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The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing.
Elife. 2016 Feb 02; 5E

Abstract

A common strategy by which bacterial pathogens reside in humans is by shifting from a virulent lifestyle, (systemic infection), to a dormant carrier state. Two major serovars of Salmonella enterica, Typhi and Typhimurium, have evolved a two-component regulatory system to exist inside Salmonella-containing vacuoles in the macrophage, as well as to persist as asymptomatic biofilms in the gallbladder. Here we present evidence that SsrB, a transcriptional regulator encoded on the SPI-2 pathogenicity-island, determines the switch between these two lifestyles by controlling ancestral and horizontally-acquired genes. In the acidic macrophage vacuole, the kinase SsrA phosphorylates SsrB, and SsrB~P relieves silencing of virulence genes and activates their transcription. In the absence of SsrA, unphosphorylated SsrB directs transcription of factors required for biofilm formation specifically by activating csgD (agfD), the master biofilm regulator by disrupting the silenced, H-NS-bound promoter. Anti-silencing mechanisms thus control the switch between opposing lifestyles.

Authors+Show Affiliations

Mechanobiology Institute, National University of Singapore, Singapore, Singapore.Mechanobiology Institute, National University of Singapore, Singapore, Singapore. Department of Physics, National University of Singapore, Singapore, Singapore.Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.Nanoscience and Nanotechnology Institute, National University of Singapore, Singapore, Singapore. Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore.Mechanobiology Institute, National University of Singapore, Singapore, Singapore. Department of Physics, National University of Singapore, Singapore, Singapore.Mechanobiology Institute, National University of Singapore, Singapore, Singapore. Jesse Brown Veterans Affairs Medical Center, University of Illinois-Chicago, Chicago, United States. Department of Microbiology and Immunology, University of Illinois-Chicago, Chicago, United States.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26880544

Citation

Desai, Stuti K., et al. "The Horizontally-acquired Response Regulator SsrB Drives a Salmonella Lifestyle Switch By Relieving Biofilm Silencing." ELife, vol. 5, 2016.
Desai SK, Winardhi RS, Periasamy S, et al. The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing. Elife. 2016;5.
Desai, S. K., Winardhi, R. S., Periasamy, S., Dykas, M. M., Jie, Y., & Kenney, L. J. (2016). The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing. ELife, 5. https://doi.org/10.7554/eLife.10747
Desai SK, et al. The Horizontally-acquired Response Regulator SsrB Drives a Salmonella Lifestyle Switch By Relieving Biofilm Silencing. Elife. 2016 Feb 2;5 PubMed PMID: 26880544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing. AU - Desai,Stuti K, AU - Winardhi,Ricksen S, AU - Periasamy,Saravanan, AU - Dykas,Michal M, AU - Jie,Yan, AU - Kenney,Linda J, Y1 - 2016/02/02/ PY - 2015/08/09/received PY - 2016/01/06/accepted PY - 2016/2/17/entrez PY - 2016/2/18/pubmed PY - 2016/10/27/medline KW - CsgD/AgfD KW - H-NS KW - Salmonella Typhimurium KW - SsrA/B KW - biofilm KW - infectious disease KW - microbiology KW - two-component regulatory system JF - eLife JO - Elife VL - 5 N2 - A common strategy by which bacterial pathogens reside in humans is by shifting from a virulent lifestyle, (systemic infection), to a dormant carrier state. Two major serovars of Salmonella enterica, Typhi and Typhimurium, have evolved a two-component regulatory system to exist inside Salmonella-containing vacuoles in the macrophage, as well as to persist as asymptomatic biofilms in the gallbladder. Here we present evidence that SsrB, a transcriptional regulator encoded on the SPI-2 pathogenicity-island, determines the switch between these two lifestyles by controlling ancestral and horizontally-acquired genes. In the acidic macrophage vacuole, the kinase SsrA phosphorylates SsrB, and SsrB~P relieves silencing of virulence genes and activates their transcription. In the absence of SsrA, unphosphorylated SsrB directs transcription of factors required for biofilm formation specifically by activating csgD (agfD), the master biofilm regulator by disrupting the silenced, H-NS-bound promoter. Anti-silencing mechanisms thus control the switch between opposing lifestyles. SN - 2050-084X UR - https://www.unboundmedicine.com/medline/citation/26880544/The_horizontally_acquired_response_regulator_SsrB_drives_a_Salmonella_lifestyle_switch_by_relieving_biofilm_silencing_ L2 - https://doi.org/10.7554/eLife.10747 DB - PRIME DP - Unbound Medicine ER -