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Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells.
Toxicol In Vitro. 2016 Jun; 33:9-15.TV

Abstract

The ethanolic extract of propolis, especially the Brazilian green type, is widely and mainly used for therapeutic purposes despite the lack of knowledge about its effects and its cellular mode of action. This type of propolis, derived from Baccharis dracunculifolia (alecrim-do-campo), has been extensively commercialized and the consumers use it to enhance health. This work aimed to assess the genotoxic/mutagenic and antigenotoxic/antimutagenic potentials of the ethanolic extracts of Brazilian green propolis and of B. dracunculifolia, on mammalian cells. It was not observed genotoxic and mutagenic effects by both extracts. After evaluate the exposure of the cells to each extract with a recognized mutagen, simultaneously, the results showed a significant reduction on DNA damage. The experiment carried out with a pre-incubation period was more effective than without incubation test, showing that the tested extracts were able to inactivate the mutagen before it could react with the DNA.

Authors+Show Affiliations

Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil. Electronic address: mmr@rc.unesp.br.Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, UFES - Universidade Federal do Espírito Santo, Avenida Fernando Ferrari, 514, Goiabeiras, 29075-910 Vitória, ES, Brazil.Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, UFES - Universidade Federal do Espírito Santo, Avenida Marechal Campos, 1468, Maruípe, 29040-090 Vitória, ES, Brazil.Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil.Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil. Electronic address: mamm@rc.unesp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26891814

Citation

Roberto, Matheus Mantuanelli, et al. "Evaluation of the Genotoxicity/mutagenicity and Antigenotoxicity/antimutagenicity Induced By Propolis and Baccharis Dracunculifolia, By in Vitro Study With HTC Cells." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 33, 2016, pp. 9-15.
Roberto MM, Matsumoto ST, Jamal CM, et al. Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells. Toxicol In Vitro. 2016;33:9-15.
Roberto, M. M., Matsumoto, S. T., Jamal, C. M., Malaspina, O., & Marin-Morales, M. A. (2016). Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 33, 9-15. https://doi.org/10.1016/j.tiv.2016.02.005
Roberto MM, et al. Evaluation of the Genotoxicity/mutagenicity and Antigenotoxicity/antimutagenicity Induced By Propolis and Baccharis Dracunculifolia, By in Vitro Study With HTC Cells. Toxicol In Vitro. 2016;33:9-15. PubMed PMID: 26891814.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells. AU - Roberto,Matheus Mantuanelli, AU - Matsumoto,Sílvia Tamie, AU - Jamal,Cláudia Masrouah, AU - Malaspina,Osmar, AU - Marin-Morales,Maria Aparecida, Y1 - 2016/02/16/ PY - 2015/09/18/received PY - 2016/02/11/revised PY - 2016/02/13/accepted PY - 2016/2/20/entrez PY - 2016/2/20/pubmed PY - 2017/1/7/medline KW - Brazilian green propolis KW - Caffeic acid (PubChem CID: 689043) KW - Comet assay KW - Cytokinesis-blockage micronucleus test KW - Ethanolic extract KW - Ferulic acid (PubChem CID: 445858) KW - Kaempferol (PubChem CID: 5280863) KW - Methyl methanesulfonate (PubChem CID: 4156) KW - Mutagen inactivation KW - Quercetin (PubChem CID: 5280343) KW - Rutin (PubChem CID: 5280805) SP - 9 EP - 15 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 33 N2 - The ethanolic extract of propolis, especially the Brazilian green type, is widely and mainly used for therapeutic purposes despite the lack of knowledge about its effects and its cellular mode of action. This type of propolis, derived from Baccharis dracunculifolia (alecrim-do-campo), has been extensively commercialized and the consumers use it to enhance health. This work aimed to assess the genotoxic/mutagenic and antigenotoxic/antimutagenic potentials of the ethanolic extracts of Brazilian green propolis and of B. dracunculifolia, on mammalian cells. It was not observed genotoxic and mutagenic effects by both extracts. After evaluate the exposure of the cells to each extract with a recognized mutagen, simultaneously, the results showed a significant reduction on DNA damage. The experiment carried out with a pre-incubation period was more effective than without incubation test, showing that the tested extracts were able to inactivate the mutagen before it could react with the DNA. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/26891814/Evaluation_of_the_genotoxicity/mutagenicity_and_antigenotoxicity/antimutagenicity_induced_by_propolis_and_Baccharis_dracunculifolia_by_in_vitro_study_with_HTC_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(16)30020-0 DB - PRIME DP - Unbound Medicine ER -