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Fabrication, characterization and biomedical application of two-nozzle electrospun polycaprolactone/zein-calcium lactate composite nonwoven mat.
J Mech Behav Biomed Mater. 2016 07; 60:312-323.JM

Abstract

The objective of the current work is to incorporate calcium lactate (CL) into polycaprolactone (PCL)/zein composite micro/nanofibrous scaffolds via electrospinning to engineer bone tissue. In this study, a composite micro/nano fibrous scaffold was fabricated using a single two-nozzle electrospinning system to combine indicative nanofibers from a blended solution of zein-CL and micro-sized fibers from a PCL solution. Incorporation of the CL into the PCL/zein fibers were shown to improve the wettability, tensile strength and biological activity of the composite mats. Moreover, the composite mats have a high efficiency to nucleate calcium phosphate from simulated body fluid (SBF) solution. An in vitro cell culture with osteoblast cells demonstrated that the electrospun composite mats possessed improved biological properties, including a better cell adhesion, spread and proliferation. This study has demonstrated that the PCL/zein-CL composite provides a simple platform to fabricate a new biomimetic scaffold for bone tissue engineering, which can recapitulate both the morphology of extracellular matrix and composition of the bone.

Authors+Show Affiliations

Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Center for Translational Medicine Research and Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, China.Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: biochan@jbnu.ac.kr.Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: chskim@jbnu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26919567

Citation

Liao, Nina, et al. "Fabrication, Characterization and Biomedical Application of Two-nozzle Electrospun Polycaprolactone/zein-calcium Lactate Composite Nonwoven Mat." Journal of the Mechanical Behavior of Biomedical Materials, vol. 60, 2016, pp. 312-323.
Liao N, Joshi MK, Tiwari AP, et al. Fabrication, characterization and biomedical application of two-nozzle electrospun polycaprolactone/zein-calcium lactate composite nonwoven mat. J Mech Behav Biomed Mater. 2016;60:312-323.
Liao, N., Joshi, M. K., Tiwari, A. P., Park, C. H., & Kim, C. S. (2016). Fabrication, characterization and biomedical application of two-nozzle electrospun polycaprolactone/zein-calcium lactate composite nonwoven mat. Journal of the Mechanical Behavior of Biomedical Materials, 60, 312-323. https://doi.org/10.1016/j.jmbbm.2016.02.006
Liao N, et al. Fabrication, Characterization and Biomedical Application of Two-nozzle Electrospun Polycaprolactone/zein-calcium Lactate Composite Nonwoven Mat. J Mech Behav Biomed Mater. 2016;60:312-323. PubMed PMID: 26919567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fabrication, characterization and biomedical application of two-nozzle electrospun polycaprolactone/zein-calcium lactate composite nonwoven mat. AU - Liao,Nina, AU - Joshi,Mahesh Kumar, AU - Tiwari,Arjun Prasad, AU - Park,Chan-Hee, AU - Kim,Cheol Sang, Y1 - 2016/02/09/ PY - 2015/11/11/received PY - 2016/01/20/revised PY - 2016/02/02/accepted PY - 2016/2/27/entrez PY - 2016/2/27/pubmed PY - 2017/12/2/medline KW - Biomedical application KW - Bone tissue KW - Calcium lactate KW - Micro-nano fibers KW - Two-nozzle electrospinning SP - 312 EP - 323 JF - Journal of the mechanical behavior of biomedical materials JO - J Mech Behav Biomed Mater VL - 60 N2 - The objective of the current work is to incorporate calcium lactate (CL) into polycaprolactone (PCL)/zein composite micro/nanofibrous scaffolds via electrospinning to engineer bone tissue. In this study, a composite micro/nano fibrous scaffold was fabricated using a single two-nozzle electrospinning system to combine indicative nanofibers from a blended solution of zein-CL and micro-sized fibers from a PCL solution. Incorporation of the CL into the PCL/zein fibers were shown to improve the wettability, tensile strength and biological activity of the composite mats. Moreover, the composite mats have a high efficiency to nucleate calcium phosphate from simulated body fluid (SBF) solution. An in vitro cell culture with osteoblast cells demonstrated that the electrospun composite mats possessed improved biological properties, including a better cell adhesion, spread and proliferation. This study has demonstrated that the PCL/zein-CL composite provides a simple platform to fabricate a new biomimetic scaffold for bone tissue engineering, which can recapitulate both the morphology of extracellular matrix and composition of the bone. SN - 1878-0180 UR - https://www.unboundmedicine.com/medline/citation/26919567/Fabrication_characterization_and_biomedical_application_of_two_nozzle_electrospun_polycaprolactone/zein_calcium_lactate_composite_nonwoven_mat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1751-6161(16)00044-8 DB - PRIME DP - Unbound Medicine ER -