is suggested that the therapeutic efforts to prevent non-alcoholic liver injury progression should be focused on reducing the burden of fatty acids transported to the liver or those being synthesized in the liver.

Authors+Show Affiliations

Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Guoxue lane 37, Chengdu, Sichuan, 610041, China. jialiyysl@126.com.Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Guoxue lane 37, Chengdu, Sichuan, 610041, China. Hanlina333@yeah.net.Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Guoxue lane 37, Chengdu, Sichuan, 610041, China. zhuleilei2018@163.com.Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Guoxue lane 37, Chengdu, Sichuan, 610041, China. yerongyu@scu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26868515

Citation

Liu, Jiali, et al. "Free Fatty Acids, Not Triglycerides, Are Associated With Non-alcoholic Liver Injury Progression in High Fat Diet Induced Obese Rats." Lipids in Health and Disease, vol. 15, 2016, p. 27.
Liu J, Han L, Zhu L, et al. Free fatty acids, not triglycerides, are associated with non-alcoholic liver injury progression in high fat diet induced obese rats. Lipids Health Dis. 2016;15:27.
Liu, J., Han, L., Zhu, L., & Yu, Y. (2016). Free fatty acids, not triglycerides, are associated with non-alcoholic liver injury progression in high fat diet induced obese rats. Lipids in Health and Disease, 15, 27. https://doi.org/10.1186/s12944-016-0194-7
Liu J, et al. Free Fatty Acids, Not Triglycerides, Are Associated With Non-alcoholic Liver Injury Progression in High Fat Diet Induced Obese Rats. Lipids Health Dis. 2016 Feb 11;15:27. PubMed PMID: 26868515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Free fatty acids, not triglycerides, are associated with non-alcoholic liver injury progression in high fat diet induced obese rats. AU - Liu,Jiali, AU - Han,Lina, AU - Zhu,Leilei, AU - Yu,Yerong, Y1 - 2016/02/11/ PY - 2015/10/22/received PY - 2016/02/03/accepted PY - 2016/2/13/entrez PY - 2016/2/13/pubmed PY - 2016/12/15/medline SP - 27 EP - 27 JF - Lipids in health and disease JO - Lipids Health Dis VL - 15 N2 - BACKGROUND: The incidence of non-alcoholic fatty liver disease (NAFLD), commonly associated with obesity and metabolic syndrome, is increasing worldwide. However, the specific mechanisms that mediate the progression from simple steatosis to non-alcoholic steatohepatitis remain largely unclear. This study aimed to investigate the time dependent changes of triglyceride (TG) and free fatty acid (FFA) levels in the blood and liver over 24 weeks in high-fat diet-induced obese rats with NAFLD and to clarify the role of high FFA levels in the progression of liver injury. METHODS: Male Wistar rats were randomly divided into three groups (n = 30 per group): the Control group, fed standard chow; the High-fat diet (HFD) group, fed high-fat chow; and the Acipimox group, fed an HFD plus acipimox (100 mg/kg/d, ig) for 8, 16 and 24 weeks. After treatment, blood and liver samples were collected for biochemical analyses, western blotting analysis and a histopathological study. RESULTS: The visceral fat/weight and liver/body weight ratios were higher in both the HFD and Acipimox groups than in the Control group. The TG and FFA concentrations in blood and liver were increased in the HFD group and associated with elevated serum alanine aminotransferase (ALT) and liver malondialdehyde (MDA) levels and macro/microvesicular steatosis on hepatic fragments. Although the TG levels in the liver were similar between the HFD and Acipimox groups (p > 0.05), the FFA concentrations in the blood and liver were much lower in the latter group (p < 0.05). The Acipimox group showed normal ALT and MDA levels as well as less severe hepatic histological changes than did the HFD group (NAFLD activity score: 2.14 ± 0.14, 2.43 ± 0.20 and 2.63 ± 0.26 at 8, 16 and 24 weeks, respectively; p < 0.05 versus the HFD group at 24 weeks). The diacylglycerol acyltransferase 2 (DGAT2) protein levels were similar between the HFD and Acipimox groups (p > 0.05), but the protein expression level of carnitine palmitoyltransferase 1a (CPT-1a) was higher in the Acipimox group. CONCLUSIONS: Liver TG accumulation does not cause cellular injury in the liver; rather, FFAs or their metabolites are responsible for liver injury via increased oxidative stress. It is suggested that the therapeutic efforts to prevent non-alcoholic liver injury progression should be focused on reducing the burden of fatty acids transported to the liver or those being synthesized in the liver. SN - 1476-511X UR - https://www.unboundmedicine.com/medline/citation/26868515/Free_fatty_acids_not_triglycerides_are_associated_with_non_alcoholic_liver_injury_progression_in_high_fat_diet_induced_obese_rats_ L2 - https://lipidworld.biomedcentral.com/articles/10.1186/s12944-016-0194-7 DB - PRIME DP - Unbound Medicine ER -