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Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix tetrandus) Analyzed by UHPLC-Q/Orbitrap/MS/MS and Its Antioxidant Properties.
Molecules. 2016 Feb 23; 21(3):245.M

Abstract

Mass spectrometry has become a method of choice to characterize bioactive compounds in biological samples because of its sensitivity and selectivity. Hybrid ultra-HPLC hyphenated with Orbitrap mass analyzer is an innovative state of the art technology that allows fast and accurate metabolomic analyses. In this work the metabolites of a Chilean mistletoe endemic to the VIII region of Chile were investigated for the first time using UHPLC mass analysis (UHPLC-PDA-HESI-Orbitrap MS(n)). The anthocyanins, together with the non-pigmented phenolics were fingerprinted and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Six anthocyanins were identified, and among them, the 3-O-glycosides of delphinidin and cyanidin were the major ones. In addition, several phenolic acids (including feruloylquinic acid, feruloyl glucose, chlorogenic acid) and several flavonols (luteolin, quercetin, apigenin, isorhamnetin and glycoside derivatives) were also identified. The mistletoe leaves showed the highest antioxidant activity as measured by the DPPH radical bleaching, ferric reducing antioxidant power and superoxide anion scavenging activity tests (13.38 ± 0.47 µg/mL, 125.32 ± 5.96 µmolTE/g DW and 84.06 ± 4.59 at 100 µg/mL, respectively).

Authors+Show Affiliations

Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta 1240000, Chile. mario.simirgiotis@uantof.cl.Facultad de Ciencias de la Salud, Universidad Arturo Prat, Casilla 121, Iquique 1110939, Chile. elquispe@unap.cl.Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago 7800024, Chile. areche@uchile.cl.Departamento de Ciencias Químicas, Universidad Andres Bello, Campus Viña del Mar, Quillota 980, Viña del Mar 2520000, Chile. bsepulveda@uc.cl.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26907248

Citation

Simirgiotis, Mario J., et al. "Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix Tetrandus) Analyzed By UHPLC-Q/Orbitrap/MS/MS and Its Antioxidant Properties." Molecules (Basel, Switzerland), vol. 21, no. 3, 2016, p. 245.
Simirgiotis MJ, Quispe C, Areche C, et al. Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix tetrandus) Analyzed by UHPLC-Q/Orbitrap/MS/MS and Its Antioxidant Properties. Molecules. 2016;21(3):245.
Simirgiotis, M. J., Quispe, C., Areche, C., & Sepúlveda, B. (2016). Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix tetrandus) Analyzed by UHPLC-Q/Orbitrap/MS/MS and Its Antioxidant Properties. Molecules (Basel, Switzerland), 21(3), 245. https://doi.org/10.3390/molecules21030245
Simirgiotis MJ, et al. Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix Tetrandus) Analyzed By UHPLC-Q/Orbitrap/MS/MS and Its Antioxidant Properties. Molecules. 2016 Feb 23;21(3):245. PubMed PMID: 26907248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix tetrandus) Analyzed by UHPLC-Q/Orbitrap/MS/MS and Its Antioxidant Properties. AU - Simirgiotis,Mario J, AU - Quispe,Cristina, AU - Areche,Carlos, AU - Sepúlveda,Beatriz, Y1 - 2016/02/23/ PY - 2016/01/25/received PY - 2016/02/11/revised PY - 2016/02/18/accepted PY - 2016/2/25/entrez PY - 2016/2/26/pubmed PY - 2016/11/12/medline KW - anthocyanins KW - antioxidants KW - flavonoids KW - muérdago KW - phenolic acids KW - quintral del álamo KW - ultra HPLC-MS SP - 245 EP - 245 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 21 IS - 3 N2 - Mass spectrometry has become a method of choice to characterize bioactive compounds in biological samples because of its sensitivity and selectivity. Hybrid ultra-HPLC hyphenated with Orbitrap mass analyzer is an innovative state of the art technology that allows fast and accurate metabolomic analyses. In this work the metabolites of a Chilean mistletoe endemic to the VIII region of Chile were investigated for the first time using UHPLC mass analysis (UHPLC-PDA-HESI-Orbitrap MS(n)). The anthocyanins, together with the non-pigmented phenolics were fingerprinted and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Six anthocyanins were identified, and among them, the 3-O-glycosides of delphinidin and cyanidin were the major ones. In addition, several phenolic acids (including feruloylquinic acid, feruloyl glucose, chlorogenic acid) and several flavonols (luteolin, quercetin, apigenin, isorhamnetin and glycoside derivatives) were also identified. The mistletoe leaves showed the highest antioxidant activity as measured by the DPPH radical bleaching, ferric reducing antioxidant power and superoxide anion scavenging activity tests (13.38 ± 0.47 µg/mL, 125.32 ± 5.96 µmolTE/g DW and 84.06 ± 4.59 at 100 µg/mL, respectively). SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/26907248/Phenolic_Compounds_in_Chilean_Mistletoe__Quintral_Tristerix_tetrandus__Analyzed_by_UHPLC_Q/Orbitrap/MS/MS_and_Its_Antioxidant_Properties_ L2 - http://www.mdpi.com/resolver?pii=molecules21030245 DB - PRIME DP - Unbound Medicine ER -