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Cloning of TaSST genes associated with water soluble carbohydrate content in bread wheat stems and development of a functional marker.
Theor Appl Genet. 2016 May; 129(5):1061-70.TA

Abstract

KEY MESSAGE

We cloned TaSST genes, developed a gene-specific marker for TaSST-D1, and identified three QTL in the Doumai/Shi 4185 RIL population. TaSST-D1 is within one of the three QTL. Sucrose:sucrose-1-fructosyltransferase (1-SST), a critical enzyme in the fructan biosynthetic pathway, is significantly and positively associated with water soluble carbohydrate (WSC) content in bread wheat stems. In the present study, wheat 1-SST genes (TaSST) were isolated and located on chromosomes 4A, 7A and 7D. Sequence analysis of TaSST-D1 revealed 15 single nucleotide polymorphisms (SNP) in the third exon between cultivars with higher and lower WSC content. A cleaved amplified polymorphism sequence (CAPS) marker, WSC7D, based on the polymorphism at position 1216 (C-G) was developed to discriminate the two alleles. WSC7D was located on chromosome 7DS using a recombinant inbred line (RIL) population from a Doumai/Shi 4185 cross, and a set of Chinese Spring nullisomic-tetrasomic lines. TaSST-D1 co-segregated with the CAPS marker WSC7D and was linked to SNP marker BS00108793_51 on chromosome 7DS at a genetic distance of 6.1 cM. It explained 8.8, 10.9, and 11.3% of the phenotypic variances in trials at Beijing and Shijiazhuang as well as the averaged data from those environments, respectively. Two additional QTL (QWSC.caas-4BS and QWSC.caas-7AS) besides TaSST-D1 were mapped in the RIL population. One hundred and forty-nine Chinese wheat cultivars and advanced lines tested in four environments were used to validate a highly significant (P < 0.01) association between WSC7D and WSC content in wheat stems. WSC7D can be used as a gene-specific marker for improvement of stem WSC content in wheat breeding programs.

Authors+Show Affiliations

National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.Cotton Research Institute, CAAS, Huanghedadao, Anyang, Henan, China.National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, 830052, Urumqi, Xinjiang, China.National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China. xiaxianchun@caas.cn.National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, 100081, Beijing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26883047

Citation

Dong, Yan, et al. "Cloning of TaSST Genes Associated With Water Soluble Carbohydrate Content in Bread Wheat Stems and Development of a Functional Marker." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 129, no. 5, 2016, pp. 1061-70.
Dong Y, Zhang Y, Xiao Y, et al. Cloning of TaSST genes associated with water soluble carbohydrate content in bread wheat stems and development of a functional marker. Theor Appl Genet. 2016;129(5):1061-70.
Dong, Y., Zhang, Y., Xiao, Y., Yan, J., Liu, J., Wen, W., Zhang, Y., Jing, R., Xia, X., & He, Z. (2016). Cloning of TaSST genes associated with water soluble carbohydrate content in bread wheat stems and development of a functional marker. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 129(5), 1061-70. https://doi.org/10.1007/s00122-016-2683-5
Dong Y, et al. Cloning of TaSST Genes Associated With Water Soluble Carbohydrate Content in Bread Wheat Stems and Development of a Functional Marker. Theor Appl Genet. 2016;129(5):1061-70. PubMed PMID: 26883047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning of TaSST genes associated with water soluble carbohydrate content in bread wheat stems and development of a functional marker. AU - Dong,Yan, AU - Zhang,Yan, AU - Xiao,Yonggui, AU - Yan,Jun, AU - Liu,Jindong, AU - Wen,Weie, AU - Zhang,Yong, AU - Jing,Ruilian, AU - Xia,Xianchun, AU - He,Zhonghu, Y1 - 2016/02/16/ PY - 2015/10/22/received PY - 2016/01/23/accepted PY - 2016/2/18/entrez PY - 2016/2/18/pubmed PY - 2016/9/7/medline SP - 1061 EP - 70 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 129 IS - 5 N2 - KEY MESSAGE: We cloned TaSST genes, developed a gene-specific marker for TaSST-D1, and identified three QTL in the Doumai/Shi 4185 RIL population. TaSST-D1 is within one of the three QTL. Sucrose:sucrose-1-fructosyltransferase (1-SST), a critical enzyme in the fructan biosynthetic pathway, is significantly and positively associated with water soluble carbohydrate (WSC) content in bread wheat stems. In the present study, wheat 1-SST genes (TaSST) were isolated and located on chromosomes 4A, 7A and 7D. Sequence analysis of TaSST-D1 revealed 15 single nucleotide polymorphisms (SNP) in the third exon between cultivars with higher and lower WSC content. A cleaved amplified polymorphism sequence (CAPS) marker, WSC7D, based on the polymorphism at position 1216 (C-G) was developed to discriminate the two alleles. WSC7D was located on chromosome 7DS using a recombinant inbred line (RIL) population from a Doumai/Shi 4185 cross, and a set of Chinese Spring nullisomic-tetrasomic lines. TaSST-D1 co-segregated with the CAPS marker WSC7D and was linked to SNP marker BS00108793_51 on chromosome 7DS at a genetic distance of 6.1 cM. It explained 8.8, 10.9, and 11.3% of the phenotypic variances in trials at Beijing and Shijiazhuang as well as the averaged data from those environments, respectively. Two additional QTL (QWSC.caas-4BS and QWSC.caas-7AS) besides TaSST-D1 were mapped in the RIL population. One hundred and forty-nine Chinese wheat cultivars and advanced lines tested in four environments were used to validate a highly significant (P < 0.01) association between WSC7D and WSC content in wheat stems. WSC7D can be used as a gene-specific marker for improvement of stem WSC content in wheat breeding programs. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/26883047/Cloning_of_TaSST_genes_associated_with_water_soluble_carbohydrate_content_in_bread_wheat_stems_and_development_of_a_functional_marker_ L2 - https://dx.doi.org/10.1007/s00122-016-2683-5 DB - PRIME DP - Unbound Medicine ER -