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Evaluation of Hepatoprotective activity of Eriocaulon quinquangulare in vitro using porcine liver slices against ethanol induced liver toxicity and free radical scavenging capacity.

Abstract

BACKGROUND

Production of reactive oxygen species is a common cause in alcohol induced liver diseases. Decoction prepared from the whole plant of Eriocaulon quinquingulare is prescribed to treat liver disorders. The aim of this study was to investigate the hepatoprotective activity and antioxidant capacity of the water extract of E. quinquangulare in vitro.

METHOD

The aqueous extract of the whole plant of E. quinquangulare (AEQ) was investigated for its phytochemical constituents, antioxidant and membrane stabilization properties in-vitro. The antioxidant activities of AEQ were investigated using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical, nitric oxide scavenging and ferric reducing antioxidant power (FRAP) assays. Membrane stabilizing effect of the extract was determined by hypotonic solution induced human erythrocyte hemolytic assay (HEHA). Further, hepatoprotective activity against ethanol induced hepatotoxicity was carried out using porcine liver slices.

RESULTS

The total phenolics and flavonoids were 10.3 ± 1.6 w/w % gallic acid equivalents and 45.6 ± 3.8 w/w % (-)-epigallocatechin gallate equivalents respectively. The values of EC50 for DPPH, hydroxyl radical and nitric oxide scavenging assays were 37.2 ± 1.7 μg/ml, 170.5 ± 6.6 μg/ml and 31.8 ± 2.2 μg/ml respectively. The reducing capability of AEQ was 6.9 ± 0.2 w/w % L-ascorbic acid equivalents in the FRAP assay. For hypotonic solution induced HEHA, the IC50 was 1.79 ± 0.04 mg/ml. A significant decrease (p < 0.05) was observed in ALT, AST and LDH release from the liver slices treated with AEQ compared to the ethanol treated liver slices. A significant reduction in lipid peroxidation (p < 0.05) was also observed in liver slices treated with the plant extract compared to that of the ethanol treated liver slices.

CONCLUSIONS

The results suggest AEQ possess hepatoprotective activity against ethanol induced liver toxicity of porcine liver slices which can be attributed to antioxidant properties and membrane stabilizing effects caused by the plant material.

Authors+Show Affiliations

Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo, 08, Sri Lanka. dilankafdo86@gmail.com. College of Chemical Sciences, Institute of Chemistry Ceylon, 341/22, Kotte Road, Welikada, Rajagiriya, Sri Lanka. dilankafdo86@gmail.com.Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo, 08, Sri Lanka. indunilsree@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26912176

Citation

Fernando, Chamira Dilanka, and Preethi Soysa. "Evaluation of Hepatoprotective Activity of Eriocaulon Quinquangulare in Vitro Using Porcine Liver Slices Against Ethanol Induced Liver Toxicity and Free Radical Scavenging Capacity." BMC Complementary and Alternative Medicine, vol. 16, 2016, p. 74.
Fernando CD, Soysa P. Evaluation of Hepatoprotective activity of Eriocaulon quinquangulare in vitro using porcine liver slices against ethanol induced liver toxicity and free radical scavenging capacity. BMC Complement Altern Med. 2016;16:74.
Fernando, C. D., & Soysa, P. (2016). Evaluation of Hepatoprotective activity of Eriocaulon quinquangulare in vitro using porcine liver slices against ethanol induced liver toxicity and free radical scavenging capacity. BMC Complementary and Alternative Medicine, 16, p. 74. doi:10.1186/s12906-016-1044-x.
Fernando CD, Soysa P. Evaluation of Hepatoprotective Activity of Eriocaulon Quinquangulare in Vitro Using Porcine Liver Slices Against Ethanol Induced Liver Toxicity and Free Radical Scavenging Capacity. BMC Complement Altern Med. 2016 Feb 24;16:74. PubMed PMID: 26912176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Hepatoprotective activity of Eriocaulon quinquangulare in vitro using porcine liver slices against ethanol induced liver toxicity and free radical scavenging capacity. AU - Fernando,Chamira Dilanka, AU - Soysa,Preethi, Y1 - 2016/02/24/ PY - 2015/09/24/received PY - 2016/02/12/accepted PY - 2016/2/26/entrez PY - 2016/2/26/pubmed PY - 2016/9/20/medline SP - 74 EP - 74 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 16 N2 - BACKGROUND: Production of reactive oxygen species is a common cause in alcohol induced liver diseases. Decoction prepared from the whole plant of Eriocaulon quinquingulare is prescribed to treat liver disorders. The aim of this study was to investigate the hepatoprotective activity and antioxidant capacity of the water extract of E. quinquangulare in vitro. METHOD: The aqueous extract of the whole plant of E. quinquangulare (AEQ) was investigated for its phytochemical constituents, antioxidant and membrane stabilization properties in-vitro. The antioxidant activities of AEQ were investigated using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical, nitric oxide scavenging and ferric reducing antioxidant power (FRAP) assays. Membrane stabilizing effect of the extract was determined by hypotonic solution induced human erythrocyte hemolytic assay (HEHA). Further, hepatoprotective activity against ethanol induced hepatotoxicity was carried out using porcine liver slices. RESULTS: The total phenolics and flavonoids were 10.3 ± 1.6 w/w % gallic acid equivalents and 45.6 ± 3.8 w/w % (-)-epigallocatechin gallate equivalents respectively. The values of EC50 for DPPH, hydroxyl radical and nitric oxide scavenging assays were 37.2 ± 1.7 μg/ml, 170.5 ± 6.6 μg/ml and 31.8 ± 2.2 μg/ml respectively. The reducing capability of AEQ was 6.9 ± 0.2 w/w % L-ascorbic acid equivalents in the FRAP assay. For hypotonic solution induced HEHA, the IC50 was 1.79 ± 0.04 mg/ml. A significant decrease (p < 0.05) was observed in ALT, AST and LDH release from the liver slices treated with AEQ compared to the ethanol treated liver slices. A significant reduction in lipid peroxidation (p < 0.05) was also observed in liver slices treated with the plant extract compared to that of the ethanol treated liver slices. CONCLUSIONS: The results suggest AEQ possess hepatoprotective activity against ethanol induced liver toxicity of porcine liver slices which can be attributed to antioxidant properties and membrane stabilizing effects caused by the plant material. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/26912176/Evaluation_of_Hepatoprotective_activity_of_Eriocaulon_quinquangulare_in_vitro_using_porcine_liver_slices_against_ethanol_induced_liver_toxicity_and_free_radical_scavenging_capacity_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1044-x DB - PRIME DP - Unbound Medicine ER -