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Human cytomegalovirus UL49 encodes an early, virion-associated protein essential for virus growth in human foreskin fibroblasts.
Arch Virol. 2016 May; 161(5):1273-84.AV

Abstract

Despite recent results of deletion experiments showing that open reading frame (ORF) UL49 of human cytomegalovirus (HCMV) is essential, the expression, function and functional location of its encoded protein remain unknown. We generated an antibody specific for pUL49 to investigate the protein product encoded by the UL49 ORF and identified its function in HCMV-infected host foreskin fibroblasts. A bacterial artificial chromosome (BAC) of HCMV strain Towne (pRV-Towne) and the UL49-deleted mutant pRV-delUL49Towne were used to observe virus growth by plaque assay. Using a UL49-protein-binding antibody, we located pUL49 in the fibroblast cytoplasm. pUL49 exhibited expression kinetics resembling those of the class β-2 proteins and was detected in the virion tegument. Following deletion of UL49 ORF, the virus failed to replicate, but it could be recovered by addition of pUL49 from pCDNA3.1 (+)-UL49. Our findings indicate that UL49 ORF is essential for HCMV replication in host foreskin fibroblasts.

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Authors+Show Affiliations

Zhu F
Guangzhou Occupational Disease Preventive and Treatment Center, Guangzhou No. 12 Hospital Affiliated to Guangzhou Medical University, Guangzhou, 510620, China. chifengzhu@hotmail.com.
Yuan J
Department of Pathology, Guangdong Key Laboratory for Bioactive Drugs Research Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Li HJ
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Zeng ZF
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Luo ZW
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Li SQ
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
He CQ
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Jia XF
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Zhang X
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Zuo H
Guangzhou Occupational Disease Preventive and Treatment Center, Guangzhou No. 12 Hospital Affiliated to Guangzhou Medical University, Guangzhou, 510620, China.
Liu YM
Guangzhou Occupational Disease Preventive and Treatment Center, Guangzhou No. 12 Hospital Affiliated to Guangzhou Medical University, Guangzhou, 510620, China.
Chang M
Center for Musculoskeletal Research, University of Rochester, Rochester, NY, 14627, USA.
Li YQ
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Zhou TH
College of Life Science and Technology, Jinan University, Guangzhou, 510632, China. tzth@jnu.edu.cn.

MeSH

Base SequenceCell LineCytomegalovirusCytomegalovirus InfectionsFibroblastsForeskinGene Expression Regulation, ViralHumansMaleMicroscopy, FluorescenceMolecular Sequence DataViral ProteinsVirionVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26898402

Citation

Zhu, Feng, et al. "Human Cytomegalovirus UL49 Encodes an Early, Virion-associated Protein Essential for Virus Growth in Human Foreskin Fibroblasts." Archives of Virology, vol. 161, no. 5, 2016, pp. 1273-84.
Zhu F, Yuan J, Li HJ, et al. Human cytomegalovirus UL49 encodes an early, virion-associated protein essential for virus growth in human foreskin fibroblasts. Arch Virol. 2016;161(5):1273-84.
Zhu, F., Yuan, J., Li, H. J., Zeng, Z. F., Luo, Z. W., Li, S. Q., He, C. Q., Jia, X. F., Zhang, X., Zuo, H., Liu, Y. M., Chang, M., Li, Y. Q., & Zhou, T. H. (2016). Human cytomegalovirus UL49 encodes an early, virion-associated protein essential for virus growth in human foreskin fibroblasts. Archives of Virology, 161(5), 1273-84. https://doi.org/10.1007/s00705-016-2780-4
Zhu F, et al. Human Cytomegalovirus UL49 Encodes an Early, Virion-associated Protein Essential for Virus Growth in Human Foreskin Fibroblasts. Arch Virol. 2016;161(5):1273-84. PubMed PMID: 26898402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human cytomegalovirus UL49 encodes an early, virion-associated protein essential for virus growth in human foreskin fibroblasts. AU - Zhu,Feng, AU - Yuan,Jian, AU - Li,Hong-Jian, AU - Zeng,Zhi-Feng, AU - Luo,Zhi-Wen, AU - Li,Shi-Qian, AU - He,Chi-Qiang, AU - Jia,Xue-Fang, AU - Zhang,Xin, AU - Zuo,Hui, AU - Liu,Yi-Min, AU - Chang,Martin, AU - Li,Yue-Qin, AU - Zhou,Tian-Hong, Y1 - 2016/02/22/ PY - 2015/03/25/received PY - 2016/01/30/accepted PY - 2016/2/23/entrez PY - 2016/2/24/pubmed PY - 2016/8/31/medline SP - 1273 EP - 84 JF - Archives of virology JO - Arch Virol VL - 161 IS - 5 N2 - Despite recent results of deletion experiments showing that open reading frame (ORF) UL49 of human cytomegalovirus (HCMV) is essential, the expression, function and functional location of its encoded protein remain unknown. We generated an antibody specific for pUL49 to investigate the protein product encoded by the UL49 ORF and identified its function in HCMV-infected host foreskin fibroblasts. A bacterial artificial chromosome (BAC) of HCMV strain Towne (pRV-Towne) and the UL49-deleted mutant pRV-delUL49Towne were used to observe virus growth by plaque assay. Using a UL49-protein-binding antibody, we located pUL49 in the fibroblast cytoplasm. pUL49 exhibited expression kinetics resembling those of the class β-2 proteins and was detected in the virion tegument. Following deletion of UL49 ORF, the virus failed to replicate, but it could be recovered by addition of pUL49 from pCDNA3.1 (+)-UL49. Our findings indicate that UL49 ORF is essential for HCMV replication in host foreskin fibroblasts. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/26898402/Human_cytomegalovirus_UL49_encodes_an_early_virion_associated_protein_essential_for_virus_growth_in_human_foreskin_fibroblasts_ DB - PRIME DP - Unbound Medicine ER -