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Anti-obesity effect of milk fermented by Lactobacillus plantarum NCDC 625 alone and in combination with herbs on high fat diet fed C57BL/6J mice.
Benef Microbes 2016; 7(3):375-85BM

Abstract

The effect of dietary supplementation of milk fermented with indigenous probiotic Lactobacillus plantarum (LP625) alone and in combination with herbs (Aloe vera and Gymnema sylvestre) was investigated on high fat diet (HFD, 60 kcal% fat) fed mice for 12 weeks. Administration of LP625 alone or in combination with both herbs lowered the final body weight, however, a significant difference was observed with LP625 supplemented Gymnema sylvestre only as compared to the HFD fed group (25.06±0.18 vs 27.29±0.72 g, P<0.05). Similarly, the epididymal fat mass, fasting blood glucose and serum insulin levels were significantly (P<0.05) decreased by all treatment groups. In addition, a protective effect against the rise in serum and liver triglycerides, and in liver total cholesterol levels was found with the consumption of LP625 alone or in combination with herbs. Furthermore, the HFD fed mice showed a remarkable increase in the epididymal fat cell size, whereas administration of LP625 alone or in combination with herbs exhibited a significant decrease in the size. Finally, a significant increase in the relative mRNA expression of thermogenic proteins, i.e. uncoupling protein-2 (UCP-2, 1.16±0.25 fold change, P<0.05) and a decrease in pro-inflammatory markers, such as tumour necrosis factor-α and interleukin-6 (1.55±0.18 and 3.10±0.58 fold change, respectively, P<0.05) were due to LP625 supplementation in the HFD fed group. This shows that LP625 alone or supplemented with herbs seems to protect against diet induced obesity by decreasing the body and epididymal fat weight through upregulation of UCP-2 expression and reduced expression of pro-inflammatory cytokines.

Authors+Show Affiliations

1 Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, India.1 Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, India.1 Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, India.1 Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, India.1 Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, India.1 Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, India.2 Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26925603

Citation

Pothuraju, R, et al. "Anti-obesity Effect of Milk Fermented By Lactobacillus Plantarum NCDC 625 Alone and in Combination With Herbs On High Fat Diet Fed C57BL/6J Mice." Beneficial Microbes, vol. 7, no. 3, 2016, pp. 375-85.
Pothuraju R, Sharma RK, Kavadi PK, et al. Anti-obesity effect of milk fermented by Lactobacillus plantarum NCDC 625 alone and in combination with herbs on high fat diet fed C57BL/6J mice. Benef Microbes. 2016;7(3):375-85.
Pothuraju, R., Sharma, R. K., Kavadi, P. K., Chagalamarri, J., Jangra, S., Bhakri, G., & De, S. (2016). Anti-obesity effect of milk fermented by Lactobacillus plantarum NCDC 625 alone and in combination with herbs on high fat diet fed C57BL/6J mice. Beneficial Microbes, 7(3), pp. 375-85. doi:10.3920/BM2015.0083.
Pothuraju R, et al. Anti-obesity Effect of Milk Fermented By Lactobacillus Plantarum NCDC 625 Alone and in Combination With Herbs On High Fat Diet Fed C57BL/6J Mice. Benef Microbes. 2016;7(3):375-85. PubMed PMID: 26925603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-obesity effect of milk fermented by Lactobacillus plantarum NCDC 625 alone and in combination with herbs on high fat diet fed C57BL/6J mice. AU - Pothuraju,R, AU - Sharma,R K, AU - Kavadi,P K, AU - Chagalamarri,J, AU - Jangra,S, AU - Bhakri,G, AU - De,S, Y1 - 2016/03/01/ PY - 2016/3/2/entrez PY - 2016/3/2/pubmed PY - 2017/1/18/medline KW - Aloe vera KW - Gymnema sylvestre KW - Lactobacillus plantarum KW - high fat diet KW - obesity SP - 375 EP - 85 JF - Beneficial microbes JO - Benef Microbes VL - 7 IS - 3 N2 - The effect of dietary supplementation of milk fermented with indigenous probiotic Lactobacillus plantarum (LP625) alone and in combination with herbs (Aloe vera and Gymnema sylvestre) was investigated on high fat diet (HFD, 60 kcal% fat) fed mice for 12 weeks. Administration of LP625 alone or in combination with both herbs lowered the final body weight, however, a significant difference was observed with LP625 supplemented Gymnema sylvestre only as compared to the HFD fed group (25.06±0.18 vs 27.29±0.72 g, P<0.05). Similarly, the epididymal fat mass, fasting blood glucose and serum insulin levels were significantly (P<0.05) decreased by all treatment groups. In addition, a protective effect against the rise in serum and liver triglycerides, and in liver total cholesterol levels was found with the consumption of LP625 alone or in combination with herbs. Furthermore, the HFD fed mice showed a remarkable increase in the epididymal fat cell size, whereas administration of LP625 alone or in combination with herbs exhibited a significant decrease in the size. Finally, a significant increase in the relative mRNA expression of thermogenic proteins, i.e. uncoupling protein-2 (UCP-2, 1.16±0.25 fold change, P<0.05) and a decrease in pro-inflammatory markers, such as tumour necrosis factor-α and interleukin-6 (1.55±0.18 and 3.10±0.58 fold change, respectively, P<0.05) were due to LP625 supplementation in the HFD fed group. This shows that LP625 alone or supplemented with herbs seems to protect against diet induced obesity by decreasing the body and epididymal fat weight through upregulation of UCP-2 expression and reduced expression of pro-inflammatory cytokines. SN - 1876-2891 UR - https://www.unboundmedicine.com/medline/citation/26925603/Anti_obesity_effect_of_milk_fermented_by_Lactobacillus_plantarum_NCDC_625_alone_and_in_combination_with_herbs_on_high_fat_diet_fed_C57BL/6J_mice_ L2 - http://www.wageningenacademic.com/doi/full/10.3920/BM2015.0083?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -