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Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety.
Funct Integr Genomics. 2016 May; 16(3):269-79.FI

Abstract

Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lee JY
National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea. jy0820@korea.kr.
Beom HR
National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea.
Altenbach SB
USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA, 94710, USA.
Lim SH
National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea.
Kim YT
Department of Bioresource Sciences, Andong National University, Andong, 760-749, Korea.
Kang CS
National Institute of Crop Science, RDA, Jeonju, 565-851, Korea.
Yoon UH
National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea.
Gupta R
Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Korea.
Kim ST
Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Korea.
Ahn SN
Department of Agronomy, Chungnam National University, Daejeon, 357-764, Korea.
Kim YM
National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea. ymikim@korea.kr.

MeSH

AllelesAmino Acid SequenceBreadElectrophoresis, Gel, Two-DimensionalGlutensHaplotypesMolecular WeightPseudogenesTandem Mass SpectrometryTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26882917

Citation

Lee, Jong-Yeol, et al. "Comprehensive Identification of LMW-GS Genes and Their Protein Products in a Common Wheat Variety." Functional & Integrative Genomics, vol. 16, no. 3, 2016, pp. 269-79.
Lee JY, Beom HR, Altenbach SB, et al. Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. Funct Integr Genomics. 2016;16(3):269-79.
Lee, J. Y., Beom, H. R., Altenbach, S. B., Lim, S. H., Kim, Y. T., Kang, C. S., Yoon, U. H., Gupta, R., Kim, S. T., Ahn, S. N., & Kim, Y. M. (2016). Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. Functional & Integrative Genomics, 16(3), 269-79. https://doi.org/10.1007/s10142-016-0482-3
Lee JY, et al. Comprehensive Identification of LMW-GS Genes and Their Protein Products in a Common Wheat Variety. Funct Integr Genomics. 2016;16(3):269-79. PubMed PMID: 26882917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. AU - Lee,Jong-Yeol, AU - Beom,Hye-Rang, AU - Altenbach,Susan B, AU - Lim,Sun-Hyung, AU - Kim,Yeong-Tae, AU - Kang,Chon-Sik, AU - Yoon,Ung-Han, AU - Gupta,Ravi, AU - Kim,Sun-Tae, AU - Ahn,Sang-Nag, AU - Kim,Young-Mi, Y1 - 2016/02/16/ PY - 2015/08/31/received PY - 2016/02/02/accepted PY - 2016/01/24/revised PY - 2016/2/18/entrez PY - 2016/2/18/pubmed PY - 2017/2/9/medline KW - LMW-GS genes KW - Proteomics KW - Triticum aestivum L. KW - Wheat quality improvement SP - 269 EP - 79 JF - Functional & integrative genomics JO - Funct Integr Genomics VL - 16 IS - 3 N2 - Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour. SN - 1438-7948 UR - https://www.unboundmedicine.com/medline/citation/26882917/Comprehensive_identification_of_LMW_GS_genes_and_their_protein_products_in_a_common_wheat_variety_ DB - PRIME DP - Unbound Medicine ER -