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Nitrogen-doped graphene-chitosan matrix based efficient chemiluminescent immunosensor for detection of chicken interleukin-4.
Biosens Bioelectron. 2017 Mar 15; 89(Pt 1):558-564.BB

Abstract

Chicken interleukin-4 (ChIL-4), which is released by activated type 2 helper (Th2) cells following their stimulation in vitro, is an important indicator for the study of cell-mediated immunity in chickens after infection or vaccination. In this work, the first ChIL-4 chemiluminescent (CL) immunosensor was developed via the immobilization of monoclonal ChIL-4 antibodies on a nitrogen-doped graphene (NG)-chitosan nanocomposite matrix. NG nanosheets were used for the first time in the CL immunoassay to provide a biocompatible microenvironment for the immobilized capture antibody. The ChIL-4 immunosensor was characterized systematically. The proposed immunosensor displayed a wide linear range from 0.05 to 70ngmL-1 and a low detection limit of 0.02ngmL-1 at a signal-to-noise ratio of 3. Compared to traditional assay methods, this system was more flexible, simple, rapid, and sensitive. Moreover, this CL immunoassay system had an excellent detection and fabrication reproducibility, a high specificity, an acceptable accuracy, and a high stability. This work enables the specific detection of ChIL-4 and the further study of its role in the immune responses of poultry.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China. Electronic address: zjyang@yzu.edu.cn.School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.School of Medicine; Jiangsu Key Lab of Zoonosis, Yangzhou University, Yangzhou 225002, PR China. Electronic address: daihua@yzu.edu.cn.School of Medicine; Jiangsu Key Lab of Zoonosis, Yangzhou University, Yangzhou 225002, PR China.School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

26920112

Citation

Yang, Zhanjun, et al. "Nitrogen-doped Graphene-chitosan Matrix Based Efficient Chemiluminescent Immunosensor for Detection of Chicken Interleukin-4." Biosensors & Bioelectronics, vol. 89, no. Pt 1, 2017, pp. 558-564.
Yang Z, Lu M, Li J, et al. Nitrogen-doped graphene-chitosan matrix based efficient chemiluminescent immunosensor for detection of chicken interleukin-4. Biosens Bioelectron. 2017;89(Pt 1):558-564.
Yang, Z., Lu, M., Li, J., Tan, Z., Dai, H., Jiao, X., & Hu, X. (2017). Nitrogen-doped graphene-chitosan matrix based efficient chemiluminescent immunosensor for detection of chicken interleukin-4. Biosensors & Bioelectronics, 89(Pt 1), 558-564. https://doi.org/10.1016/j.bios.2016.02.046
Yang Z, et al. Nitrogen-doped Graphene-chitosan Matrix Based Efficient Chemiluminescent Immunosensor for Detection of Chicken Interleukin-4. Biosens Bioelectron. 2017 Mar 15;89(Pt 1):558-564. PubMed PMID: 26920112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitrogen-doped graphene-chitosan matrix based efficient chemiluminescent immunosensor for detection of chicken interleukin-4. AU - Yang,Zhanjun, AU - Lu,Mimi, AU - Li,Juan, AU - Tan,Zining, AU - Dai,Hua, AU - Jiao,Xin'an, AU - Hu,Xiaoya, Y1 - 2016/02/17/ PY - 2016/02/15/received PY - 2016/02/16/accepted PY - 2016/2/28/pubmed PY - 2017/2/25/medline PY - 2016/2/28/entrez KW - Chemiluminescent KW - Chicken interleukin-4 KW - Cytokine KW - Immunoassay KW - Nitrogen-doped graphene SP - 558 EP - 564 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 89 IS - Pt 1 N2 - Chicken interleukin-4 (ChIL-4), which is released by activated type 2 helper (Th2) cells following their stimulation in vitro, is an important indicator for the study of cell-mediated immunity in chickens after infection or vaccination. In this work, the first ChIL-4 chemiluminescent (CL) immunosensor was developed via the immobilization of monoclonal ChIL-4 antibodies on a nitrogen-doped graphene (NG)-chitosan nanocomposite matrix. NG nanosheets were used for the first time in the CL immunoassay to provide a biocompatible microenvironment for the immobilized capture antibody. The ChIL-4 immunosensor was characterized systematically. The proposed immunosensor displayed a wide linear range from 0.05 to 70ngmL-1 and a low detection limit of 0.02ngmL-1 at a signal-to-noise ratio of 3. Compared to traditional assay methods, this system was more flexible, simple, rapid, and sensitive. Moreover, this CL immunoassay system had an excellent detection and fabrication reproducibility, a high specificity, an acceptable accuracy, and a high stability. This work enables the specific detection of ChIL-4 and the further study of its role in the immune responses of poultry. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/26920112/Nitrogen_doped_graphene_chitosan_matrix_based_efficient_chemiluminescent_immunosensor_for_detection_of_chicken_interleukin_4_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(16)30153-1 DB - PRIME DP - Unbound Medicine ER -