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McGISH identification and phenotypic description of leaf rust and yellow rust resistant partial amphiploids originating from a wheat × Thinopyrum synthetic hybrid cross.
J Appl Genet. 2016 Nov; 57(4):427-437.JA

Abstract

A Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid wheatgrass is an excellent source of leaf and stem rust resistance produced by N.V.Tsitsin. Wheat line Mv9kr1 was crossed with this hybrid (Agropyron glael) in Hungary in order to transfer its advantageous agronomic traits into wheat. As the wheat parent was susceptible to leaf rust, the transfer of resistance was easily recognizable in the progenies. Three different partial amphiploid lines with leaf rust resistance were selected from the wheat/Thinopyrum hybrid derivatives by multicolour genomic in situ hybridization. Chromosome counting on the partial amphiploids revealed 58 chromosomes (18 wheatgrass) in line 194, 56 (14 wheatgrass) in line 195 and 54 (12 wheatgrass) in line 196. The wheat chromosomes present in these lines were identified and the wheatgrass chromosomes were characterized by fluorescence in situ hybridization using the repetitive DNA probes Afa-family, pSc119.2 and pTa71. The 3D wheat chromosome was missing from the lines. Molecular marker analysis showed the presence of the Lr24 leaf rust resistance gene in lines 195 and 196. The morphological traits were evaluated in the field during two consecutive seasons in two different locations.

Links

Authors+Show Affiliations

Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.

Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.

Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.

Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.

Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.

Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.

Molnár-Láng M

Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary. molnar.marta@agrar.mta.hu.

MeSH

BasidiomycotaChromosomes, PlantDisease ResistanceGenetic MarkersHybridization, GeneticIn Situ Hybridization, FluorescencePhenotypePlant DiseasesPoaceaeTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26922334

Citation

Kruppa, Klaudia, et al. "McGISH Identification and Phenotypic Description of Leaf Rust and Yellow Rust Resistant Partial Amphiploids Originating From a Wheat × Thinopyrum Synthetic Hybrid Cross." Journal of Applied Genetics, vol. 57, no. 4, 2016, pp. 427-437.

Kruppa K, Türkösi E, Mayer M, et al. McGISH identification and phenotypic description of leaf rust and yellow rust resistant partial amphiploids originating from a wheat × Thinopyrum synthetic hybrid cross. J Appl Genet. 2016;57(4):427-437.

Kruppa, K., Türkösi, E., Mayer, M., Tóth, V., Vida, G., Szakács, É., & Molnár-Láng, M. (2016). McGISH identification and phenotypic description of leaf rust and yellow rust resistant partial amphiploids originating from a wheat × Thinopyrum synthetic hybrid cross. Journal of Applied Genetics, 57(4), 427-437.

Kruppa K, et al. McGISH Identification and Phenotypic Description of Leaf Rust and Yellow Rust Resistant Partial Amphiploids Originating From a Wheat × Thinopyrum Synthetic Hybrid Cross. J Appl Genet. 2016;57(4):427-437. PubMed PMID: 26922334.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - McGISH identification and phenotypic description of leaf rust and yellow rust resistant partial amphiploids originating from a wheat × Thinopyrum synthetic hybrid cross. AU - Kruppa,Klaudia, AU - Türkösi,Edina, AU - Mayer,Marianna, AU - Tóth,Viola, AU - Vida,Gyula, AU - Szakács,Éva, AU - Molnár-Láng,Márta, Y1 - 2016/02/27/ PY - 2016/01/18/received PY - 2016/02/15/accepted PY - 2016/2/29/pubmed PY - 2017/2/7/medline PY - 2016/2/29/entrez KW - FISH KW - Leaf rust resistance KW - Multicolour GISH KW - Partial amphiploid KW - Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid (Agropyron glael) SP - 427 EP - 437 JF - Journal of applied genetics JO - J Appl Genet VL - 57 IS - 4 N2 - A Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid wheatgrass is an excellent source of leaf and stem rust resistance produced by N.V.Tsitsin. Wheat line Mv9kr1 was crossed with this hybrid (Agropyron glael) in Hungary in order to transfer its advantageous agronomic traits into wheat. As the wheat parent was susceptible to leaf rust, the transfer of resistance was easily recognizable in the progenies. Three different partial amphiploid lines with leaf rust resistance were selected from the wheat/Thinopyrum hybrid derivatives by multicolour genomic in situ hybridization. Chromosome counting on the partial amphiploids revealed 58 chromosomes (18 wheatgrass) in line 194, 56 (14 wheatgrass) in line 195 and 54 (12 wheatgrass) in line 196. The wheat chromosomes present in these lines were identified and the wheatgrass chromosomes were characterized by fluorescence in situ hybridization using the repetitive DNA probes Afa-family, pSc119.2 and pTa71. The 3D wheat chromosome was missing from the lines. Molecular marker analysis showed the presence of the Lr24 leaf rust resistance gene in lines 195 and 196. The morphological traits were evaluated in the field during two consecutive seasons in two different locations. SN - 2190-3883 UR - https://www.unboundmedicine.com/medline/citation/26922334/McGISH_identification_and_phenotypic_description_of_leaf_rust_and_yellow_rust_resistant_partial_amphiploids_originating_from_a_wheat_��_Thinopyrum_synthetic_hybrid_cross_ DB - PRIME DP - Unbound Medicine ER -