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Fate of silver nanoparticles in wastewater and immunotoxic effects on rainbow trout.
Aquat Toxicol. 2016 May; 174:70-81.AT

Abstract

Silver nanoparticles (AgNPs) are currently used in technology, medicine and consumer products, even though the fate and the ecotoxicological risks on aquatic organisms of these new materials are not well known. The purpose of this study was to investigate the fate, bioavailability of AgNPs and their effects on fish in presence of municipal effluents. Juvenile rainbow trout were exposed for 96h to 40μg/L of AgNPs or 4μg/L of dissolved silver (AgNO3) in diluted (10%) municipal wastewater. Silver (Ag) concentrations were measured both on water samples and fish tissues (liver and gills). Toxicity was investigated by following immunological parameters in the pronephros (viability, phagocytosis) and biomarkers in liver and gills (cyclooxygenase activity, lipid peroxidation, glutathione-S-transferase, metallothioneins, DNA strand breaks and labile zinc). Results indicated that AgNPs appeared as small non-charged aggregates in wastewaters (11.7±1.4nm). In gills, the exposure to AgNPs induced morphological modifications without visible nanoparticle bioaccumulation. Dissolved Ag(+) was bioavailable in diluted effluent and induced oxidative stress (lipid peroxidation), labile zinc and a marginal decrease in superoxide dismutase in fish gills. Ag(+) also increased significantly metallothionein levels and inhibited the DNA repair activity in the liver. Finally, the two silver forms were found in liver and induced immunosuppression and inflammation (increase in cyclooxygenase activity). This study demonstrated that both forms of Ag produced harmful effects and AgNPs in wastewater were bioavailable to fish despite of their formation of aggregates.

Authors+Show Affiliations

Aquatic Contaminants Research Division, Environment Canada, 105 McGill Street, Montreal, Quebec H2Y 2E7, Canada. Electronic address: audrey.bruneau@gmail.com.Aquatic Contaminants Research Division, Environment Canada, 105 McGill Street, Montreal, Quebec H2Y 2E7, Canada.Aquatic Contaminants Research Division, Environment Canada, 105 McGill Street, Montreal, Quebec H2Y 2E7, Canada.Aquatic Contaminants Research Division, Environment Canada, 105 McGill Street, Montreal, Quebec H2Y 2E7, Canada.Aquatic Contaminants Research Division, Environment Canada, 105 McGill Street, Montreal, Quebec H2Y 2E7, Canada. Electronic address: christian.gagnon@ec.gc.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26921728

Citation

Bruneau, A, et al. "Fate of Silver Nanoparticles in Wastewater and Immunotoxic Effects On Rainbow Trout." Aquatic Toxicology (Amsterdam, Netherlands), vol. 174, 2016, pp. 70-81.
Bruneau A, Turcotte P, Pilote M, et al. Fate of silver nanoparticles in wastewater and immunotoxic effects on rainbow trout. Aquat Toxicol. 2016;174:70-81.
Bruneau, A., Turcotte, P., Pilote, M., Gagné, F., & Gagnon, C. (2016). Fate of silver nanoparticles in wastewater and immunotoxic effects on rainbow trout. Aquatic Toxicology (Amsterdam, Netherlands), 174, 70-81. https://doi.org/10.1016/j.aquatox.2016.02.013
Bruneau A, et al. Fate of Silver Nanoparticles in Wastewater and Immunotoxic Effects On Rainbow Trout. Aquat Toxicol. 2016;174:70-81. PubMed PMID: 26921728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fate of silver nanoparticles in wastewater and immunotoxic effects on rainbow trout. AU - Bruneau,A, AU - Turcotte,P, AU - Pilote,M, AU - Gagné,F, AU - Gagnon,C, Y1 - 2016/02/22/ PY - 2015/06/26/received PY - 2016/02/18/revised PY - 2016/02/18/accepted PY - 2016/2/28/entrez PY - 2016/2/28/pubmed PY - 2016/9/9/medline KW - Biomarkers KW - Nanoparticle fate KW - Nanotoxicity KW - Rainbow trout KW - Silver ion KW - Wastewater SP - 70 EP - 81 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 174 N2 - Silver nanoparticles (AgNPs) are currently used in technology, medicine and consumer products, even though the fate and the ecotoxicological risks on aquatic organisms of these new materials are not well known. The purpose of this study was to investigate the fate, bioavailability of AgNPs and their effects on fish in presence of municipal effluents. Juvenile rainbow trout were exposed for 96h to 40μg/L of AgNPs or 4μg/L of dissolved silver (AgNO3) in diluted (10%) municipal wastewater. Silver (Ag) concentrations were measured both on water samples and fish tissues (liver and gills). Toxicity was investigated by following immunological parameters in the pronephros (viability, phagocytosis) and biomarkers in liver and gills (cyclooxygenase activity, lipid peroxidation, glutathione-S-transferase, metallothioneins, DNA strand breaks and labile zinc). Results indicated that AgNPs appeared as small non-charged aggregates in wastewaters (11.7±1.4nm). In gills, the exposure to AgNPs induced morphological modifications without visible nanoparticle bioaccumulation. Dissolved Ag(+) was bioavailable in diluted effluent and induced oxidative stress (lipid peroxidation), labile zinc and a marginal decrease in superoxide dismutase in fish gills. Ag(+) also increased significantly metallothionein levels and inhibited the DNA repair activity in the liver. Finally, the two silver forms were found in liver and induced immunosuppression and inflammation (increase in cyclooxygenase activity). This study demonstrated that both forms of Ag produced harmful effects and AgNPs in wastewater were bioavailable to fish despite of their formation of aggregates. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/26921728/Fate_of_silver_nanoparticles_in_wastewater_and_immunotoxic_effects_on_rainbow_trout_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(16)30043-1 DB - PRIME DP - Unbound Medicine ER -