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Regulation of laryngeal squamous cell cancer progression by the lncRNA H19/miR-148a-3p/DNMT1 axis.
Oncotarget 2016; 7(10):11553-66O

Abstract

Laryngeal squamous cell carcinoma (LSCC) is a highly aggressive malignant cancer. The regulation of LSCC progression by long non-coding RNA (lncRNA) was not well understood. In this study, we reported that the lncRNA H19 was upregulated in LSCC. The expression levels of H19 were inversely correlated with the survival rate of LSCC patients. Knockdown of H19 expression inhibited LSCC cell migration, invasion and proliferation. We identified microRNA miR-148a-3p as an inhibitory target for H19. Overexpression of miR-148a-3p reduced LSCC migration, invasion and proliferation cell, while inhibition of miR-148a-3p did the opposite. The inhibition of LSCC progression induced by H19 knockdown required the activity of miR-148a-3p. We also identified DNA methyltransferase enzyme DNMT1 as a target of miR-148a-3p. Cellular DNA methylation levels were inhibited by both miR-148a-3p overexpression and H19 knockdown. In summary, our study demonstrated that the lncRNA H19 promoted LSCC progression via miR-148a-3p and DNMT1.

Authors+Show Affiliations

Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Head and Neck Surgery, The Oncology Hospital of Jilin province, Changchun, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Orthopaedic Surgery and The Orthopaedic Hospital Research Center, University of California, Los Angeles, California, USA.Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26872375

Citation

Wu, Tianyi, et al. "Regulation of Laryngeal Squamous Cell Cancer Progression By the lncRNA H19/miR-148a-3p/DNMT1 Axis." Oncotarget, vol. 7, no. 10, 2016, pp. 11553-66.
Wu T, Qu L, He G, et al. Regulation of laryngeal squamous cell cancer progression by the lncRNA H19/miR-148a-3p/DNMT1 axis. Oncotarget. 2016;7(10):11553-66.
Wu, T., Qu, L., He, G., Tian, L., Li, L., Zhou, H., ... Sun, Y. (2016). Regulation of laryngeal squamous cell cancer progression by the lncRNA H19/miR-148a-3p/DNMT1 axis. Oncotarget, 7(10), pp. 11553-66. doi:10.18632/oncotarget.7270.
Wu T, et al. Regulation of Laryngeal Squamous Cell Cancer Progression By the lncRNA H19/miR-148a-3p/DNMT1 Axis. Oncotarget. 2016 Mar 8;7(10):11553-66. PubMed PMID: 26872375.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of laryngeal squamous cell cancer progression by the lncRNA H19/miR-148a-3p/DNMT1 axis. AU - Wu,Tianyi, AU - Qu,Lingmei, AU - He,Guoqing, AU - Tian,Linli, AU - Li,Liang, AU - Zhou,Han, AU - Jin,Qian, AU - Ren,Jingyuan, AU - Wang,Yu, AU - Wang,Jingting, AU - Kan,Xuan, AU - Liu,Ming, AU - Shen,Jia, AU - Guo,Mian, AU - Sun,Yanan, PY - 2015/07/07/received PY - 2015/12/05/accepted PY - 2016/2/13/entrez PY - 2016/2/13/pubmed PY - 2017/12/2/medline KW - DNMT1 KW - laryngeal squamous cell cancer KW - lncRNA H19 KW - miR-148a-3p SP - 11553 EP - 66 JF - Oncotarget JO - Oncotarget VL - 7 IS - 10 N2 - Laryngeal squamous cell carcinoma (LSCC) is a highly aggressive malignant cancer. The regulation of LSCC progression by long non-coding RNA (lncRNA) was not well understood. In this study, we reported that the lncRNA H19 was upregulated in LSCC. The expression levels of H19 were inversely correlated with the survival rate of LSCC patients. Knockdown of H19 expression inhibited LSCC cell migration, invasion and proliferation. We identified microRNA miR-148a-3p as an inhibitory target for H19. Overexpression of miR-148a-3p reduced LSCC migration, invasion and proliferation cell, while inhibition of miR-148a-3p did the opposite. The inhibition of LSCC progression induced by H19 knockdown required the activity of miR-148a-3p. We also identified DNA methyltransferase enzyme DNMT1 as a target of miR-148a-3p. Cellular DNA methylation levels were inhibited by both miR-148a-3p overexpression and H19 knockdown. In summary, our study demonstrated that the lncRNA H19 promoted LSCC progression via miR-148a-3p and DNMT1. SN - 1949-2553 UR - https://www.unboundmedicine.com/medline/citation/26872375/Regulation_of_laryngeal_squamous_cell_cancer_progression_by_the_lncRNA_H19/miR_148a_3p/DNMT1_axis_ L2 - http://www.impactjournals.com/oncotarget/misc/linkedout.php?pii=7270 DB - PRIME DP - Unbound Medicine ER -