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Methodological approach and diagnostic usefulness of a new assay for anti-thyroid peroxidase autoantibodies.
Ann Biol Clin (Paris). 1989; 47(9):541-5.AB

Abstract

Although thyroid microsomal antibodies (anti-MAb) have been recently proven to be directly to thyroid peroxidase (TPO), current methods for MAb detection still employ unpurified microsomal fractions. The authors have therefore developed and evaluated a specific radioimmunoassay (RIA) for autoantibodies to TPO (anti-TPO Ab) based on competitive inhibition of 125I-TPO to an anti-TPO monoclonal antibody coated on plastic microtiter wells (RIA-1) or tubes (RIA-2). Preliminary experiments showed that both assays were able to specifically detect anti-TPO Ab, while negative results were obtained with normal sera and with sera containing other organ- and non-organ-specific autoantibodies including anti-thyroglobulin antibodies (anti-TgAb). No significant difference in sensitivity, specificity and reproducibility was found between RIA-1 and RIA-2, and the results obtained with the two techniques were therefore pooled together. Anti-TPO Ab were then assayed in 110 normal controls and in 441 patients with different autoimmune (AITD) or non-autoimmune (N-AITD) thyroid diseases and compared to anti-M Ab as assessed by passive hemagglutination (PH). Positive anti-TPO Ab were observed in 4/110 (3.6 p. cent) normal controls, 88/117 (80 p. cent) patients with Graves' disease, 122/123 with Hashimoto's thyroiditis or idiopathic myxedema and 21/201 (10.4 p. cent) with miscellaneous N-AITD. A highly significant positive correlation (r = 0.91, p less than 0.0001) was found between anti-MAb by PH and anti-TPO Ab by RIA ;discrepant results were limited to sera with negative or low (1/100-1/400) anti-M Ab titers.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Cattedra di Endocrinologia, Università di Pisa, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2692481

Citation

Mariotti, S, et al. "Methodological Approach and Diagnostic Usefulness of a New Assay for Anti-thyroid Peroxidase Autoantibodies." Annales De Biologie Clinique, vol. 47, no. 9, 1989, pp. 541-5.
Mariotti S, Ruf J, Caturegli P, et al. Methodological approach and diagnostic usefulness of a new assay for anti-thyroid peroxidase autoantibodies. Ann Biol Clin (Paris). 1989;47(9):541-5.
Mariotti, S., Ruf, J., Caturegli, P., Rossi, V., Boniolo, A., Piccolo, P., Carayon, P., & Pinchera, A. (1989). Methodological approach and diagnostic usefulness of a new assay for anti-thyroid peroxidase autoantibodies. Annales De Biologie Clinique, 47(9), 541-5.
Mariotti S, et al. Methodological Approach and Diagnostic Usefulness of a New Assay for Anti-thyroid Peroxidase Autoantibodies. Ann Biol Clin (Paris). 1989;47(9):541-5. PubMed PMID: 2692481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methodological approach and diagnostic usefulness of a new assay for anti-thyroid peroxidase autoantibodies. AU - Mariotti,S, AU - Ruf,J, AU - Caturegli,P, AU - Rossi,V, AU - Boniolo,A, AU - Piccolo,P, AU - Carayon,P, AU - Pinchera,A, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 541 EP - 5 JF - Annales de biologie clinique JO - Ann. Biol. Clin. (Paris) VL - 47 IS - 9 N2 - Although thyroid microsomal antibodies (anti-MAb) have been recently proven to be directly to thyroid peroxidase (TPO), current methods for MAb detection still employ unpurified microsomal fractions. The authors have therefore developed and evaluated a specific radioimmunoassay (RIA) for autoantibodies to TPO (anti-TPO Ab) based on competitive inhibition of 125I-TPO to an anti-TPO monoclonal antibody coated on plastic microtiter wells (RIA-1) or tubes (RIA-2). Preliminary experiments showed that both assays were able to specifically detect anti-TPO Ab, while negative results were obtained with normal sera and with sera containing other organ- and non-organ-specific autoantibodies including anti-thyroglobulin antibodies (anti-TgAb). No significant difference in sensitivity, specificity and reproducibility was found between RIA-1 and RIA-2, and the results obtained with the two techniques were therefore pooled together. Anti-TPO Ab were then assayed in 110 normal controls and in 441 patients with different autoimmune (AITD) or non-autoimmune (N-AITD) thyroid diseases and compared to anti-M Ab as assessed by passive hemagglutination (PH). Positive anti-TPO Ab were observed in 4/110 (3.6 p. cent) normal controls, 88/117 (80 p. cent) patients with Graves' disease, 122/123 with Hashimoto's thyroiditis or idiopathic myxedema and 21/201 (10.4 p. cent) with miscellaneous N-AITD. A highly significant positive correlation (r = 0.91, p less than 0.0001) was found between anti-MAb by PH and anti-TPO Ab by RIA ;discrepant results were limited to sera with negative or low (1/100-1/400) anti-M Ab titers.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0003-3898 UR - https://www.unboundmedicine.com/medline/citation/2692481/Methodological_approach_and_diagnostic_usefulness_of_a_new_assay_for_anti_thyroid_peroxidase_autoantibodies_ L2 - https://medlineplus.gov/thyroiddiseases.html DB - PRIME DP - Unbound Medicine ER -