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Neuronal apoptosis may not contribute to the long-term cognitive dysfunction induced by a brief exposure to 2% sevoflurane in developing rats.
Biomed Pharmacother. 2016 Mar; 78:322-328.BP

Abstract

BACKGROUND

Sevoflurane is an inhaled anesthetic commonly used in the pediatric. Recent animal studies suggest that early exposure to high concentration of sevoflurane for a long duration can induce neuroapoptosis and later cognitive dysfunction. However, the neurodevelopmental impact induced by lower concentration and shorter exposure duration of sevoflurane is unclear. To investigate whether early exposure to 2% concentration of sevoflurane for a short duration (clinically relevant usage of sevoflurane) can also induce neuroapoptosis and later cognitive dysfunction.

METHODS

Rat pups were subjected to control group, 2% sevoflurane for 3h and 3% sevoflurane for 6h. TUNEL assay and apoptotic enzyme cleaved caspase-3 measured by western blot were used for detection of neuronal apoptosis in frontal cortex and CA1 region of hippocampus 24 after sevoflurane treatment. Long-term cognitive function was evaluated by Morris water maze and passive avoidance test as the rats grew up.

RESULTS

The apoptotic levels in frontal cortex and CA1 region were significantly increased after rats exposed to 3% sevoflurane for 6h (P<0.05), but not 2% sevoflurane for 3h (P>0.05). Exposure to both 2% sevoflurane for 3h and 3% sevoflurane for 6h could cause long-term cognitive dysfunction and animals exposed to 3% sevoflurane for 6h exhibited worse neurodevelopmental outcomes (P<0.05).

CONCLUSION

It was suggested that neuronal apoptosis might not contribute to long-term cognitive dysfunction induced by 2% concentration and short exposure time of sevoflurane. Our findings also suggested that the mechanisms of sevoflurane-induced neurodevelopmental impact might be various, depending on the concentration and exposure duration.

Authors+Show Affiliations

Department of Anesthesiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.Department of Anesthesiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.Department of Anesthesiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.Department of Anesthesiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.Department of Anesthesiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.Department of Anesthesiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China. Electronic address: dr_hongjiang@163.com.Department of Anesthesiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China. Electronic address: mzkyanj@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26898457

Citation

Lu, Yi, et al. "Neuronal Apoptosis May Not Contribute to the Long-term Cognitive Dysfunction Induced By a Brief Exposure to 2% Sevoflurane in Developing Rats." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 78, 2016, pp. 322-328.
Lu Y, Huang Y, Jiang J, et al. Neuronal apoptosis may not contribute to the long-term cognitive dysfunction induced by a brief exposure to 2% sevoflurane in developing rats. Biomed Pharmacother. 2016;78:322-328.
Lu, Y., Huang, Y., Jiang, J., Hu, R., Yang, Y., Jiang, H., & Yan, J. (2016). Neuronal apoptosis may not contribute to the long-term cognitive dysfunction induced by a brief exposure to 2% sevoflurane in developing rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 78, 322-328. https://doi.org/10.1016/j.biopha.2016.01.034
Lu Y, et al. Neuronal Apoptosis May Not Contribute to the Long-term Cognitive Dysfunction Induced By a Brief Exposure to 2% Sevoflurane in Developing Rats. Biomed Pharmacother. 2016;78:322-328. PubMed PMID: 26898457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal apoptosis may not contribute to the long-term cognitive dysfunction induced by a brief exposure to 2% sevoflurane in developing rats. AU - Lu,Yi, AU - Huang,Yan, AU - Jiang,Jue, AU - Hu,Rong, AU - Yang,Yaqiong, AU - Jiang,Hong, AU - Yan,Jia, Y1 - 2016/02/06/ PY - 2015/11/07/received PY - 2016/01/26/accepted PY - 2016/2/23/entrez PY - 2016/2/24/pubmed PY - 2016/12/15/medline KW - Anesthesia KW - Apoptosis KW - Developing brain KW - Neurodevelopment KW - Neurotoxicity KW - Sevoflurane SP - 322 EP - 328 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 78 N2 - BACKGROUND: Sevoflurane is an inhaled anesthetic commonly used in the pediatric. Recent animal studies suggest that early exposure to high concentration of sevoflurane for a long duration can induce neuroapoptosis and later cognitive dysfunction. However, the neurodevelopmental impact induced by lower concentration and shorter exposure duration of sevoflurane is unclear. To investigate whether early exposure to 2% concentration of sevoflurane for a short duration (clinically relevant usage of sevoflurane) can also induce neuroapoptosis and later cognitive dysfunction. METHODS: Rat pups were subjected to control group, 2% sevoflurane for 3h and 3% sevoflurane for 6h. TUNEL assay and apoptotic enzyme cleaved caspase-3 measured by western blot were used for detection of neuronal apoptosis in frontal cortex and CA1 region of hippocampus 24 after sevoflurane treatment. Long-term cognitive function was evaluated by Morris water maze and passive avoidance test as the rats grew up. RESULTS: The apoptotic levels in frontal cortex and CA1 region were significantly increased after rats exposed to 3% sevoflurane for 6h (P<0.05), but not 2% sevoflurane for 3h (P>0.05). Exposure to both 2% sevoflurane for 3h and 3% sevoflurane for 6h could cause long-term cognitive dysfunction and animals exposed to 3% sevoflurane for 6h exhibited worse neurodevelopmental outcomes (P<0.05). CONCLUSION: It was suggested that neuronal apoptosis might not contribute to long-term cognitive dysfunction induced by 2% concentration and short exposure time of sevoflurane. Our findings also suggested that the mechanisms of sevoflurane-induced neurodevelopmental impact might be various, depending on the concentration and exposure duration. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/26898457/Neuronal_apoptosis_may_not_contribute_to_the_long_term_cognitive_dysfunction_induced_by_a_brief_exposure_to_2_sevoflurane_in_developing_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(15)30366-8 DB - PRIME DP - Unbound Medicine ER -