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Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros).
Insect Biochem Mol Biol. 2016 Apr; 71:58-71.IB

Abstract

RNA interference (RNAi) is a gene silencing mechanism that is present in animals and plants and is triggered by double stranded RNA (dsRNA) or small interfering RNA (siRNA), depending on the organism. In the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), RNAi can be achieved by feeding rootworms dsRNA added to artificial diet or plant tissues transformed to express dsRNA. The effect of RNAi depends on the targeted gene function and can range from an absence of phenotypic response to readily apparent responses, including lethality. Furthermore, RNAi can directly affect individuals that consume dsRNA or the effect may be transferred to the next generation. Our previous work described the potential use of genes involved in embryonic development as a parental RNAi technology for the control of WCR. In this study, we describe the use of chromatin-remodeling ATPases as target genes to achieve parental gene silencing in two insect pests, a coleopteran, WCR, and a hemipteran, the Neotropical brown stink bug, Euschistus heros Fabricius (Hemiptera: Pentatomidae). Our results show that dsRNA targeting chromatin-remodeling ATPase transcripts, brahma, mi-2, and iswi strongly reduced the fecundity of the exposed females in both insect species. Additionally, knockdown of chd1 reduced the fecundity of E. heros.

Authors+Show Affiliations

Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.Entomology and Nematology Department, Charles Steinmetz Hall, University of Florida, P.O. Box 110620, Gainesville, FL 32611-0620, United States. Electronic address: bsiegfried1@ufl.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26873291

Citation

Fishilevich, Elane, et al. "Use of Chromatin Remodeling ATPases as RNAi Targets for Parental Control of Western Corn Rootworm (Diabrotica Virgifera Virgifera) and Neotropical Brown Stink Bug (Euschistus Heros)." Insect Biochemistry and Molecular Biology, vol. 71, 2016, pp. 58-71.
Fishilevich E, Vélez AM, Khajuria C, et al. Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros). Insect Biochem Mol Biol. 2016;71:58-71.
Fishilevich, E., Vélez, A. M., Khajuria, C., Frey, M. L., Hamm, R. L., Wang, H., Schulenberg, G. A., Bowling, A. J., Pence, H. E., Gandra, P., Arora, K., Storer, N. P., Narva, K. E., & Siegfried, B. D. (2016). Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros). Insect Biochemistry and Molecular Biology, 71, 58-71. https://doi.org/10.1016/j.ibmb.2016.02.004
Fishilevich E, et al. Use of Chromatin Remodeling ATPases as RNAi Targets for Parental Control of Western Corn Rootworm (Diabrotica Virgifera Virgifera) and Neotropical Brown Stink Bug (Euschistus Heros). Insect Biochem Mol Biol. 2016;71:58-71. PubMed PMID: 26873291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros). AU - Fishilevich,Elane, AU - Vélez,Ana M, AU - Khajuria,Chitvan, AU - Frey,Meghan L F, AU - Hamm,Ronda L, AU - Wang,Haichuan, AU - Schulenberg,Greg A, AU - Bowling,Andrew J, AU - Pence,Heather E, AU - Gandra,Premchand, AU - Arora,Kanika, AU - Storer,Nicholas P, AU - Narva,Kenneth E, AU - Siegfried,Blair D, Y1 - 2016/02/10/ PY - 2015/10/08/received PY - 2016/02/04/revised PY - 2016/02/04/accepted PY - 2016/2/14/entrez PY - 2016/2/14/pubmed PY - 2016/12/15/medline KW - Brahma KW - Chd1 KW - Chromatin-remodeling ATPase KW - Diabrotica KW - Iswi KW - Mi-2 KW - Parental RNAi KW - Stink bug SP - 58 EP - 71 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 71 N2 - RNA interference (RNAi) is a gene silencing mechanism that is present in animals and plants and is triggered by double stranded RNA (dsRNA) or small interfering RNA (siRNA), depending on the organism. In the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), RNAi can be achieved by feeding rootworms dsRNA added to artificial diet or plant tissues transformed to express dsRNA. The effect of RNAi depends on the targeted gene function and can range from an absence of phenotypic response to readily apparent responses, including lethality. Furthermore, RNAi can directly affect individuals that consume dsRNA or the effect may be transferred to the next generation. Our previous work described the potential use of genes involved in embryonic development as a parental RNAi technology for the control of WCR. In this study, we describe the use of chromatin-remodeling ATPases as target genes to achieve parental gene silencing in two insect pests, a coleopteran, WCR, and a hemipteran, the Neotropical brown stink bug, Euschistus heros Fabricius (Hemiptera: Pentatomidae). Our results show that dsRNA targeting chromatin-remodeling ATPase transcripts, brahma, mi-2, and iswi strongly reduced the fecundity of the exposed females in both insect species. Additionally, knockdown of chd1 reduced the fecundity of E. heros. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/26873291/Use_of_chromatin_remodeling_ATPases_as_RNAi_targets_for_parental_control_of_western_corn_rootworm__Diabrotica_virgifera_virgifera__and_Neotropical_brown_stink_bug__Euschistus_heros__ DB - PRIME DP - Unbound Medicine ER -