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Effect of biochar amendment on the control of soil sulfonamides, antibiotic-resistant bacteria, and gene enrichment in lettuce tissues.
J Hazard Mater. 2016 May 15; 309:219-27.JH

Abstract

Considering the potential threat of vegetables growing in antibiotic-polluted soil with high abundance of antibiotic-resistant genes (ARGs) against human health through the food chain, it is thus urgent to develop novel control technology to ensure vegetable safety. In the present work, pot experiments were conducted in lettuce cultivation to assess the impedance effect of biochar amendment on soil sulfonamides (SAs), antibiotic-resistant bacteria (ARB), and ARG enrichment in lettuce tissues. After 100 days of cultivation, lettuce cultivation with biochar amendment exhibited the greatest soil SA dissipation as well as the significant improvement of lettuce growth indices, with residual soil SAs mainly existing as the tightly bound fraction. Moreover, the SA contents in roots and new/old leaves were reduced by one to two orders of magnitude compared to those without biochar amendment. In addition, isolate counts for SA-resistant bacterial endophytes in old leaves and sul gene abundances in roots and old leaves also decreased significantly after biochar application. However, neither SA resistant bacteria nor sul genes were detected in new leaves. It was the first study to demonstrate that biochar amendment can be a practical strategy to protect lettuce safety growing in SA-polluted soil with rich ARB and ARGs.

Authors+Show Affiliations

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China.Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China. Electronic address: fengyanfang@163.com.Nanjing Institute of Environmental Science, Ministry of Environmental Protection of China, Nanjing 210042, PR China.Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.Collaborative Innovation Center of Advanced Microstructures, Jiangsu Provincial Key Laboratory of Photonic and Electronic Materials, School of Electronic Science and Engineering, Nanjing University, Nanjing 210093, PR China.Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China. Electronic address: Jiangxin@issas.ac.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26896719

Citation

Ye, Mao, et al. "Effect of Biochar Amendment On the Control of Soil Sulfonamides, Antibiotic-resistant Bacteria, and Gene Enrichment in Lettuce Tissues." Journal of Hazardous Materials, vol. 309, 2016, pp. 219-27.
Ye M, Sun M, Feng Y, et al. Effect of biochar amendment on the control of soil sulfonamides, antibiotic-resistant bacteria, and gene enrichment in lettuce tissues. J Hazard Mater. 2016;309:219-27.
Ye, M., Sun, M., Feng, Y., Wan, J., Xie, S., Tian, D., Zhao, Y., Wu, J., Hu, F., Li, H., & Jiang, X. (2016). Effect of biochar amendment on the control of soil sulfonamides, antibiotic-resistant bacteria, and gene enrichment in lettuce tissues. Journal of Hazardous Materials, 309, 219-27. https://doi.org/10.1016/j.jhazmat.2015.10.074
Ye M, et al. Effect of Biochar Amendment On the Control of Soil Sulfonamides, Antibiotic-resistant Bacteria, and Gene Enrichment in Lettuce Tissues. J Hazard Mater. 2016 May 15;309:219-27. PubMed PMID: 26896719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of biochar amendment on the control of soil sulfonamides, antibiotic-resistant bacteria, and gene enrichment in lettuce tissues. AU - Ye,Mao, AU - Sun,Mingming, AU - Feng,Yanfang, AU - Wan,Jinzhong, AU - Xie,Shanni, AU - Tian,Da, AU - Zhao,Yu, AU - Wu,Jun, AU - Hu,Feng, AU - Li,Huixin, AU - Jiang,Xin, Y1 - 2015/11/04/ PY - 2015/07/30/received PY - 2015/10/25/revised PY - 2015/10/31/accepted PY - 2016/2/21/entrez PY - 2016/2/21/pubmed PY - 2016/12/15/medline KW - ARB KW - ARGs KW - Biochar addition KW - Impedance effect KW - Lettuce cultivation SP - 219 EP - 27 JF - Journal of hazardous materials JO - J Hazard Mater VL - 309 N2 - Considering the potential threat of vegetables growing in antibiotic-polluted soil with high abundance of antibiotic-resistant genes (ARGs) against human health through the food chain, it is thus urgent to develop novel control technology to ensure vegetable safety. In the present work, pot experiments were conducted in lettuce cultivation to assess the impedance effect of biochar amendment on soil sulfonamides (SAs), antibiotic-resistant bacteria (ARB), and ARG enrichment in lettuce tissues. After 100 days of cultivation, lettuce cultivation with biochar amendment exhibited the greatest soil SA dissipation as well as the significant improvement of lettuce growth indices, with residual soil SAs mainly existing as the tightly bound fraction. Moreover, the SA contents in roots and new/old leaves were reduced by one to two orders of magnitude compared to those without biochar amendment. In addition, isolate counts for SA-resistant bacterial endophytes in old leaves and sul gene abundances in roots and old leaves also decreased significantly after biochar application. However, neither SA resistant bacteria nor sul genes were detected in new leaves. It was the first study to demonstrate that biochar amendment can be a practical strategy to protect lettuce safety growing in SA-polluted soil with rich ARB and ARGs. SN - 1873-3336 UR - https://www.unboundmedicine.com/medline/citation/26896719/Effect_of_biochar_amendment_on_the_control_of_soil_sulfonamides_antibiotic_resistant_bacteria_and_gene_enrichment_in_lettuce_tissues_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3894(15)30200-4 DB - PRIME DP - Unbound Medicine ER -