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Identification of steroleosin in oil bodies of pine megagametophytes.
Plant Physiol Biochem. 2016 Apr; 101:173-181.PP

Abstract

Three classes of integral proteins termed oleosin, caleosin and steroleosin have been identified in seed oil bodies of diverse angiosperm species. Recently, two oleosin isoforms and one caleosin were identified in megagametophyte oil bodies of pine (Pinus massoniana), a representative gymnosperm species. In this study, a putative steroleosin of approximately 41 kDa was observed in isolated oil bodies of pine megagametophytes, and its corresponding cDNA fragment was obtained by PCR cloning and further confirmed by mass spectrometric analysis. Phylogenetic tree analysis showed that pine steroleosin was evolutionarily more closely-related to steroleosin-B than steroleosin-A found in angiosperm seed oil bodies. As expected, artificial oil bodies constituted with recombinant steroleosin over-expressed in Escherichia coli were less stable and larger than native pine oil bodies. Filipin staining of artificial oil bodies sheltered by recombinant steroleosin with or without its sterol binding domain showed that the sterol binding domain was responsible for the sterol binding capability of steroleosin. Sterol-coupling dehydrogenase activity was demonstrated in artificial oil bodies constituted with recombinant steroleosin as well as in purified pine oil bodies.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung 944, Taiwan.Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung 944, Taiwan. Electronic address: villy@nmmba.gov.tw.Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan. Electronic address: TCTZEN@dragon.nchu.edu.tw.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26897709

Citation

Pasaribu, Buntora, et al. "Identification of Steroleosin in Oil Bodies of Pine Megagametophytes." Plant Physiology and Biochemistry : PPB, vol. 101, 2016, pp. 173-181.
Pasaribu B, Chung TY, Chen CS, et al. Identification of steroleosin in oil bodies of pine megagametophytes. Plant Physiol Biochem. 2016;101:173-181.
Pasaribu, B., Chung, T. Y., Chen, C. S., Jiang, P. L., & Tzen, J. T. C. (2016). Identification of steroleosin in oil bodies of pine megagametophytes. Plant Physiology and Biochemistry : PPB, 101, 173-181. https://doi.org/10.1016/j.plaphy.2016.02.008
Pasaribu B, et al. Identification of Steroleosin in Oil Bodies of Pine Megagametophytes. Plant Physiol Biochem. 2016;101:173-181. PubMed PMID: 26897709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of steroleosin in oil bodies of pine megagametophytes. AU - Pasaribu,Buntora, AU - Chung,Tse-Yu, AU - Chen,Chii-Shiarng, AU - Jiang,Pei-Luen, AU - Tzen,Jason T C, Y1 - 2016/02/10/ PY - 2015/09/24/received PY - 2016/02/04/revised PY - 2016/02/06/accepted PY - 2016/2/22/entrez PY - 2016/2/22/pubmed PY - 2016/12/15/medline KW - Gymnosperm KW - Megagametophyte KW - Oil body KW - Pine KW - Steroleosin SP - 173 EP - 181 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 101 N2 - Three classes of integral proteins termed oleosin, caleosin and steroleosin have been identified in seed oil bodies of diverse angiosperm species. Recently, two oleosin isoforms and one caleosin were identified in megagametophyte oil bodies of pine (Pinus massoniana), a representative gymnosperm species. In this study, a putative steroleosin of approximately 41 kDa was observed in isolated oil bodies of pine megagametophytes, and its corresponding cDNA fragment was obtained by PCR cloning and further confirmed by mass spectrometric analysis. Phylogenetic tree analysis showed that pine steroleosin was evolutionarily more closely-related to steroleosin-B than steroleosin-A found in angiosperm seed oil bodies. As expected, artificial oil bodies constituted with recombinant steroleosin over-expressed in Escherichia coli were less stable and larger than native pine oil bodies. Filipin staining of artificial oil bodies sheltered by recombinant steroleosin with or without its sterol binding domain showed that the sterol binding domain was responsible for the sterol binding capability of steroleosin. Sterol-coupling dehydrogenase activity was demonstrated in artificial oil bodies constituted with recombinant steroleosin as well as in purified pine oil bodies. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/26897709/Identification_of_steroleosin_in_oil_bodies_of_pine_megagametophytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30033-X DB - PRIME DP - Unbound Medicine ER -