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Evaluation of cephamycins as supplements to selective agar for detecting Campylobacter spp. in chicken carcass rinses.
Int J Food Microbiol. 2016 Apr 16; 223:75-8.IJ

Abstract

Although cefoperazone is the most commonly used antibiotic in Campylobacter-selective media, the distribution of cefoperazone-resistant bacteria such as extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is increasing. Here we evaluated the potential of cephamycins for use as supplements to improve modified charcoal-cefoperazone-deoxycholate agar (mCCDA) by replacing cefoperazone with the same concentrations (32 mg/L) of cefotetan (modified charcoal-cefotetan-deoxycholate agar, mCCtDA) and cefoxitin (modified charcoal-cefoxitin-deoxycholate agar, mCCxDA). In chicken carcass rinse samples, the number of mCCDA plates detecting for Campylobacter (18/70, 26%) was significantly lower than that of mCCtDA (42/70, 60%) or mCCxDA plates (40/70, 57%). The number of mCCDA plates (70/70, 100%) that were contaminated with non-Campylobacter species was significantly higher than that of mCCtDA (20/70, 29%) or mCCxDA plates (21/70, 30%). The most common competing species identified using mCCDA was ESBL-producing E. coli, while Pseudomonas species frequently appeared on mCCtDA and mCCxDA.

Authors+Show Affiliations

Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.Department of Food & Nutrition College of Human Ecology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea.Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea. Electronic address: bracstu3@konkuk.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26915052

Citation

Chon, Jung-Whan, et al. "Evaluation of Cephamycins as Supplements to Selective Agar for Detecting Campylobacter Spp. in Chicken Carcass Rinses." International Journal of Food Microbiology, vol. 223, 2016, pp. 75-8.
Chon JW, Kim YJ, Kim HS, et al. Evaluation of cephamycins as supplements to selective agar for detecting Campylobacter spp. in chicken carcass rinses. Int J Food Microbiol. 2016;223:75-8.
Chon, J. W., Kim, Y. J., Kim, H. S., Kim, D. H., Kim, H., Song, K. Y., Sung, K., & Seo, K. H. (2016). Evaluation of cephamycins as supplements to selective agar for detecting Campylobacter spp. in chicken carcass rinses. International Journal of Food Microbiology, 223, 75-8. https://doi.org/10.1016/j.ijfoodmicro.2016.01.019
Chon JW, et al. Evaluation of Cephamycins as Supplements to Selective Agar for Detecting Campylobacter Spp. in Chicken Carcass Rinses. Int J Food Microbiol. 2016 Apr 16;223:75-8. PubMed PMID: 26915052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of cephamycins as supplements to selective agar for detecting Campylobacter spp. in chicken carcass rinses. AU - Chon,Jung-Whan, AU - Kim,Young-Ji, AU - Kim,Hong-Seok, AU - Kim,Dong-Hyeon, AU - Kim,Hyunsook, AU - Song,Kwang-Young, AU - Sung,Kidon, AU - Seo,Kun-Ho, Y1 - 2016/01/28/ PY - 2015/04/20/received PY - 2015/11/30/revised PY - 2016/01/25/accepted PY - 2016/2/26/entrez PY - 2016/2/26/pubmed PY - 2016/8/20/medline KW - Campylobacter KW - Cefotetan KW - Cefoxitin KW - Chicken KW - ESBL SP - 75 EP - 8 JF - International journal of food microbiology JO - Int. J. Food Microbiol. VL - 223 N2 - Although cefoperazone is the most commonly used antibiotic in Campylobacter-selective media, the distribution of cefoperazone-resistant bacteria such as extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is increasing. Here we evaluated the potential of cephamycins for use as supplements to improve modified charcoal-cefoperazone-deoxycholate agar (mCCDA) by replacing cefoperazone with the same concentrations (32 mg/L) of cefotetan (modified charcoal-cefotetan-deoxycholate agar, mCCtDA) and cefoxitin (modified charcoal-cefoxitin-deoxycholate agar, mCCxDA). In chicken carcass rinse samples, the number of mCCDA plates detecting for Campylobacter (18/70, 26%) was significantly lower than that of mCCtDA (42/70, 60%) or mCCxDA plates (40/70, 57%). The number of mCCDA plates (70/70, 100%) that were contaminated with non-Campylobacter species was significantly higher than that of mCCtDA (20/70, 29%) or mCCxDA plates (21/70, 30%). The most common competing species identified using mCCDA was ESBL-producing E. coli, while Pseudomonas species frequently appeared on mCCtDA and mCCxDA. SN - 1879-3460 UR - https://www.unboundmedicine.com/medline/citation/26915052/Evaluation_of_cephamycins_as_supplements_to_selective_agar_for_detecting_Campylobacter_spp._in_chicken_carcass_rinses L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(16)30020-4 DB - PRIME DP - Unbound Medicine ER -