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Molecular cloning of plane pollen allergen Pla a 3 and its utility as diagnostic marker for peach associated plane pollen allergy.
Clin Exp Allergy. 2016 05; 46(5):764-74.CE

Abstract

BACKGROUND

Non-specific lipid transfer proteins (nsLTP) are considered to provoke allergic symptoms to plane tree pollen, which are frequently associated with peach allergy.

OBJECTIVE

The objective was to clone the cDNA of plane pollen nsLTP Pla a 3, to characterize IgE-binding and allergenic potency of recombinant Pla a 3 in comparison to its natural counterpart and peach nsLTP Pru p 3.

METHODS

Natural Pla a 3 was purified from plane pollen and analysed by mass spectrometry (MS). Recombinant Pla a 3 was characterized by SDS-PAGE and CD spectroscopy. Specific IgE to extract, components of plane pollen and Pru p 3 was measured by ImmunoCAP in sera of patients allergic to either plane pollen (n = 10), peach (n = 15) or both (n = 15). Biological potency of the proteins was investigated by in vitro mediator release assays and IgE cross-reactivity by competitive ELISA.

RESULTS

Two Pla a 3 isoforms were identified. Recombinant Pla a 3 showed high purity, structural integrity, IgE-binding capacity comparable to nPla a 3 and biological potency. Sensitization to plane pollen extract was confirmed in 24/25 plane pollen allergics. The frequency of sensitization to Pla a 3 was 53% among patients allergic to both plane pollen and peach and 10% among plane pollen allergics tolerating peach where most patients were sensitized to Pla a 1. Pla a 3 and Pru p 3 showed strong bi-directional IgE cross-reactivity in patients allergic to peach and plane pollen, but not in peach allergics tolerating plane pollen. Levels of IgE-binding were generally higher to Pru p 3 than to Pla a 3.

CONCLUSION

Sensitization to Pla a 3 is relevant in a subgroup of plane pollen allergics with concomitant peach allergy. IgE testing with Pla a 3 may serve as a marker to identify plane pollen allergic patients at risk of LTP-mediated food reactions and thereby improve in vitro diagnostic procedures.

Authors+Show Affiliations

VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.ThermoFisher Scientific, Uppsala, Sweden.VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.Allergy Unit, Pneumology Department, Hospital Clinic, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.Test and Therapy Allergens, Paul-Ehrlich-Institut, Langen, Germany.VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.Allergy Unit, Pneumology Department, Hospital Clinic, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.ThermoFisher Scientific, Uppsala, Sweden.VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26892183

Citation

Wangorsch, A, et al. "Molecular Cloning of Plane Pollen Allergen Pla a 3 and Its Utility as Diagnostic Marker for Peach Associated Plane Pollen Allergy." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 46, no. 5, 2016, pp. 764-74.
Wangorsch A, Larsson H, Messmer M, et al. Molecular cloning of plane pollen allergen Pla a 3 and its utility as diagnostic marker for peach associated plane pollen allergy. Clin Exp Allergy. 2016;46(5):764-74.
Wangorsch, A., Larsson, H., Messmer, M., García-Moral, A., Lauer, I., Wolfheimer, S., Schülke, S., Bartra, J., Vieths, S., Lidholm, J., & Scheurer, S. (2016). Molecular cloning of plane pollen allergen Pla a 3 and its utility as diagnostic marker for peach associated plane pollen allergy. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 46(5), 764-74. https://doi.org/10.1111/cea.12721
Wangorsch A, et al. Molecular Cloning of Plane Pollen Allergen Pla a 3 and Its Utility as Diagnostic Marker for Peach Associated Plane Pollen Allergy. Clin Exp Allergy. 2016;46(5):764-74. PubMed PMID: 26892183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning of plane pollen allergen Pla a 3 and its utility as diagnostic marker for peach associated plane pollen allergy. AU - Wangorsch,A, AU - Larsson,H, AU - Messmer,M, AU - García-Moral,A, AU - Lauer,I, AU - Wolfheimer,S, AU - Schülke,S, AU - Bartra,J, AU - Vieths,S, AU - Lidholm,J, AU - Scheurer,S, PY - 2015/07/19/received PY - 2016/01/22/revised PY - 2016/02/07/accepted PY - 2016/2/20/entrez PY - 2016/2/20/pubmed PY - 2017/12/23/medline KW - LTP syndrome KW - Platanus allergy KW - Pla a 3 KW - Pru p 3 KW - peach allergy KW - plane pollen SP - 764 EP - 74 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 46 IS - 5 N2 - BACKGROUND: Non-specific lipid transfer proteins (nsLTP) are considered to provoke allergic symptoms to plane tree pollen, which are frequently associated with peach allergy. OBJECTIVE: The objective was to clone the cDNA of plane pollen nsLTP Pla a 3, to characterize IgE-binding and allergenic potency of recombinant Pla a 3 in comparison to its natural counterpart and peach nsLTP Pru p 3. METHODS: Natural Pla a 3 was purified from plane pollen and analysed by mass spectrometry (MS). Recombinant Pla a 3 was characterized by SDS-PAGE and CD spectroscopy. Specific IgE to extract, components of plane pollen and Pru p 3 was measured by ImmunoCAP in sera of patients allergic to either plane pollen (n = 10), peach (n = 15) or both (n = 15). Biological potency of the proteins was investigated by in vitro mediator release assays and IgE cross-reactivity by competitive ELISA. RESULTS: Two Pla a 3 isoforms were identified. Recombinant Pla a 3 showed high purity, structural integrity, IgE-binding capacity comparable to nPla a 3 and biological potency. Sensitization to plane pollen extract was confirmed in 24/25 plane pollen allergics. The frequency of sensitization to Pla a 3 was 53% among patients allergic to both plane pollen and peach and 10% among plane pollen allergics tolerating peach where most patients were sensitized to Pla a 1. Pla a 3 and Pru p 3 showed strong bi-directional IgE cross-reactivity in patients allergic to peach and plane pollen, but not in peach allergics tolerating plane pollen. Levels of IgE-binding were generally higher to Pru p 3 than to Pla a 3. CONCLUSION: Sensitization to Pla a 3 is relevant in a subgroup of plane pollen allergics with concomitant peach allergy. IgE testing with Pla a 3 may serve as a marker to identify plane pollen allergic patients at risk of LTP-mediated food reactions and thereby improve in vitro diagnostic procedures. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/26892183/Molecular_cloning_of_plane_pollen_allergen_Pla_a_3_and_its_utility_as_diagnostic_marker_for_peach_associated_plane_pollen_allergy_ L2 - https://doi.org/10.1111/cea.12721 DB - PRIME DP - Unbound Medicine ER -