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Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering.
AAPS PharmSciTech 2017; 18(1):72-81AP

Abstract

In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.

Authors+Show Affiliations

Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, 686560, India. kajal.ghosal@gmail.com. Dr. B. C. Roy College of Pharmacy and Allied Health Sciences, Durgapur, 713206, India. kajal.ghosal@gmail.com.Plasma Technologies, Central European Institute of Technology, Masaryk University, Brno, 61137, Czech Republic.Plasma Technologies, Central European Institute of Technology, Masaryk University, Brno, 61137, Czech Republic.Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, 686560, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26883261

Citation

Ghosal, Kajal, et al. "Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering." AAPS PharmSciTech, vol. 18, no. 1, 2017, pp. 72-81.
Ghosal K, Manakhov A, Zajíčková L, et al. Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering. AAPS PharmSciTech. 2017;18(1):72-81.
Ghosal, K., Manakhov, A., Zajíčková, L., & Thomas, S. (2017). Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering. AAPS PharmSciTech, 18(1), pp. 72-81. doi:10.1208/s12249-016-0500-8.
Ghosal K, et al. Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering. AAPS PharmSciTech. 2017 01 1;18(1):72-81. PubMed PMID: 26883261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering. AU - Ghosal,Kajal, AU - Manakhov,Anton, AU - Zajíčková,Lenka, AU - Thomas,Sabu, Y1 - 2016/02/16/ PY - 2015/10/28/received PY - 2016/02/04/accepted PY - 2016/2/18/pubmed PY - 2017/1/18/medline PY - 2016/2/18/entrez KW - PCL KW - compatibility study KW - composites KW - electrospinning KW - skin tissue engineering SP - 72 EP - 81 JF - AAPS PharmSciTech JO - AAPS PharmSciTech VL - 18 IS - 1 N2 - In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing. SN - 1530-9932 UR - https://www.unboundmedicine.com/medline/citation/26883261/Structural_and_Surface_Compatibility_Study_of_Modified_Electrospun_Poly_ε_caprolactone___PCL__Composites_for_Skin_Tissue_Engineering_ L2 - https://dx.doi.org/10.1208/s12249-016-0500-8 DB - PRIME DP - Unbound Medicine ER -