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Gum tragacanth/poly(l-lactic acid) nanofibrous scaffolds for application in regeneration of peripheral nerve damage.
Carbohydr Polym 2016; 140:104-12CP

Abstract

Nanofibrous nerve guides have gained huge interest in supporting the peripheral nerve regeneration due to their abilities to simulate the topography, mechanical, biological and extracellular matrix morphology of native tissue. Gum tragacanth (GT) is a biocompatible mixture of polysaccharides that has been used in biomedical applications. During this study, we fabricated aligned and random nanofibers from poly(l-lactic acid) and gum tragacanth (PLLA/GT) in various ratios (100:0, 75:25, and 50:50) by electrospinning. Scanning electron microscope demonstrated smooth and uniform nanofibers with diameters in the range of 733±65nm and 226±73nm for align PLLA and random PLLA/GT 50:50 nanofibers, respectively. FTIR analysis, contact angle, in vitro biodegradation and tensile measurements were carried out to evaluate the chemical and mechanical properties of the different scaffolds. PLLA/GT 75:25 exhibited the most balanced properties compared to other scaffolds and was used for in vitro culture of nerve cells (PC12) to assess the potential of using these scaffolds as a substrate for nerve regeneration. The cells were found to attach and proliferate on aligned PLLA/GT 75:25 scaffolds, expressing bi-polar neurite extensions and the orientation of nerve cells was along the direction of the fiber alignment. Results of 8 days of in vitro culture of PC12 cells on aligned PLLA/GT 75:25 nanofibers, showed 20% increase in cell proliferation compared to PLLA/GT 75:25 random nanofibers. PLLA/GT 75:25 aligned nanofibers acted as a favorable cue to support neurite outgrowth and nerve cell elongation compared with PLLA nanofibers. Our results showed that aligned PLLA/GT 75:25 nanofibers are promising substrates for application as bioengineered grafts for nerve tissue regeneration.

Authors+Show Affiliations

Textile Group, Engineering Department, Bonab University, Bonab, Iran.Department of Mechanical Engineering, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore.Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran. Electronic address: hajirb@aut.ac.ir.Department of Mechanical Engineering, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26876833

Citation

Ranjbar-Mohammadi, Marziyeh, et al. "Gum Tragacanth/poly(l-lactic Acid) Nanofibrous Scaffolds for Application in Regeneration of Peripheral Nerve Damage." Carbohydrate Polymers, vol. 140, 2016, pp. 104-12.
Ranjbar-Mohammadi M, Prabhakaran MP, Bahrami SH, et al. Gum tragacanth/poly(l-lactic acid) nanofibrous scaffolds for application in regeneration of peripheral nerve damage. Carbohydr Polym. 2016;140:104-12.
Ranjbar-Mohammadi, M., Prabhakaran, M. P., Bahrami, S. H., & Ramakrishna, S. (2016). Gum tragacanth/poly(l-lactic acid) nanofibrous scaffolds for application in regeneration of peripheral nerve damage. Carbohydrate Polymers, 140, pp. 104-12. doi:10.1016/j.carbpol.2015.12.012.
Ranjbar-Mohammadi M, et al. Gum Tragacanth/poly(l-lactic Acid) Nanofibrous Scaffolds for Application in Regeneration of Peripheral Nerve Damage. Carbohydr Polym. 2016 Apr 20;140:104-12. PubMed PMID: 26876833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gum tragacanth/poly(l-lactic acid) nanofibrous scaffolds for application in regeneration of peripheral nerve damage. AU - Ranjbar-Mohammadi,Marziyeh, AU - Prabhakaran,Molamma P, AU - Bahrami,S Hajir, AU - Ramakrishna,Seeram, Y1 - 2015/12/12/ PY - 2015/09/06/received PY - 2015/11/19/revised PY - 2015/12/08/accepted PY - 2016/2/16/entrez PY - 2016/2/16/pubmed PY - 2016/12/15/medline KW - Align nanofibers KW - Gum tragacanth KW - Nerve regeneration KW - Poly(l-lactic acid) SP - 104 EP - 12 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 140 N2 - Nanofibrous nerve guides have gained huge interest in supporting the peripheral nerve regeneration due to their abilities to simulate the topography, mechanical, biological and extracellular matrix morphology of native tissue. Gum tragacanth (GT) is a biocompatible mixture of polysaccharides that has been used in biomedical applications. During this study, we fabricated aligned and random nanofibers from poly(l-lactic acid) and gum tragacanth (PLLA/GT) in various ratios (100:0, 75:25, and 50:50) by electrospinning. Scanning electron microscope demonstrated smooth and uniform nanofibers with diameters in the range of 733±65nm and 226±73nm for align PLLA and random PLLA/GT 50:50 nanofibers, respectively. FTIR analysis, contact angle, in vitro biodegradation and tensile measurements were carried out to evaluate the chemical and mechanical properties of the different scaffolds. PLLA/GT 75:25 exhibited the most balanced properties compared to other scaffolds and was used for in vitro culture of nerve cells (PC12) to assess the potential of using these scaffolds as a substrate for nerve regeneration. The cells were found to attach and proliferate on aligned PLLA/GT 75:25 scaffolds, expressing bi-polar neurite extensions and the orientation of nerve cells was along the direction of the fiber alignment. Results of 8 days of in vitro culture of PC12 cells on aligned PLLA/GT 75:25 nanofibers, showed 20% increase in cell proliferation compared to PLLA/GT 75:25 random nanofibers. PLLA/GT 75:25 aligned nanofibers acted as a favorable cue to support neurite outgrowth and nerve cell elongation compared with PLLA nanofibers. Our results showed that aligned PLLA/GT 75:25 nanofibers are promising substrates for application as bioengineered grafts for nerve tissue regeneration. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/26876833/Gum_tragacanth/poly_l_lactic_acid__nanofibrous_scaffolds_for_application_in_regeneration_of_peripheral_nerve_damage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0144-8617(15)01190-X DB - PRIME DP - Unbound Medicine ER -