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Isolation and molecular characterization of Leptospira borgpetersenii serovar Hardjo strain Hardjobovis in the urine of naturally infected cattle in Brazil.
Genet Mol Res 2016; 15(1)GM

Abstract

Most epidemiologic studies on bovine leptospirosis are based on serological tests that use antibodies against several serotypes, including the serovar Hardjo, which is widespread and considered to be the most adapted to bovine hosts. However, using only serological studies is not sufficient to identify and distinguish species of leptospires. The aim of this study was report the first isolation in Brazil of two strains serovar Hardjo obtained in urine samples from naturally infected cows in a small Brazilian dairy herd and find the genetic species and consequently the type strain Hardjobovis by molecular characterization. Fifteen dairy cows with a history of reproductive failure, such as abortion and infertility, were selected. Urine samples obtained from each animal were immediately seeded in tubes containing Ellinghausen-McCullough-Johnson-Harris culture medium. The identification of the isolates was performed by Multilocus variable-number tandem-repeat analysis (MLVA) technique and phylogenetic analysis of partial sequence of gene sec Y. From the 15 urine samples evaluated, two Leptospira were found and identified as the Londrina 49 and Londrina 54 strains. The MLVA profiles and sequencing of gene sec Y characterized the isolates as L. borgpetersenii serovar Hardjo strain Hadjobovis because it has different genetic pattern of Leptospira interrogans serovar Hardjo strain Hardjoprajitno. Therefore, more studies are needed including isolation and molecular characterization from regional strains to obtain a better knowledge about epidemiology of serovar Hardjo in bovine which may assist in future strategies of prevention and control of bovine leptospirosis.

Authors+Show Affiliations

Laboratório de Leptospirose, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brasil.Laboratório de Bacteriologia, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brasil.Laboratório de Medicina Veterinária Preventiva e Saúde Pública, Universidade Paranaense, Umuarama, PR, Brasil.Medicina Veterinária, Universidade Estadual de Maringá, Umuarama, PR, Brasil.Laboratório de Virologia Animal, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brasil.Laboratório de Virologia Animal, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brasil.Laboratório de Leptospirose, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brasil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26909976

Citation

Chideroli, R T., et al. "Isolation and Molecular Characterization of Leptospira Borgpetersenii Serovar Hardjo Strain Hardjobovis in the Urine of Naturally Infected Cattle in Brazil." Genetics and Molecular Research : GMR, vol. 15, no. 1, 2016.
Chideroli RT, Pereira UP, Gonçalves DD, et al. Isolation and molecular characterization of Leptospira borgpetersenii serovar Hardjo strain Hardjobovis in the urine of naturally infected cattle in Brazil. Genet Mol Res. 2016;15(1).
Chideroli, R. T., Pereira, U. P., Gonçalves, D. D., Nakamura, A. Y., Alfieri, A. A., Alfieri, A. F., & Freitas, J. C. (2016). Isolation and molecular characterization of Leptospira borgpetersenii serovar Hardjo strain Hardjobovis in the urine of naturally infected cattle in Brazil. Genetics and Molecular Research : GMR, 15(1), doi:10.4238/gmr.15018473.
Chideroli RT, et al. Isolation and Molecular Characterization of Leptospira Borgpetersenii Serovar Hardjo Strain Hardjobovis in the Urine of Naturally Infected Cattle in Brazil. Genet Mol Res. 2016 Feb 19;15(1) PubMed PMID: 26909976.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and molecular characterization of Leptospira borgpetersenii serovar Hardjo strain Hardjobovis in the urine of naturally infected cattle in Brazil. AU - Chideroli,R T, AU - Pereira,U P, AU - Gonçalves,D D, AU - Nakamura,A Y, AU - Alfieri,A A, AU - Alfieri,A F, AU - Freitas,J C, Y1 - 2016/02/19/ PY - 2016/2/25/entrez PY - 2016/2/26/pubmed PY - 2016/11/12/medline JF - Genetics and molecular research : GMR JO - Genet. Mol. Res. VL - 15 IS - 1 N2 - Most epidemiologic studies on bovine leptospirosis are based on serological tests that use antibodies against several serotypes, including the serovar Hardjo, which is widespread and considered to be the most adapted to bovine hosts. However, using only serological studies is not sufficient to identify and distinguish species of leptospires. The aim of this study was report the first isolation in Brazil of two strains serovar Hardjo obtained in urine samples from naturally infected cows in a small Brazilian dairy herd and find the genetic species and consequently the type strain Hardjobovis by molecular characterization. Fifteen dairy cows with a history of reproductive failure, such as abortion and infertility, were selected. Urine samples obtained from each animal were immediately seeded in tubes containing Ellinghausen-McCullough-Johnson-Harris culture medium. The identification of the isolates was performed by Multilocus variable-number tandem-repeat analysis (MLVA) technique and phylogenetic analysis of partial sequence of gene sec Y. From the 15 urine samples evaluated, two Leptospira were found and identified as the Londrina 49 and Londrina 54 strains. The MLVA profiles and sequencing of gene sec Y characterized the isolates as L. borgpetersenii serovar Hardjo strain Hadjobovis because it has different genetic pattern of Leptospira interrogans serovar Hardjo strain Hardjoprajitno. Therefore, more studies are needed including isolation and molecular characterization from regional strains to obtain a better knowledge about epidemiology of serovar Hardjo in bovine which may assist in future strategies of prevention and control of bovine leptospirosis. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/26909976/Isolation_and_molecular_characterization_of_Leptospira_borgpetersenii_serovar_Hardjo_strain_Hardjobovis_in_the_urine_of_naturally_infected_cattle_in_Brazil_ L2 - http://www.geneticsmr.com/articles/6041 DB - PRIME DP - Unbound Medicine ER -