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[Pulsatilla decoction inhibits vulvovaginal Candida albicans proliferation and reduces inflammatory cytokine levels in vulvovaginal candidiasis mice].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Feb; 32(2):153-7.XB

Abstract

OBJECTIVE

To explore the possible regulatory effect of Pulsatilla decoction on Th17 cells and inflammatory cytokines of vulvovaginal candidiasis (VVC) mice.

METHODS

Seventy-two female Kunming mice were randomly assigned into six groups: a blank control group, a VVC model group, a fluconazole group and three Pulsatilla decoction groups (dose levels: 22.5, 15.0 and 7.5 g/kg, respectively). The VVC mouse models were established by vaginal inoculation with Candida albicans (C. albicans) in female mice in pseudoestrus state caused by estradiol injection. After 7-day treatment on VVC mice, the vaginal C. albicans burden was assessed using dilution spread plate method; the vaginal C. albicans morphology was observed by Gram staining method; the levels of interleukin 6 (IL-6), IL-17, IL-21 and tumor necrosis factor α (TNF-α) in sera were detected by ELISA. The content of the transcription factor retinoid related orphan receptor gamma t (RORγt) in vaginal tissues was detected by immunohistochemistry.

RESULTS

The VVC mouse models were successfully developed. After treatment, the vaginal C. albicans burden of the fluconazole group and 22.5 g/kg Pulsatilla decoction group dropped significantly compared with that of the VVC model group. Gram staining showed that the VVC mice had lots of C. albicans hyphae in vaginal discharge, that 7.5 g/kg Pulsatilla decoction group remained the mycelia-phase C. albicans, and that 15.0 g/kg Pulsatilla decoction group had the majority of yeast-phase C. albicans and a few of mycelia-phase, while no hyphae and only very few of yeast-phase C. albicans were observed in 22.5 g/kg Pulsatilla decoction group and fluconazole group. After 7-day treatment, compared with the model group, the levels of IL-6, IL- 17, IL-21 and TNF-α in the sera of the fluconazole group, 15.0 and 22.5 g/kg Pulsatilla decoction groups were reduced significantly and the levels of RORγt in the vaginal tissues of the fluconazole group, 15.0 and 22.5 g/kg Pulsatilla decoction groups also decreased significantly.

CONCLUSION

Pulsatilla decoction could inhibit the proliferation of vulvovaginal C. albicans and reduces the levels of inflammatory cytokines in VVC mice.

Authors+Show Affiliations

School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine; Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China.School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine; Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China.School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine; Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China.School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine; Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China.School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine; Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China.School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine; Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China.School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine; Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China.School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine; Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China. *Corresponding author, E-mail: ahwcz63@sina.com.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

26927371

Citation

Xia, Dan, et al. "[Pulsatilla Decoction Inhibits Vulvovaginal Candida Albicans Proliferation and Reduces Inflammatory Cytokine Levels in Vulvovaginal Candidiasis Mice]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 32, no. 2, 2016, pp. 153-7.
Xia D, Zhang M, Shi G, et al. [Pulsatilla decoction inhibits vulvovaginal Candida albicans proliferation and reduces inflammatory cytokine levels in vulvovaginal candidiasis mice]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016;32(2):153-7.
Xia, D., Zhang, M., Shi, G., Xu, Z., Wu, D., Shao, J., Wang, T., & Wang, C. (2016). [Pulsatilla decoction inhibits vulvovaginal Candida albicans proliferation and reduces inflammatory cytokine levels in vulvovaginal candidiasis mice]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 32(2), 153-7.
Xia D, et al. [Pulsatilla Decoction Inhibits Vulvovaginal Candida Albicans Proliferation and Reduces Inflammatory Cytokine Levels in Vulvovaginal Candidiasis Mice]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016;32(2):153-7. PubMed PMID: 26927371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Pulsatilla decoction inhibits vulvovaginal Candida albicans proliferation and reduces inflammatory cytokine levels in vulvovaginal candidiasis mice]. AU - Xia,Dan, AU - Zhang,Mengxiang, AU - Shi,Gaoxiang, AU - Xu,Zhiqing, AU - Wu,Daqiang, AU - Shao,Jing, AU - Wang,Tianming, AU - Wang,Changzhong, PY - 2016/3/2/entrez PY - 2016/3/2/pubmed PY - 2016/7/20/medline SP - 153 EP - 7 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 32 IS - 2 N2 - OBJECTIVE: To explore the possible regulatory effect of Pulsatilla decoction on Th17 cells and inflammatory cytokines of vulvovaginal candidiasis (VVC) mice. METHODS: Seventy-two female Kunming mice were randomly assigned into six groups: a blank control group, a VVC model group, a fluconazole group and three Pulsatilla decoction groups (dose levels: 22.5, 15.0 and 7.5 g/kg, respectively). The VVC mouse models were established by vaginal inoculation with Candida albicans (C. albicans) in female mice in pseudoestrus state caused by estradiol injection. After 7-day treatment on VVC mice, the vaginal C. albicans burden was assessed using dilution spread plate method; the vaginal C. albicans morphology was observed by Gram staining method; the levels of interleukin 6 (IL-6), IL-17, IL-21 and tumor necrosis factor α (TNF-α) in sera were detected by ELISA. The content of the transcription factor retinoid related orphan receptor gamma t (RORγt) in vaginal tissues was detected by immunohistochemistry. RESULTS: The VVC mouse models were successfully developed. After treatment, the vaginal C. albicans burden of the fluconazole group and 22.5 g/kg Pulsatilla decoction group dropped significantly compared with that of the VVC model group. Gram staining showed that the VVC mice had lots of C. albicans hyphae in vaginal discharge, that 7.5 g/kg Pulsatilla decoction group remained the mycelia-phase C. albicans, and that 15.0 g/kg Pulsatilla decoction group had the majority of yeast-phase C. albicans and a few of mycelia-phase, while no hyphae and only very few of yeast-phase C. albicans were observed in 22.5 g/kg Pulsatilla decoction group and fluconazole group. After 7-day treatment, compared with the model group, the levels of IL-6, IL- 17, IL-21 and TNF-α in the sera of the fluconazole group, 15.0 and 22.5 g/kg Pulsatilla decoction groups were reduced significantly and the levels of RORγt in the vaginal tissues of the fluconazole group, 15.0 and 22.5 g/kg Pulsatilla decoction groups also decreased significantly. CONCLUSION: Pulsatilla decoction could inhibit the proliferation of vulvovaginal C. albicans and reduces the levels of inflammatory cytokines in VVC mice. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/26927371/[Pulsatilla_decoction_inhibits_vulvovaginal_Candida_albicans_proliferation_and_reduces_inflammatory_cytokine_levels_in_vulvovaginal_candidiasis_mice]_ L2 - https://antibodies.cancer.gov/detail/CPTC-IL6-1 DB - PRIME DP - Unbound Medicine ER -