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[Expression and significance of AKT/mTOR signaling pathway in natural degeneration in vitro model of endplate chondrocytes of rats].
Zhonghua Yi Xue Za Zhi 2016; 96(5):375-9ZY

Abstract

OBJECTIVE

To investigate the expression and significance of AKT/mTOR signaling pathway in natural degeneration in vitro model of rat endplate chondrocytes.

METHODS

Rat endplate chondrocytes were isolated and cultured, and the natural degeneration in vitro model of rat endplate chondrocytes was established. The endplate chondrocytes were divided into blank control group (P2 cells), natural degeneration passage group (P5 cells), AKT/mTOR signaling pathway inhibitor group (P5 cells), AKT/mTOR signaling pathway agonist group (P5 cells). The endplate chondrocyte morphology changes were observed under inverted microscope; Toluidine blue staining was used to detect the expression changes of proteoglycans in endplate chondrocytes; Western blot was used to detect the expression changes of AKT and P-AKT; real-time polymerase chain reaction was used to detect the expression of type Ⅱ collagen, proteoglycan, SOX9 and the key gene of AKT/mTOR signaling pathway, mTOR gene.

RESULTS

With in vitro passage of rat endplate chondrocytes, phenotypes of rat endplate chondrocytes gradually reduced or even lost; the type Ⅱ collagen (P5/P2=0.28, P=0.042 7), proteoglycan (P5/P2=0.33, P=0.026 3), SOX9 (P5/P2=0.40, P=0.018 2) and mTOR (P5/P2=0.28, P=0.038 1) expression were significantly reduced in natural degeneration passage group; in AKT/mTOR signaling pathway inhibitor group: type Ⅱ collagen (P5/P2=0.19, P=0.034 7), proteoglycan (P5/P2=0.25, P=0.023), SOX9 (P5/P2=0.31, P=0.034 2) and mTOR (P5/P2=0.20, P=0.024 1) expression were decreased compared with natural passage group; in AKT/mTOR signaling pathway agonist group: type Ⅱ collagen (P5/P2=0.41, P=0.044 1), proteoglycan (P5/P2=0.53, P=0.015 1), SOX9 (P5/P2=0.61, P=0.019 7) and mTOR (P5/P2=0.41, P=0.038 1) expression were increased compared with natural passage group and inhibitor group.

CONCLUSION

AKT/mTOR signaling pathway may play an important role in the degenerative process of rat endplate chondrocytes in vitro, artificial control or intervention of AKT/mTOR signaling pathway may accelerate, inhibit or slow the degeneration of endplate cartilage of rats.

Authors+Show Affiliations

Department of Spine Surgery, the First Affiliated Hospital of Wannan Medical College, Wuhu 241000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26875719

Citation

Zhang, Shufeng, et al. "[Expression and Significance of AKT/mTOR Signaling Pathway in Natural Degeneration in Vitro Model of Endplate Chondrocytes of Rats]." Zhonghua Yi Xue Za Zhi, vol. 96, no. 5, 2016, pp. 375-9.
Zhang S, Wang H, Zhang T, et al. [Expression and significance of AKT/mTOR signaling pathway in natural degeneration in vitro model of endplate chondrocytes of rats]. Zhonghua Yi Xue Za Zhi. 2016;96(5):375-9.
Zhang, S., Wang, H., Zhang, T., Xu, H., Ma, M., & Zhang, X. (2016). [Expression and significance of AKT/mTOR signaling pathway in natural degeneration in vitro model of endplate chondrocytes of rats]. Zhonghua Yi Xue Za Zhi, 96(5), pp. 375-9. doi:10.3760/cma.j.issn.0376-2491.2016.05.013.
Zhang S, et al. [Expression and Significance of AKT/mTOR Signaling Pathway in Natural Degeneration in Vitro Model of Endplate Chondrocytes of Rats]. Zhonghua Yi Xue Za Zhi. 2016 Feb 2;96(5):375-9. PubMed PMID: 26875719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression and significance of AKT/mTOR signaling pathway in natural degeneration in vitro model of endplate chondrocytes of rats]. AU - Zhang,Shufeng, AU - Wang,Hong, AU - Zhang,Tao, AU - Xu,Hongguang, AU - Ma,Mingming, AU - Zhang,Xiaoling, PY - 2016/2/16/entrez PY - 2016/2/16/pubmed PY - 2016/5/26/medline SP - 375 EP - 9 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 96 IS - 5 N2 - OBJECTIVE: To investigate the expression and significance of AKT/mTOR signaling pathway in natural degeneration in vitro model of rat endplate chondrocytes. METHODS: Rat endplate chondrocytes were isolated and cultured, and the natural degeneration in vitro model of rat endplate chondrocytes was established. The endplate chondrocytes were divided into blank control group (P2 cells), natural degeneration passage group (P5 cells), AKT/mTOR signaling pathway inhibitor group (P5 cells), AKT/mTOR signaling pathway agonist group (P5 cells). The endplate chondrocyte morphology changes were observed under inverted microscope; Toluidine blue staining was used to detect the expression changes of proteoglycans in endplate chondrocytes; Western blot was used to detect the expression changes of AKT and P-AKT; real-time polymerase chain reaction was used to detect the expression of type Ⅱ collagen, proteoglycan, SOX9 and the key gene of AKT/mTOR signaling pathway, mTOR gene. RESULTS: With in vitro passage of rat endplate chondrocytes, phenotypes of rat endplate chondrocytes gradually reduced or even lost; the type Ⅱ collagen (P5/P2=0.28, P=0.042 7), proteoglycan (P5/P2=0.33, P=0.026 3), SOX9 (P5/P2=0.40, P=0.018 2) and mTOR (P5/P2=0.28, P=0.038 1) expression were significantly reduced in natural degeneration passage group; in AKT/mTOR signaling pathway inhibitor group: type Ⅱ collagen (P5/P2=0.19, P=0.034 7), proteoglycan (P5/P2=0.25, P=0.023), SOX9 (P5/P2=0.31, P=0.034 2) and mTOR (P5/P2=0.20, P=0.024 1) expression were decreased compared with natural passage group; in AKT/mTOR signaling pathway agonist group: type Ⅱ collagen (P5/P2=0.41, P=0.044 1), proteoglycan (P5/P2=0.53, P=0.015 1), SOX9 (P5/P2=0.61, P=0.019 7) and mTOR (P5/P2=0.41, P=0.038 1) expression were increased compared with natural passage group and inhibitor group. CONCLUSION: AKT/mTOR signaling pathway may play an important role in the degenerative process of rat endplate chondrocytes in vitro, artificial control or intervention of AKT/mTOR signaling pathway may accelerate, inhibit or slow the degeneration of endplate cartilage of rats. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/26875719/[Expression_and_significance_of_AKT/mTOR_signaling_pathway_in_natural_degeneration_in_vitro_model_of_endplate_chondrocytes_of_rats]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=2016&vol=96&issue=5&fpage=375 DB - PRIME DP - Unbound Medicine ER -