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Effects of acetate on Kluyveromyces marxianus DSM 5422 growth and metabolism.
Appl Microbiol Biotechnol. 2016 May; 100(10):4585-94.AM

Abstract

Metabolically active cells produce a wide array of metabolites that can inhibit their growth. Acetate is a widely known preservative, and it is also produced by yeast cells during their growth. Kluyveromyces marxianus DSM 5422 is a promising yeast strain that could be employed in biotechnological processes, but the knowledge of its stress physiology is scarce. Here, we investigate the effects of acetate on growth and changes in cell population structure during adaptation to elevated concentrations of acetate in K. marxianus DSM 5422. Our results indicate that acetate inhibits growth in a pH-dependent manner and has pronounced effects if yeast is grown on lactose or galactose. When challenged with acetate, culture extends lag phase, during which cells adapt to elevated acetate concentrations, and growth reoccurs, albeit at a slower rate, when majority of the population is acetate resistant. Acetate resistance is maintained only if acetate is present in the media or if the culture has reached end of active growth phase. This study shows possible caveats in lactose fermentation with K. marxianus and gives a further perspective in non-conventional yeast applications in biotechnology.

Authors+Show Affiliations

Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str 1, Riga, LV1004, Latvia.Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str 1, Riga, LV1004, Latvia. agnese.kokina@lu.lv.Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str 1, Riga, LV1004, Latvia.Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str 1, Riga, LV1004, Latvia.Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str 1, Riga, LV1004, Latvia.Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str 1, Riga, LV1004, Latvia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26910042

Citation

Martynova, Jekaterina, et al. "Effects of Acetate On Kluyveromyces Marxianus DSM 5422 Growth and Metabolism." Applied Microbiology and Biotechnology, vol. 100, no. 10, 2016, pp. 4585-94.
Martynova J, Kokina A, Kibilds J, et al. Effects of acetate on Kluyveromyces marxianus DSM 5422 growth and metabolism. Appl Microbiol Biotechnol. 2016;100(10):4585-94.
Martynova, J., Kokina, A., Kibilds, J., Liepins, J., Scerbaka, R., & Vigants, A. (2016). Effects of acetate on Kluyveromyces marxianus DSM 5422 growth and metabolism. Applied Microbiology and Biotechnology, 100(10), 4585-94. https://doi.org/10.1007/s00253-016-7392-0
Martynova J, et al. Effects of Acetate On Kluyveromyces Marxianus DSM 5422 Growth and Metabolism. Appl Microbiol Biotechnol. 2016;100(10):4585-94. PubMed PMID: 26910042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of acetate on Kluyveromyces marxianus DSM 5422 growth and metabolism. AU - Martynova,Jekaterina, AU - Kokina,Agnese, AU - Kibilds,Juris, AU - Liepins,Janis, AU - Scerbaka,Rita, AU - Vigants,Armands, Y1 - 2016/02/24/ PY - 2015/09/03/received PY - 2016/02/11/accepted PY - 2016/02/09/revised PY - 2016/2/25/entrez PY - 2016/2/26/pubmed PY - 2017/3/10/medline KW - Acetate stress KW - Kluyveromyces marxianus KW - Lactose KW - Non-conventional yeasts KW - Population heterogenity SP - 4585 EP - 94 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 100 IS - 10 N2 - Metabolically active cells produce a wide array of metabolites that can inhibit their growth. Acetate is a widely known preservative, and it is also produced by yeast cells during their growth. Kluyveromyces marxianus DSM 5422 is a promising yeast strain that could be employed in biotechnological processes, but the knowledge of its stress physiology is scarce. Here, we investigate the effects of acetate on growth and changes in cell population structure during adaptation to elevated concentrations of acetate in K. marxianus DSM 5422. Our results indicate that acetate inhibits growth in a pH-dependent manner and has pronounced effects if yeast is grown on lactose or galactose. When challenged with acetate, culture extends lag phase, during which cells adapt to elevated acetate concentrations, and growth reoccurs, albeit at a slower rate, when majority of the population is acetate resistant. Acetate resistance is maintained only if acetate is present in the media or if the culture has reached end of active growth phase. This study shows possible caveats in lactose fermentation with K. marxianus and gives a further perspective in non-conventional yeast applications in biotechnology. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/26910042/Effects_of_acetate_on_Kluyveromyces_marxianus_DSM_5422_growth_and_metabolism_ L2 - https://dx.doi.org/10.1007/s00253-016-7392-0 DB - PRIME DP - Unbound Medicine ER -