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Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013.
Virol J 2016; 13:34VJ

Abstract

BACKGROUND

Orf is a contagious disease of sheep, goats and wild ungulates caused by orf virus (ORFV) a member of the genus Parapoxvirus, Poxviridae family. Although orf is endemic in Ethiopia, little attention has been given so far as it is not a notifiable disease by the World Organization for Animal Health. In this work, we have investigated orf outbreaks representing five different geographical locations of Ethiopia, in Amba Giorgis, Gondar zuria, Adet, Debre zeit and Adami Tulu, between 2008 and 2013.

RESULTS

The viral isolation and the sequence analysis of the A32L and the B2L genes of eighteen representative isolates confirmed that sampled animals were infected by ORFVs. The phylogenetic study and the comparative analysis of the deduced amino acid profile suggests that there were two main clusters of ORFV isolates which were responsible for the investigated outbreaks. Additionally the analysis of these two genes showed limited variability to ORFVs encountered elsewhere. This is the first report on the genetic characterization of the ORFV isolates from sheep and goats in Ethiopia.

CONCLUSION

The molecular characterization of Ethiopian ORFV isolates highlighted the circulation of two main clusters causing orf disease in sheep and goats. The use of laboratory based methods and a constant monitoring of Ethiopian ORFV isolates is needed to better understand the dynamic of ORFV circulating in the country and facilitate the implementation of control measures.

Authors+Show Affiliations

Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400, Vienna, Austria. Institute of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Muthgasse 18, A-1190, Vienna, Austria. Research and Diagnostic Laboratories, National Veterinary Institute, P.O. Box 19, Debre Zeit, Ethiopia.Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400, Vienna, Austria.Research and Diagnostic Laboratories, National Veterinary Institute, P.O. Box 19, Debre Zeit, Ethiopia.Research and Diagnostic Laboratories, National Veterinary Institute, P.O. Box 19, Debre Zeit, Ethiopia.Research and Diagnostic Laboratories, National Veterinary Institute, P.O. Box 19, Debre Zeit, Ethiopia.Research and Diagnostic Laboratories, National Veterinary Institute, P.O. Box 19, Debre Zeit, Ethiopia.Institute for Veterinary Disease Control, Austrian Agency for Health and Food Safety, Robert Koch-Gasse 17, A-2340, Mödling, Austria.Institute of Applied Microbiology, University of Natural Resources and Life Sciences, Muthgasse 11, 1190, Vienna, Austria.Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400, Vienna, Austria.Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400, Vienna, Austria. c.lamien@iaea.org.

Pub Type(s)

Historical Article
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26923232

Citation

Gelaye, Esayas, et al. "Molecular Characterization of Orf Virus From Sheep and Goats in Ethiopia, 2008-2013." Virology Journal, vol. 13, 2016, p. 34.
Gelaye E, Achenbach JE, Jenberie S, et al. Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013. Virol J. 2016;13:34.
Gelaye, E., Achenbach, J. E., Jenberie, S., Ayelet, G., Belay, A., Yami, M., ... Lamien, C. E. (2016). Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013. Virology Journal, 13, p. 34. doi:10.1186/s12985-016-0489-3.
Gelaye E, et al. Molecular Characterization of Orf Virus From Sheep and Goats in Ethiopia, 2008-2013. Virol J. 2016 Feb 29;13:34. PubMed PMID: 26923232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013. AU - Gelaye,Esayas, AU - Achenbach,Jenna Elizabeth, AU - Jenberie,Shiferaw, AU - Ayelet,Gelagay, AU - Belay,Alebachew, AU - Yami,Martha, AU - Loitsch,Angelika, AU - Grabherr,Reingard, AU - Diallo,Adama, AU - Lamien,Charles Euloge, Y1 - 2016/02/29/ PY - 2016/01/25/received PY - 2016/02/11/accepted PY - 2016/3/1/entrez PY - 2016/3/1/pubmed PY - 2016/10/14/medline SP - 34 EP - 34 JF - Virology journal JO - Virol. J. VL - 13 N2 - BACKGROUND: Orf is a contagious disease of sheep, goats and wild ungulates caused by orf virus (ORFV) a member of the genus Parapoxvirus, Poxviridae family. Although orf is endemic in Ethiopia, little attention has been given so far as it is not a notifiable disease by the World Organization for Animal Health. In this work, we have investigated orf outbreaks representing five different geographical locations of Ethiopia, in Amba Giorgis, Gondar zuria, Adet, Debre zeit and Adami Tulu, between 2008 and 2013. RESULTS: The viral isolation and the sequence analysis of the A32L and the B2L genes of eighteen representative isolates confirmed that sampled animals were infected by ORFVs. The phylogenetic study and the comparative analysis of the deduced amino acid profile suggests that there were two main clusters of ORFV isolates which were responsible for the investigated outbreaks. Additionally the analysis of these two genes showed limited variability to ORFVs encountered elsewhere. This is the first report on the genetic characterization of the ORFV isolates from sheep and goats in Ethiopia. CONCLUSION: The molecular characterization of Ethiopian ORFV isolates highlighted the circulation of two main clusters causing orf disease in sheep and goats. The use of laboratory based methods and a constant monitoring of Ethiopian ORFV isolates is needed to better understand the dynamic of ORFV circulating in the country and facilitate the implementation of control measures. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/26923232/Molecular_characterization_of_orf_virus_from_sheep_and_goats_in_Ethiopia_2008_2013_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/s12985-016-0489-3 DB - PRIME DP - Unbound Medicine ER -