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Biofilm production and antifungal susceptibility of co-cultured Malassezia pachydermatis and Candida parapsilosis isolated from canine seborrheic dermatitis.
Med Mycol. 2016 Jul 01; 54(5):544-9.MM

Abstract

The yeasts Malassezia (M.) pachydermatis and Candida (C.) parapsilosis are often co-isolated in case of canine seborrhea dermatitis (SD) and also are emerging as opportunistic pathogens of immunocompromised human beings. Increased information about how their relationship results in biofilm production and an antifungal response would be useful to inform treatment and control. This study was designed to investigate biofilm production derived from co-culture of M. pachydermatis and C. parapsilosis from dog skin and to determine their in vitro antifungal susceptibility. We demonstrated that regardless of yeast strain or origin all single and dual cultures produced biofilms within 24 hours, and the greatest amount was present after 72 hours. Biofilm production from mixed cultures was greater than for single strains (P < .05). All sessile forms of the single and dual cultures were resistant to the tested antifungals itraconazole and ketoconazole, whereas planktonic forms were susceptible. The study suggests that dual cultures produce stronger biofilms that are likely to enhance persistence in skin lesions in dogs and result in greater resistance to antifungal treatment.

Authors+Show Affiliations

Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330.Department of Medical Science, National Institute of Medical Science, Bangkok, 11000.Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330.Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand.Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330 Nuvee.P@chula.ac.th.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26868903

Citation

Bumroongthai, K, et al. "Biofilm Production and Antifungal Susceptibility of Co-cultured Malassezia Pachydermatis and Candida Parapsilosis Isolated From Canine Seborrheic Dermatitis." Medical Mycology, vol. 54, no. 5, 2016, pp. 544-9.
Bumroongthai K, Chetanachan P, Niyomtham W, et al. Biofilm production and antifungal susceptibility of co-cultured Malassezia pachydermatis and Candida parapsilosis isolated from canine seborrheic dermatitis. Med Mycol. 2016;54(5):544-9.
Bumroongthai, K., Chetanachan, P., Niyomtham, W., Yurayart, C., & Prapasarakul, N. (2016). Biofilm production and antifungal susceptibility of co-cultured Malassezia pachydermatis and Candida parapsilosis isolated from canine seborrheic dermatitis. Medical Mycology, 54(5), 544-9. https://doi.org/10.1093/mmy/myw002
Bumroongthai K, et al. Biofilm Production and Antifungal Susceptibility of Co-cultured Malassezia Pachydermatis and Candida Parapsilosis Isolated From Canine Seborrheic Dermatitis. Med Mycol. 2016 Jul 1;54(5):544-9. PubMed PMID: 26868903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biofilm production and antifungal susceptibility of co-cultured Malassezia pachydermatis and Candida parapsilosis isolated from canine seborrheic dermatitis. AU - Bumroongthai,K, AU - Chetanachan,P, AU - Niyomtham,W, AU - Yurayart,C, AU - Prapasarakul,N, Y1 - 2016/02/11/ PY - 2016/01/03/accepted PY - 2015/12/22/received PY - 2016/2/13/entrez PY - 2016/2/13/pubmed PY - 2017/3/21/medline KW - Candida parapsilosis KW - Malassezia pachydermatis KW - antifungal susceptibility KW - biofilm production KW - dogs SP - 544 EP - 9 JF - Medical mycology JO - Med. Mycol. VL - 54 IS - 5 N2 - The yeasts Malassezia (M.) pachydermatis and Candida (C.) parapsilosis are often co-isolated in case of canine seborrhea dermatitis (SD) and also are emerging as opportunistic pathogens of immunocompromised human beings. Increased information about how their relationship results in biofilm production and an antifungal response would be useful to inform treatment and control. This study was designed to investigate biofilm production derived from co-culture of M. pachydermatis and C. parapsilosis from dog skin and to determine their in vitro antifungal susceptibility. We demonstrated that regardless of yeast strain or origin all single and dual cultures produced biofilms within 24 hours, and the greatest amount was present after 72 hours. Biofilm production from mixed cultures was greater than for single strains (P < .05). All sessile forms of the single and dual cultures were resistant to the tested antifungals itraconazole and ketoconazole, whereas planktonic forms were susceptible. The study suggests that dual cultures produce stronger biofilms that are likely to enhance persistence in skin lesions in dogs and result in greater resistance to antifungal treatment. SN - 1460-2709 UR - https://www.unboundmedicine.com/medline/citation/26868903/Biofilm_production_and_antifungal_susceptibility_of_co_cultured_Malassezia_pachydermatis_and_Candida_parapsilosis_isolated_from_canine_seborrheic_dermatitis_ L2 - https://academic.oup.com/mmy/article-lookup/doi/10.1093/mmy/myw002 DB - PRIME DP - Unbound Medicine ER -