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Proteomic characterization and comparison of venoms from two elapid snakes (Bungarus multicinctus and Naja atra) from China.
J Proteomics. 2016 Apr 14; 138:83-94.JP

Abstract

Bungarus multicinctus (many-banded krait) and Naja atra (Chinese cobra) are widely distributed and medically important venomous snakes in China; however, their venom proteomic profiles have not been fully compared. Here, we fractionated crude venoms and analyzed them using a combination of proteomic techniques. Three-finger toxins (3-FTx) and phospholipase A2 (PLA2) were most abundant in both species, respectively accounting for 32.6% and 66.4% of total B. multicinctus venom, and 84.3% and 12.2% of total N. atra venom. Venoms from these two species contained one common protein family and six less abundant species-specific protein families. The proteomic profiles of B. multicinctus and N. atra venoms and analysis of toxicological activity in mice suggested that 3-FTx and PLA2 are the major contributors to clinical symptoms caused by envenomation. The venoms differed in enzymatic activity, likely the result of inter-specific variation in the amount of related venom components. Antivenomics assessment revealed that a small number of venom components (3-FTxs and PLA2s in B. multicinctus, and 3-FTxs in N. atra) could not be immunocaptured completely, suggesting that we should pay attention to enhancing the immune response of these components in designing commercial antivenoms for B. multicinctus and N. atra.

BIOLOGICAL SIGNIFICANCE

The proteomic profiles of venoms from two medically important snake species - B. multicinctus and N. atra - have been explored. Quantitative and qualitative differences are evident in both venoms when proteomic profiles and transcriptomic results are compared; this is a reminder that combined approaches are needed to explore the precise composition of snake venom. Two protein families (3-FTx and PLA2) of high abundance in these snake venoms are major players in the biochemical and pharmacological effects of envenomation. Elucidation of the proteomic profiles of these snake venoms is helpful in understanding composition-function relationships and will facilitate the clinical application of antivenoms.

Authors+Show Affiliations

Hangzhou Key Laboratory for Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China.Hangzhou Key Laboratory for Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China. Electronic address: gaojf308@163.com.Hangzhou Key Laboratory for Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China.Hangzhou Key Laboratory for Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China.Hangzhou Key Laboratory for Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China.Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, Jiangsu, China.Hangzhou Key Laboratory for Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China.Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, Jiangsu, China. Electronic address: xji@mail.hz.zj.cn.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26924299

Citation

Shan, Lin-Lin, et al. "Proteomic Characterization and Comparison of Venoms From Two Elapid Snakes (Bungarus Multicinctus and Naja Atra) From China." Journal of Proteomics, vol. 138, 2016, pp. 83-94.
Shan LL, Gao JF, Zhang YX, et al. Proteomic characterization and comparison of venoms from two elapid snakes (Bungarus multicinctus and Naja atra) from China. J Proteomics. 2016;138:83-94.
Shan, L. L., Gao, J. F., Zhang, Y. X., Shen, S. S., He, Y., Wang, J., Ma, X. M., & Ji, X. (2016). Proteomic characterization and comparison of venoms from two elapid snakes (Bungarus multicinctus and Naja atra) from China. Journal of Proteomics, 138, 83-94. https://doi.org/10.1016/j.jprot.2016.02.028
Shan LL, et al. Proteomic Characterization and Comparison of Venoms From Two Elapid Snakes (Bungarus Multicinctus and Naja Atra) From China. J Proteomics. 2016 Apr 14;138:83-94. PubMed PMID: 26924299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic characterization and comparison of venoms from two elapid snakes (Bungarus multicinctus and Naja atra) from China. AU - Shan,Lin-Lin, AU - Gao,Jian-Fang, AU - Zhang,Yan-Xia, AU - Shen,Shan-Shan, AU - He,Ying, AU - Wang,Jin, AU - Ma,Xiao-Mei, AU - Ji,Xiang, Y1 - 2016/02/26/ PY - 2015/09/22/received PY - 2016/02/21/revised PY - 2016/02/24/accepted PY - 2016/3/1/entrez PY - 2016/3/1/pubmed PY - 2016/12/20/medline KW - Antivenomics KW - Bungarus multicinctus KW - Functional role KW - Naja atra KW - Proteomic profile KW - Snake venom SP - 83 EP - 94 JF - Journal of proteomics JO - J Proteomics VL - 138 N2 - UNLABELLED: Bungarus multicinctus (many-banded krait) and Naja atra (Chinese cobra) are widely distributed and medically important venomous snakes in China; however, their venom proteomic profiles have not been fully compared. Here, we fractionated crude venoms and analyzed them using a combination of proteomic techniques. Three-finger toxins (3-FTx) and phospholipase A2 (PLA2) were most abundant in both species, respectively accounting for 32.6% and 66.4% of total B. multicinctus venom, and 84.3% and 12.2% of total N. atra venom. Venoms from these two species contained one common protein family and six less abundant species-specific protein families. The proteomic profiles of B. multicinctus and N. atra venoms and analysis of toxicological activity in mice suggested that 3-FTx and PLA2 are the major contributors to clinical symptoms caused by envenomation. The venoms differed in enzymatic activity, likely the result of inter-specific variation in the amount of related venom components. Antivenomics assessment revealed that a small number of venom components (3-FTxs and PLA2s in B. multicinctus, and 3-FTxs in N. atra) could not be immunocaptured completely, suggesting that we should pay attention to enhancing the immune response of these components in designing commercial antivenoms for B. multicinctus and N. atra. BIOLOGICAL SIGNIFICANCE: The proteomic profiles of venoms from two medically important snake species - B. multicinctus and N. atra - have been explored. Quantitative and qualitative differences are evident in both venoms when proteomic profiles and transcriptomic results are compared; this is a reminder that combined approaches are needed to explore the precise composition of snake venom. Two protein families (3-FTx and PLA2) of high abundance in these snake venoms are major players in the biochemical and pharmacological effects of envenomation. Elucidation of the proteomic profiles of these snake venoms is helpful in understanding composition-function relationships and will facilitate the clinical application of antivenoms. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/26924299/Proteomic_characterization_and_comparison_of_venoms_from_two_elapid_snakes__Bungarus_multicinctus_and_Naja_atra__from_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(16)30050-1 DB - PRIME DP - Unbound Medicine ER -