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Distinct transcriptomic responses of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles.
Environ Pollut. 2016 Jun; 213:314-321.EP

Abstract

Manufactured nanoparticles (MNP) rapidly undergo aging processes once released from products. Silver sulfide (Ag2S) is the major transformation product formed during the wastewater treatment process for Ag-MNP. We examined toxicogenomic responses of pristine Ag-MNP, sulfidized Ag-MNP (sAg-MNP), and AgNO3 to a model soil organism, Caenorhabditis elegans. Transcriptomic profiling of nematodes which were exposed at the EC30 for reproduction for AgNO3, Ag-MNP, and sAg-MNP resulted in 571 differentially expressed genes. We independently verified expression of 4 genes (numr-1, rol-8, col-158, and grl-20) using qRT-PCR. Only 11% of differentially expressed genes were common among the three treatments. Gene ontology enrichment analysis also revealed that Ag-MNP and sAg-MNP had distinct toxicity mechanisms and did not share any of the biological processes. The processes most affected by Ag-MNP relate to metabolism, while those processes most affected by sAg-MNP relate to molting and the cuticle, and the most impacted processes for AgNO3 exposed nematodes was stress related. Additionally, as observed from qRT-PCR and mutant experiments, the responses to sAg-MNP were distinct from AgNO3 while some of the effects of pristine MNP were similar to AgNO3, suggesting that effects from Ag-MNP is partially due to dissolved silver ions.

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Authors+Show Affiliations

Starnes DL
Department of Plant and Soil Sciences, University of Kentucky, 1100 South Limestone Street, Lexington, KY 40546, United States.
Lichtenberg SS
Department of Plant and Soil Sciences, University of Kentucky, 1100 South Limestone Street, Lexington, KY 40546, United States; Center for Environmental Implications of NanoTechnology (CEINT), P.O. Box 90287, Duke University, Durham, NC 27708-0287, United States.
Unrine JM
Department of Plant and Soil Sciences, University of Kentucky, 1100 South Limestone Street, Lexington, KY 40546, United States; Center for Environmental Implications of NanoTechnology (CEINT), P.O. Box 90287, Duke University, Durham, NC 27708-0287, United States.
Starnes CP
Department of Statistics, University of Kentucky, 725 Rose Street, MDS Building 305, Lexington, KY 40536, United States.
Oostveen EK
Department of Plant and Soil Sciences, University of Kentucky, 1100 South Limestone Street, Lexington, KY 40546, United States.
Lowry GV
Center for Environmental Implications of NanoTechnology (CEINT), P.O. Box 90287, Duke University, Durham, NC 27708-0287, United States; Department of Civil & Environmental Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, United States.
Bertsch PM
Department of Plant and Soil Sciences, University of Kentucky, 1100 South Limestone Street, Lexington, KY 40546, United States; Center for Environmental Implications of NanoTechnology (CEINT), P.O. Box 90287, Duke University, Durham, NC 27708-0287, United States; Division of Land and Water, CSIRO, Ecosciences Precinct, Brisbane, QLD, Australia.
Tsyusko OV
Department of Plant and Soil Sciences, University of Kentucky, 1100 South Limestone Street, Lexington, KY 40546, United States; Center for Environmental Implications of NanoTechnology (CEINT), P.O. Box 90287, Duke University, Durham, NC 27708-0287, United States. Electronic address: olga.tsyusko@uky.edu.

MeSH

AnimalsCaenorhabditis elegansMetal NanoparticlesSilverSilver CompoundsSilver NitrateTranscriptomeWastewater

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26925754

Citation

Starnes, Daniel L., et al. "Distinct Transcriptomic Responses of Caenorhabditis Elegans to Pristine and Sulfidized Silver Nanoparticles." Environmental Pollution (Barking, Essex : 1987), vol. 213, 2016, pp. 314-321.
Starnes DL, Lichtenberg SS, Unrine JM, et al. Distinct transcriptomic responses of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles. Environ Pollut. 2016;213:314-321.
Starnes, D. L., Lichtenberg, S. S., Unrine, J. M., Starnes, C. P., Oostveen, E. K., Lowry, G. V., Bertsch, P. M., & Tsyusko, O. V. (2016). Distinct transcriptomic responses of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles. Environmental Pollution (Barking, Essex : 1987), 213, 314-321. https://doi.org/10.1016/j.envpol.2016.01.020
Starnes DL, et al. Distinct Transcriptomic Responses of Caenorhabditis Elegans to Pristine and Sulfidized Silver Nanoparticles. Environ Pollut. 2016;213:314-321. PubMed PMID: 26925754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct transcriptomic responses of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles. AU - Starnes,Daniel L, AU - Lichtenberg,Stuart S, AU - Unrine,Jason M, AU - Starnes,Catherine P, AU - Oostveen,Emily K, AU - Lowry,Gregory V, AU - Bertsch,Paul M, AU - Tsyusko,Olga V, Y1 - 2016/02/27/ PY - 2015/09/16/received PY - 2015/12/23/revised PY - 2016/01/07/accepted PY - 2016/3/2/entrez PY - 2016/3/2/pubmed PY - 2017/1/18/medline KW - Antimicrobial KW - Nanomaterial KW - Soil KW - Toxicogenomics KW - Wastewater SP - 314 EP - 321 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 213 N2 - Manufactured nanoparticles (MNP) rapidly undergo aging processes once released from products. Silver sulfide (Ag2S) is the major transformation product formed during the wastewater treatment process for Ag-MNP. We examined toxicogenomic responses of pristine Ag-MNP, sulfidized Ag-MNP (sAg-MNP), and AgNO3 to a model soil organism, Caenorhabditis elegans. Transcriptomic profiling of nematodes which were exposed at the EC30 for reproduction for AgNO3, Ag-MNP, and sAg-MNP resulted in 571 differentially expressed genes. We independently verified expression of 4 genes (numr-1, rol-8, col-158, and grl-20) using qRT-PCR. Only 11% of differentially expressed genes were common among the three treatments. Gene ontology enrichment analysis also revealed that Ag-MNP and sAg-MNP had distinct toxicity mechanisms and did not share any of the biological processes. The processes most affected by Ag-MNP relate to metabolism, while those processes most affected by sAg-MNP relate to molting and the cuticle, and the most impacted processes for AgNO3 exposed nematodes was stress related. Additionally, as observed from qRT-PCR and mutant experiments, the responses to sAg-MNP were distinct from AgNO3 while some of the effects of pristine MNP were similar to AgNO3, suggesting that effects from Ag-MNP is partially due to dissolved silver ions. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/26925754/Distinct_transcriptomic_responses_of_Caenorhabditis_elegans_to_pristine_and_sulfidized_silver_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -